Comparing Reagents Used on Applied Biosystem’s 3730xl
Reagents are an integral part of sequencing on Applied Biosystems (ABI) 3730xl. They allow the samples to move through the machine with ease, providing clarity and quality results. But are all reagents created equal? We compared 5x Sequencing Buffer, Dye Terminator cycle sequencing reagent, 10x Running Buffer, and Sequencing Polymer from Edge Biosystems (Edge), ABI, and McLab in order to determine how well each of them performed by evaluating array images, raw fluorescence signal, read clarity, peak uniformity, and sequencing start point.
Twelve PCR products and four plasmid samples underwent cycle sequencing using Dye Terminator cycle sequencing reagent and Sequencing Buffer from each of the three companies: Edge, ABI, and McLab. Each of the three groups of 16 samples were sequenced using Edge Running Buffer and Polymer, ABI Running Buffer and Polymer, and McLab Running Buffer and Polymer. Additionally, each group of 16 samples were also sequenced using each combination of Edge, ABI, and McLab Running Buffer with Edge, ABI, and McLab Polymer. For example, 16 samples prepared with Edge Dye Terminator and Sequencing Buffer were sequenced using: Edge Running Buffer/ABI Polymer, Edge Running Buffer/McLab Polymer, ABI Running Buffer/Edge Polymer, ABI Running Buffer/McLab Polymer, McLab Running Buffer/ABI Polymer, and McLab Running Buffer/Edge Polymer, and so on. After testing, we looked at array images for background clarity.
We compared traces for signal strength and read consistency, and we viewed the sample start point to see when each sequence began. Overall, we determined that all of the reagents were comparable, with Edge and ABI performing slightly better in signal strength, read clarity, and earlier start points.
kolgene
2X PCR Taq MasterMix |
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| G013 | ABM | 5.0 ml (200 Rxns) | EUR 104.4 |
Kodaq 2X PCR MasterMix |
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| G497 | ABM | 5.0 ml (200 Rxns) | EUR 189.6 |
2X PCR TaqFast MasterMix |
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| G280 | ABM | 5.0 ml (200 Rxns) | EUR 160.8 |
BlasTaq 2X PCR MasterMix |
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| G895 | ABM | 800 rxn (10.0 ml) | EUR 275 |
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Description: BlasTaq™ 2X PCR MasterMix is a ready-to-use MasterMix containing abm’s BlasTaq™ DNA Polymerase in a uniquely-formulated buffer with gel loading dye. This strategicallyengineered, next generation Taq Polymerase provides rapid extension rates and robust performance. With specialized reaction conditions, this polymerase provides increased processivity, yields, and sensitivity, while shortening reaction times by up to 70%, compared to wild-type Taq DNA polymerase. BlasTaq™ has 5’-3’ polymerase and 5’-3’ exonuclease activities, lacks 3’-5’ exonuclease activity, and produces 3’-dA-tailed amplicons. PCR products made with BlasTaq™ can be used with TA cloning vectors. |
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BlasTaq 2X PCR MasterMix |
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| E36G895 | EnoGene | 800 rxn (10.0 ml) | EUR 342.86 |
BlasTaq 2X PCR MasterMix |
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| MBS8580078-5x800Reactions | MyBiosource | 5x800Reactions | EUR 2015 |
BlasTaq 2X PCR MasterMix |
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| MBS8580078-800Reactions | MyBiosource | 800Reactions | EUR 440 |
BlasTaq 2X PCR MasterMix |
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| P895-1 | ABM | 100 rxn (1.25 ml) | EUR 60 |
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Description: BlasTaq™ 2X PCR MasterMix is a ready-to-use MasterMix containing abm’s BlasTaq™ DNA Polymerase in a uniquely-formulated buffer with gel loading dye. This strategicallyengineered, next generation Taq Polymerase provides rapid extension rates and robust performance. With specialized reaction conditions, this polymerase provides increased processivity, yields, and sensitivity, while shortening reaction times by up to 70%, compared to wild-type Taq DNA polymerase. BlasTaq™ has 5’-3’ polymerase and 5’-3’ exonuclease activities, lacks 3’-5’ exonuclease activity, and produces 3’-dA-tailed amplicons. PCR products made with BlasTaq™ can be used with TA cloning vectors. |
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2X PCR HotStart MasterMix |
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| G906 | ABM | 5.0 ml (200 Rxns) | EUR 189.6 |
Bloodirect 2X PCR MasterMix |
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| G465 | ABM | 5.0 ml (200 Rxns) | EUR 166.8 |
2X PCR Taq Plus MasterMix |
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| G901 | ABM | 25.0 ml (1000 Rxns) | EUR 358.8 |
2X PCR Taq Plus MasterMix |
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| G014 | ABM | 5.0 ml (200 Rxns) | EUR 127.2 |
2X PCR Bestaq™ MasterMix |
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| G464 | ABM | 5.0 ml (200 Rxns) | EUR 189.6 |
MegaFi Fidelity 2X PCR MasterMix |
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| E36G897 | EnoGene | 800 rxn (10.0 ml) | EUR 414.29 |
EzWay Multiplex PCR MasterMix (2x) |
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| K0567810 | KOMA BIOTECH | 1ml | EUR 94 |
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Description: https://komabiotech.co.kr/labiskoma/datasheet/K0567810,K0567815.pdf |
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EzWay Multiplex PCR MasterMix (2x) |
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| K0567815 | KOMA BIOTECH | 5ml | EUR 322 |
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Description: https://komabiotech.co.kr/labiskoma/datasheet/K0567810,K0567815.pdf |
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MegaFi Fidelity 2X PCR MasterMix |
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| MBS8580080-5x800Reactions | MyBiosource | 5x800Reactions | EUR 2365 |
MegaFi Fidelity 2X PCR MasterMix |
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| MBS8580080-800Reactions | MyBiosource | 800Reactions | EUR 515 |
2X PCR SensTaq HotStart MasterMix |
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| G939 | ABM | 5.0 ml (200 Rxns) | EUR 189.6 |
1ml Precision FAST qPCR Master Mix |
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| Z-PFAST-1ML | Novacyt Group | 1ml | EUR 90 |
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Description: Precision®FAST |
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2ml Precision FAST qPCR Master Mix |
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| Z-PFAST-2ML | Novacyt Group | 2ml | EUR 161 |
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Description: Precision®FAST |
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2X PCR Taq MasterMix with dye |
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| G900-dye | ABM | 25.0 ml (1000 Rxns) | EUR 246 |
2X PCR Taq MasterMix with dye |
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| G013-dye | ABM | 5.0 ml (200 Rxns) | EUR 104.4 |
EzWay Hot Taq PCR MasterMix (2x) |
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| K0567510 | KOMA BIOTECH | 1ml | EUR 68 |
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Description: https://komabiotech.co.kr/labiskoma/datasheet/K0567510,K0567520.pdf |
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Kodaq 2X PCR MasterMix with dye |
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| G497-dye | ABM | 5.0 ml (200 Rxns) | EUR 189.6 |
2x HiFi Hot Start PCR MasterMix |
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| MBS278020-1mL | MyBiosource | 1mL | EUR 160 |
2x HiFi Hot Start PCR MasterMix |
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| MBS278020-5mL | MyBiosource | 5mL | EUR 270 |
1ml Precision FAST CL qPCR Master Mix |
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| Z-PFAST-CL-1ML | Novacyt Group | 1ml | EUR 90 |
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Description: Precision®FAST |
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2ml Precision FAST CL qPCR Master Mix |
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| Z-PFAST-CL-2ML | Novacyt Group | 2ml | EUR 161 |
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Description: Precision®FAST |
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1ml Precision FAST LR qPCR Master Mix |
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| Z-PFAST-LR-1ML | Novacyt Group | 1ml | EUR 90 |
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Description: Precision®FAST |
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2X PCR TaqFast MasterMix with dye |
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| G280-dye | ABM | 5.0 ml (200 Rxns) | EUR 160.8 |
EzWay Direct Taq PCR MasterMix (2x) |
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| K0568010 | KOMA BIOTECH | 1ml | EUR 108 |
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Description: https://komabiotech.co.kr/labiskoma/datasheet/K0568010,K0568020,K0568210,K0569020,K0567840.pdf |
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Blue dye for adding to 1ml Precision Master Mix |
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| Z-BLUEDYE-1ML | Novacyt Group | 8µl | EUR 33 |
Blue dye for adding to 2ml Precision Master Mix |
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| Z-BLUEDYE-2ML | Novacyt Group | 12µl | EUR 38 |
Blue dye for adding to 5ml Precision Master Mix |
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| Z-BLUEDYE-5ML | Novacyt Group | 30µl | EUR 46 |
2X PCR HotStart MasterMix with dye |
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| G906-dye | ABM | 5.0 ml (200 Rxns) | EUR 189.6 |
2 ml Precision FAST LR qPCR Master Mix |
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| Z-PFAST-LR-2ML | Novacyt Group | 2ml | EUR 161 |
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Description: Precision®FAST |
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Blue dye for adding to 10ml Precision Master Mix |
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| Z-BLUEDYE-10ML | Novacyt Group | 60µl | EUR 69 |
Blue dye for adding to 20ml Precision Master Mix |
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| Z-BLUEDYE-20ML | Novacyt Group | 115µl | EUR 105 |
Multiplex PCR Mastermix (2X) No-ROX |
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| M100-1000 | GeneOn | 1000 rcs (12,5 ml) | EUR 1314 |
Multiplex PCR Mastermix (2X) No-ROX |
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| M100-200 | GeneOn | 200 rcs (2,5 ml) | EUR 329 |
1ml Precision FAST CL & SY qPCR Master Mix |
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| Z-PFAST-CL-SY-1ML | Novacyt Group | 1ml | EUR 90 |
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Description: Precision®FAST |
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2ml Precision FAST CL & SY qPCR Master Mix |
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| Z-PFAST-CL-SY-2ML | Novacyt Group | 2ml | EUR 161 |
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Description: Precision®FAST |
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1ml Precision FAST iC & SY qPCR Master Mix |
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| Z-PFAST-iC-SY-1ML | Novacyt Group | 1ml | EUR 90 |
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Description: Precision®FAST |
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2ml Precision FAST iC & SY qPCR Master Mix |
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| Z-PFAST-iC-SY-2ML | Novacyt Group | 2ml | EUR 161 |
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Description: Precision®FAST |
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1ml Precision FAST LR & SY qPCR Master Mix |
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| Z-PFAST-LR-SY-1ML | Novacyt Group | 1ml | EUR 90 |
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Description: Precision®FAST |
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2ml Precision FAST LR & SY qPCR Master Mix |
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| Z-PFAST-LR-SY-2ML | Novacyt Group | 2ml | EUR 161 |
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Description: Precision®FAST |
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1ml Precision FAST MX & SY qPCR Master Mix |
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| Z-PFAST-MX-SY-1ML | Novacyt Group | 1ml | EUR 90 |
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Description: Precision®FAST |
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2ml Precision FAST MX & SY qPCR Master Mix |
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| Z-PFAST-MX-SY-2ML | Novacyt Group | 2ml | EUR 161 |
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Description: Precision®FAST |
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1ml Precision FAST R & SY qPCR Master Mix |
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| Z-PFAST-R-SY-1ML | Novacyt Group | 1ml | EUR 90 |
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Description: Precision®FAST |
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2ml Precision FAST R & SY qPCR Master Mix |
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| Z-PFAST-R-SY-2ML | Novacyt Group | 2ml | EUR 161 |
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Description: Precision®FAST |
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MegaFi Pro Fidelity 2X PCR MasterMix |
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| G887 | ABM | 800 rxn (10.0 ml) | EUR 325 |
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Description: MegaFi™ Fidelity 2X PCR MasterMix sets new standards for sensitive, robust, and high-fidelity PCR performance. This strategically-engineered polymerase has exceptional sensitivity and can amplify even the most difficult templates (compared to the leading competitors). In addition, MegaFi™ has high fidelity and ultra-low error rates (over 1,000X less than Taq polymerase, representing the lowest error rate on the market), making it incredibly useful for a variety of PCR applications demanding high fidelity, including Next Generation Sequencing, molecular cloning, or diagnostics.Key Features• Our polymerase is strategically-engineered for fast versatile high fidelity PCR. • Suitable for long range PCR up to 18 kb from less difficult targets or up to 15 kb from genomic DNA. |
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Multiplex PCR Mastermix (2X) Low-ROX |
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| M110-1000 | GeneOn | 1000 rcs (12,5 ml) | EUR 1314 |
Multiplex PCR Mastermix (2X) Low-ROX |
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| M110-200 | GeneOn | 200 rcs (2,5 ml) | EUR 329 |
MegaFi Pro Fidelity 2X PCR MasterMix |
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| P887-1 | ABM | 100 rxn (1.25 ml) | EUR 95 |
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Description: MegaFi™ Fidelity 2X PCR MasterMix sets new standards for sensitive, robust, and high-fidelity PCR performance. This strategically-engineered polymerase has exceptional sensitivity and can amplify even the most difficult templates (compared to the leading competitors). In addition, MegaFi™ has high fidelity and ultra-low error rates (over 1,000X less than Taq polymerase, representing the lowest error rate on the market), making it incredibly useful for a variety of PCR applications demanding high fidelity, including Next Generation Sequencing, molecular cloning, or diagnostics.Key Features• Our polymerase is strategically-engineered for fast versatile high fidelity PCR. • Suitable for long range PCR up to 18 kb from less difficult targets or up to 15 kb from genomic DNA. |
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Multiplex PCR Mastermix (2X) High-ROX |
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| M120-1000 | GeneOn | 1000 rcs (12,5 ml) | Ask for price |
Multiplex PCR Mastermix (2X) High-ROX |
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| M120-200 | GeneOn | 200 rcs (2,5 ml) | Ask for price |
Hi-Quanti 2X Realtime PCR Mastermix |
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| MBT180-100R | EWC Diagnostics | 1 unit | EUR 46.42 |
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Description: Hi-Quanti 2X Realtime PCR Mastermix |
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Hi-Quanti 2X Realtime PCR Mastermix |
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| MBT180-20R | EWC Diagnostics | 1 unit | EUR 11.74 |
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Description: Hi-Quanti 2X Realtime PCR Mastermix |
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Hi-Quanti 2X Realtime PCR Mastermix |
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| MBT180-50R | EWC Diagnostics | 1 unit | EUR 25.79 |
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Description: Hi-Quanti 2X Realtime PCR Mastermix |
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EzWay Direct Multiplex PCR MasterMix (2x) |
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| K0567840 | KOMA BIOTECH | 1ml | EUR 161 |
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Description: https://komabiotech.co.kr/labiskoma/datasheet/K0568010,K0568020,K0568210,K0569020,K0567840.pdf |
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1ml Precision FAST qPCR Master Mix with ROX |
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| Z-PFAST-R-1ML | Novacyt Group | 1ml | EUR 90 |
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Description: Precision®FAST |
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2ml Precision FAST qPCR Master Mix with ROX |
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| Z-PFAST-R-2ML | Novacyt Group | 2ml | EUR 161 |
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Description: Precision®FAST |
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5ml Precision FAST qPCR Master Mix (5 x 1ml) |
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| Z-PFAST-5ML | Novacyt Group | 5ml | EUR 348 |
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Description: Precision®FAST |
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2X PCR Taq Plus MasterMix with dye |
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| G901-dye | ABM | 25.0 ml (1000 Rxns) | EUR 358.8 |
2X PCR Taq Plus MasterMix with dye |
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| G014-dye | ABM | 5.0 ml (200 Rxns) | EUR 127.2 |
10ml Precision FAST qPCR Master Mix (10 x 1ml) |
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| Z-PFAST-10ML | Novacyt Group | 10ml | EUR 641 |
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Description: Precision®FAST |
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20ml Precision FAST qPCR Master Mix (20 x 1ml) |
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| Z-PFAST-20ML | Novacyt Group | 20ml | EUR 1120 |
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Description: Precision®FAST |
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5ml Precision FAST CL qPCR Master Mix (5 x 1ml) |
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| Z-PFAST-CL-5ML | Novacyt Group | 5ml | EUR 348 |
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Description: Precision®FAST |
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5ml Precision FAST iC qPCR Master Mix (5 x 1ml) |
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| Z-PFAST-iC-5ML | Novacyt Group | 5ml | EUR 348 |
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Description: Precision®FAST |
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5ml Precision FAST LR qPCR Master Mix (5 x 1ml) |
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| Z-PFAST-LR-5ML | Novacyt Group | 5ml | EUR 348 |
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Description: Precision®FAST |
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5ml Precision FAST MX qPCR Master Mix (5 x 1ml) |
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| Z-PFAST-MX-5ML | Novacyt Group | 5ml | EUR 348 |
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Description: Precision®FAST |
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5ml Precision FAST SY qPCR Master Mix (5 x 1ml) |
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| Z-PFAST-SY-5ML | Novacyt Group | 5ml | EUR 348 |
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Description: Precision®FAST |
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10ml Precision FAST CL qPCR Master Mix (10 x 1ml) |
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| Z-PFAST-CL-10ML | Novacyt Group | 10ml | EUR 641 |
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Description: Precision®FAST |
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20ml Precision FAST CL qPCR Master Mix(20 x 1ml) |
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| Z-PFAST-CL-20ML | Novacyt Group | 20ml | EUR 1120 |
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Description: Precision®FAST |
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10ml Precision FAST iC qPCR Master Mix (10 x 1ml) |
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| Z-PFAST-iC-10ML | Novacyt Group | 10ml | EUR 641 |
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Description: Precision®FAST |
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20ml Precision FAST iC qPCR Master Mix (20 x 1ml) |
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| Z-PFAST-iC-20ML | Novacyt Group | 20ml | EUR 1120 |
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Description: Precision®FAST |
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10ml Precision FAST LR qPCR Master Mix (10 x 1ml) |
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| Z-PFAST-LR-10ML | Novacyt Group | 10ml | EUR 641 |
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Description: Precision®FAST |
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20ml Precision FAST LR qPCR Master Mix (20 x 1ml) |
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| Z-PFAST-LR-20ML | Novacyt Group | 20ml | EUR 1120 |
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Description: Precision®FAST |
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10ml Precision FAST MX qPCR Master Mix (10 x 1ml) |
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| Z-PFAST-MX-10ML | Novacyt Group | 10ml | EUR 641 |
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Description: Precision®FAST |
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20ml Precision FAST MX qPCR Master Mix (20 x 1ml) |
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| Z-PFAST-MX-20ML | Novacyt Group | 20ml | EUR 1120 |
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Description: Precision®FAST |
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10ml Precision FAST SY qPCR Master Mix (10 x 1ml) |
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| Z-PFAST-SY-10ML | Novacyt Group | 10ml | EUR 641 |
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Description: Precision®FAST |
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20ml Precision FAST SY qPCR Master Mix (20 x 1ml) |
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| Z-PFAST-SY-20ML | Novacyt Group | 20ml | EUR 1120 |
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Description: Precision®FAST |
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2X PCR Bestaq™ MasterMix with dye |
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| G464-dye | ABM | 5.0 ml (200 Rxns) | EUR 189.6 |
5ml Precision FAST CL & SY qPCR Master Mix (5 x 1ml) |
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| Z-PFAST-CL-SY-5ML | Novacyt Group | 5ml | EUR 348 |
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Description: Precision®FAST |
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5ml Precision FAST iC & SY qPCR Master Mix (5 x 1ml) |
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| Z-PFAST-iC-SY-5ML | Novacyt Group | 5ml | EUR 348 |
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Description: Precision®FAST |
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5ml Precision FAST LR & SY qPCR Master Mix (5 x 1ml) |
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| Z-PFAST-LR-SY-5ML | Novacyt Group | 5ml | EUR 348 |
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Description: Precision®FAST |
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5ml Precision FAST MX & SY qPCR Master Mix (5 x 1ml) |
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| Z-PFAST-MX-SY-5ML | Novacyt Group | 5ml | EUR 348 |
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Description: Precision®FAST |
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5ml Precision FAST R & SY qPCR Master Mix (5 x 1ml) |
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| Z-PFAST-R-SY-5ML | Novacyt Group | 5ml | EUR 348 |
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Description: Precision®FAST |
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Safe-Green™ 2X PCR Taq MasterMix |
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| G472 | ABM | 5.0 ml (200 Rxns) | EUR 127.2 |
10ml Precision FAST CL & SY qPCR Master Mix (10 x 1ml) |
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| Z-PFAST-CL-SY-10ML | Novacyt Group | 10ml | EUR 641 |
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Description: Precision®FAST |
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20ml Precision FAST CL & SY qPCR Master Mix (20 x 1ml) |
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| Z-PFAST-CL-SY-20ML | Novacyt Group | 20ml | EUR 1120 |
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Description: Precision®FAST |
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10ml Precision FAST iC & SY qPCR Master Mix (10 x 1ml) |
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| Z-PFAST-iC-SY-10ML | Novacyt Group | 10ml | EUR 641 |
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Description: Precision®FAST |
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20ml Precision FAST iC & SY qPCR Master Mix (20 x 1ml) |
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| Z-PFAST-iC-SY-20ML | Novacyt Group | 20ml | EUR 1120 |
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Description: Precision®FAST |
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10ml Precision FAST LR & SY qPCR Master Mix (10 x 1ml) |
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| Z-PFAST-LR-SY-10ML | Novacyt Group | 10ml | EUR 641 |
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Description: Precision®FAST |
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20ml Precision FAST LR & SY qPCR Master Mix (20 x 1ml) |
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| Z-PFAST-LR-SY-20ML | Novacyt Group | 20ml | EUR 1120 |
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Description: Precision®FAST |
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10ml Precision FAST MX & SY qPCR Master Mix (10 x 1ml) |
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| Z-PFAST-MX-SY-10ML | Novacyt Group | 10ml | EUR 641 |
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Description: Precision®FAST |
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20ml Precision FAST MX & SY qPCR Master Mix (20 x 1ml) |
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| Z-PFAST-MX-SY-20ML | Novacyt Group | 20ml | EUR 1120 |
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Description: Precision®FAST |
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10ml Precision FAST R & SY qPCR Master Mix (10 x 1ml) |
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| Z-PFAST-R-SY-10ML | Novacyt Group | 10ml | EUR 641 |
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Description: Precision®FAST |
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20ml Precision FAST R & SY qPCR Master Mix (20 x 1ml) |
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| Z-PFAST-R-SY-20ML | Novacyt Group | 20ml | EUR 1120 |
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Description: Precision®FAST |
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2X PCR SensTaq HotStart MasterMix with Dye |
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| G939-Dye | ABM | 5.0 ml (200 Rxns) | EUR 189.6 |
5ml Precision FAST qPCR Master Mix with ROX (5 x 1ml) |
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| Z-PFAST-R-5ML | Novacyt Group | 5ml | EUR 348 |
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Description: Precision®FAST |
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10ml Precision FAST qPCR Master Mix with ROX (10 x 1ml) |
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| Z-PFAST-R-10ML | Novacyt Group | 10ml | EUR 641 |
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Description: Precision®FAST |
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20ml Precision FAST qPCR Master Mix with ROX (20 x 1ml) |
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| Z-PFAST-R-20ML | Novacyt Group | 20ml | EUR 1120 |
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Description: Precision®FAST |
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1ml Precision FAST qPCR Master Mix for BioRad iCycler |
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| Z-PFAST-iC-1ML | Novacyt Group | 1ml | EUR 90 |
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Description: Precision®FAST |
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2ml Precision FAST qPCR Master Mix for BioRad iCycler |
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| Z-PFAST-iC-2ML | Novacyt Group | 2ml | EUR 161 |
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Description: Precision®FAST |
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1ml Precision FAST qPCR Master Mix premixed with SYBRgreen |
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| Z-PFAST-SY-1ML | Novacyt Group | 1ml | EUR 90 |
|
Description: Precision®FAST |
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2ml Precision FAST qPCR Master Mix premixed with SYBRgreen |
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| Z-PFAST-SY-2ML | Novacyt Group | 2ml | EUR 161 |
|
Description: Precision®FAST |
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1ml Precision FAST qPCR Master Mix for Stratagene hardware |
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| Z-PFAST-MX-1ML | Novacyt Group | 1ml | EUR 90 |
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Description: Precision®FAST |
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2ml Precision FAST qPCR Master Mix for Stratagene hardware |
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| Z-PFAST-MX-2ML | Novacyt Group | 2ml | EUR 161 |
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Description: Precision®FAST |
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Safe-Green™ 2X PCR Bestaq™ MasterMix |
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| G478 | ABM | 5.0 ml (200 Rxns) | EUR 212.4 |
EzWay Direct Taq PCR MasterMix w/o dye (2x) |
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| K0568020 | KOMA BIOTECH | 1ml | EUR 108 |
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Description: https://komabiotech.co.kr/labiskoma/datasheet/K0568010,K0568020,K0568210,K0569020,K0567840.pdf |
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BlasTaq 2X qPCR MasterMix |
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| G891 | Applied Biological Materials France | 500 rxn (4 x 1.25 ml) | EUR 135 |
|
Description: BlasTaq™ 2X qPCR MasterMix provides a convenient, reliable and robust setup for performing quantitative real-time analysis of DNA samples. This ready-to-use qPCR MasterMix contains abm’s strategically-engineered, next generation Taq Polymerase, BlasTaq™ DNA Polymerase, providing for rapid extension rates and robust performance. With specialized reaction conditions, this polymerase provides increased processivity, yields, and sensitivity, while shortening reaction times by up to 70%, compared to wild-type Taq DNA polymerase. ROX reference dye is provided separate from the MasterMix, making this kit universally compatible with most qPCR instruments.BlasTaq™ has 5’-3’ polymerase and 5’-3’ exonuclease activities, lacks 3’-5’ exonuclease activity, and produces 3’-dA-tailed amplicons. qPCR products made with BlasTaq™ can be used with TA cloning vectors.Key Features• Enable streamlined protocol in a simple reaction set-up. • Allow accurate quantification of a variety of gene targets. • Reduce pipetting steps to minimize the risk of contamination.• Compatible with most real-time PCR instruments. |
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BlasTaq 2X qPCR MasterMix |
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| G891-S | ABM | 125 rxn (1 x 1.25 ml) | EUR 60 |
|
Description: BlasTaq™ 2X qPCR MasterMix provides a convenient, reliable and robust setup for performing quantitative real-time analysis of DNA samples. This ready-to-use qPCR MasterMix contains abm’s strategically-engineered, next generation Taq Polymerase, BlasTaq™ DNA Polymerase, providing for rapid extension rates and robust performance. With specialized reaction conditions, this polymerase provides increased processivity, yields, and sensitivity, while shortening reaction times by up to 70%, compared to wild-type Taq DNA polymerase. ROX reference dye is provided separate from the MasterMix, making this kit universally compatible with most qPCR instruments.BlasTaq™ has 5’-3’ polymerase and 5’-3’ exonuclease activities, lacks 3’-5’ exonuclease activity, and produces 3’-dA-tailed amplicons. qPCR products made with BlasTaq™ can be used with TA cloning vectors.Key Features• Enable streamlined protocol in a simple reaction set-up. • Allow accurate quantification of a variety of gene targets. • Reduce pipetting steps to minimize the risk of contamination.• Compatible with most real-time PCR instruments. |
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BlasTaq 2X qPCR MasterMix |
|||
| G892 | Applied Biological Materials France | 2,500 rxn (25.0 ml) | EUR 545 |
|
Description: BlasTaq™ 2X qPCR MasterMix provides a convenient, reliable and robust setup for performing quantitative real-time analysis of DNA samples. This ready-to-use qPCR MasterMix contains abm’s strategically-engineered, next generation Taq Polymerase, BlasTaq™ DNA Polymerase, providing for rapid extension rates and robust performance. With specialized reaction conditions, this polymerase provides increased processivity, yields, and sensitivity, while shortening reaction times by up to 70%, compared to wild-type Taq DNA polymerase. ROX reference dye is provided separate from the MasterMix, making this kit universally compatible with most qPCR instruments.BlasTaq™ has 5’-3’ polymerase and 5’-3’ exonuclease activities, lacks 3’-5’ exonuclease activity, and produces 3’-dA-tailed amplicons. qPCR products made with BlasTaq™ can be used with TA cloning vectors.Key Features• Enable streamlined protocol in a simple reaction set-up. • Allow accurate quantification of a variety of gene targets. • Reduce pipetting steps to minimize the risk of contamination.• Compatible with most real-time PCR instruments. |
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Disaster preparedness to maintain sample and reagent integrity
- Freezers and LN2 tanks at biomolecular facilities often store irreplaceable samples and expensive reagents that must be maintained at the correct storage conditions. Temperature excursions from the recommended storage conditions may cause sample degradation and reduced reagent activity, making them unusable. Implementing a disaster preparedness plan including a robust monitoring system reduces the risk of losing samples and reagents due to natural disaster, HVAC malfunction, freezer/dewar failure and power loss.
- Cold storage devices should be located in secure locations which are unlikely to be affected by naturally occurring events including floods or hurricanes. Freezers are designed to operate in a specific temperature range and monitoring the room temperature will reduce the likelihood of devices operating outside of the recommended temperature range due to HVAC failures.
- Optimally, freezers and the monitoring system will be connected to a reliable emergency power system. Battery backup systems are available for cold storage devices and the monitoring system. Power loss can be mitigated with dry ice or a CO2/LN2 backup system. Access to the appropriate number of backup freezers or LN2 dewars ensures uninterrupted storage at the correct temperature when the primary storage device is inoperable.
- Continuous monitoring of temperature, power and the network functionality is critical to ensure the cold storage devices are operating correctly and that active alarms receive the appropriate attention. An institutional network can be augmented with additional communication devices providing an alternate path for reporting and alert notifications. Losing irreplaceable samples and reagents can be devastating. Advanced planning for natural disasters, equipment failures and power outages enables a fast, streamlined response and ensures the integrity of samples and reagents.
Long-term dry storage of enzyme-based reagents for isothermal nucleic acid amplification in a porous matrix for use in point-of-care diagnostic devices
Nucleic acid amplification test (NAAT)-based point-of-care (POC) devices are rapidly growing for use in low-resource settings. However, key challenges are the ability to store the enzyme-based reagents in dry form in the device and the long-term stability of those reagents at elevated temperatures, especially where ambient temperatures could be as high as 45 °C. Here, we describe a set of excipients including a combination of trehalose, polyethylene glycol and dextran, and a method for using them that allows long-term dry storage of enzyme-based reagents for an isothermal strand displacement amplification (iSDA) reaction in a porous matrix.
Various porous materials, including nitrocellulose, cellulose, and glass fiber, were tested. Co-dried reagents for iSDA always included those that amplified the ldh1 gene in Staphylococcus aureus (a polymerase and a nicking enzyme, 4 primers, dNTPs and a buffer). Reagents also either included a capture probe and a streptavidin-Au label required for lateral flow (LF) detection after amplification, or a fluorescent probe used for real-time detection.
The reagents showed the best stability in a glass fiber matrix when stored in the presence of 10% trehalose and 2.5% dextran. The reagents were stable for over a year at ∼22 °C as determined by lateral flow detection and gel electrophoresis. The reagents also exhibited excellent stability after 360 h at 45 °C; the assay still detected as few as 10 copies of ldh1 gene target by lateral flow detection, and 50 copies with real-time fluorescence detection. These results demonstrate the potential for incorporation of amplification reagents in dry form in point-of-care devices for use in a wide range of settings.
HiScript One Step RT-PCR Kit |
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| MBT095-10R | EWC Diagnostics | 1 unit | EUR 61.34 |
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Description: HiScript One Step RT-PCR Kit |
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HiScript One Step RT-PCR Kit |
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| MBT095-25R | EWC Diagnostics | 1 unit | EUR 141.96 |
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Description: HiScript One Step RT-PCR Kit |
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HiScript One Step RT-PCR Kit |
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| MBT095-50R | EWC Diagnostics | 1 unit | EUR 247.73 |
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Description: HiScript One Step RT-PCR Kit |
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One Step M-MULV RT-PCR Kit |
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| BS665 | Bio Basic | 100preps | EUR 477.6 |
ONE STEP RT-PCR REAL TIME KIT |
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| MBT128-10R | EWC Diagnostics | 1 unit | EUR 37.4 |
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Description: ONE STEP RT-PCR REAL TIME KIT |
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ONE STEP RT-PCR REAL TIME KIT |
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| MBT128-25R | EWC Diagnostics | 1 unit | EUR 89.72 |
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Description: ONE STEP RT-PCR REAL TIME KIT |
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ONE STEP RT-PCR REAL TIME KIT |
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| MBT128-2X50R | EWC Diagnostics | 1 unit | EUR 314.51 |
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Description: ONE STEP RT-PCR REAL TIME KIT |
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ONE STEP RT-PCR REAL TIME KIT |
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| MBT128-50R | EWC Diagnostics | 1 unit | EUR 165.56 |
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Description: ONE STEP RT-PCR REAL TIME KIT |
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HiScript II One Step RT-PCR Kit |
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| P611-01 | Vazyme | 50 rxn (50 μl/rxn) | EUR 108.5 |
One Step Probe RT-PCR Mix |
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| EQ015 | ELK Biotech | 20 µL x 100 rxn | EUR 248 |
One Step Probe RT-PCR Mix |
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| EQ015-20Lx100rxn | ELK Biotech | 20µLx100rxn | EUR 80 |
One Step Probe RT-PCR Mix |
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| EQ015-20Lx500rxn | ELK Biotech | 20µLx500rxn | EUR 248 |
BioRT Master One Step RT-PCR Kit Ⅲ |
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| TRI-B72S1 | TRI Biotech | 100T | EUR 425.88 |
One Step SYBR Green RT-PCR Mix |
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| EQ007 | ELK Biotech | 20 µL x 100 rxn | EUR 248 |
One Step SYBR Green RT-PCR Mix |
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| EQ007-20Lx100rxn | ELK Biotech | 20µLx100rxn | EUR 80 |
One Step SYBR Green RT-PCR Mix |
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| EQ007-20Lx500rxn | ELK Biotech | 20µLx500rxn | EUR 248 |
HiScript II One Step RT-PCR Kit (Dye Plus) |
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| P612-01 | Vazyme | 50 rxn (50 μl/rxn) | EUR 108.5 |
igScript™ One Step RT-PCR Kit - 100 Reactions |
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| 4211 | Intact Genomics | 1/EA | EUR 180 |
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Description: igScript™ One Step RT-PCR kit combines two powerful mixtures: i) igScript™ Reverse Transcriptase and ii) Hot start Taq 2x master mix providing improved PCR efficiency, wider dynamic range, superior sensitivity and specificity. The two mixtures require minimal handling during reaction setup and offer consistent and robust RT-PCR reactions.Product Includes:ig Script™ Reverse TranscriptaseHot start Taq 2x Master Mix |
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igScript™ One Step RT-PCR Kit - 500 Reactions |
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| 4213 | Intact Genomics | 1/EA | EUR 680 |
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Description: igScript™ One Step RT-PCR kit combines two powerful mixtures: i) igScript™ Reverse Transcriptase and ii) Hot start Taq 2x master mix providing improved PCR efficiency, wider dynamic range, superior sensitivity and specificity. The two mixtures require minimal handling during reaction setup and offer consistent and robust RT-PCR reactions.Product Includes:ig Script™ Reverse TranscriptaseHot start Taq 2x Master Mix |
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Easy? One Step RT Kit |
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| M1162-100 | Biovision | each | EUR 561.6 |
Hi-Quanti one step probe based RT-PCR |
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| MBT181-10R | EWC Diagnostics | 1 unit | EUR 61.34 |
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Description: Hi-Quanti one step probe based RT-PCR |
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Hi-Quanti one step probe based RT-PCR |
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| MBT181-25R | EWC Diagnostics | 1 unit | EUR 141.93 |
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Description: Hi-Quanti one step probe based RT-PCR |
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Hi-Quanti one step probe based RT-PCR |
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| MBT181-50R | EWC Diagnostics | 1 unit | EUR 247.73 |
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Description: Hi-Quanti one step probe based RT-PCR |
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BioRT Master One Step RT-PCR Kit II (with dye) |
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| TRI-B57M1 | TRI Biotech | 100T | EUR 498.96 |
BioRT Master One Step RT-PCR Kit Ⅲ (with dye) |
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| TRI-B72L1 | TRI Biotech | 5000T | EUR 18592.56 |
BioRT Master One Step RT-PCR Kit Ⅲ (with dye) |
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| TRI-B72M1 | TRI Biotech | 500T | EUR 1993.32 |
One-Step RT-PCR Kit |
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| G174 | ABM | 100 x 50 ul reactions | EUR 279.6 |
Easy? One Step RT Kit-Dye |
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| M1163-100 | Biovision | each | EUR 561.6 |
One Step RT-qPCR Probe Kit |
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| MBS9141941-100Reactions | MyBiosource | 100Reactions | EUR 300 |
One Step RT-qPCR Probe Kit |
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| MBS9141941-20Reactions | MyBiosource | 20Reactions | EUR 190 |
One Step RT-qPCR Probe Kit |
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| MBS9141941-5x100Reactions | MyBiosource | 5x100Reactions | EUR 1280 |
One Step MultiPlex qRT PCR Kit with Probe |
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| 9K-005-0012 | Bio Basic | 100Rxn, 100prep | EUR 372.16 |
UltraSYBR One Step RT-qPCR Kit |
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| MBS278015-100Reactions | MyBiosource | 100Reactions | EUR 275 |
Taq One-Step RT-PCR Kit |
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| abx461044-1096tests | Abbexa | 10 × 96 tests | Ask for price |
Taq One-Step RT-PCR Kit |
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| abx461044-596tests | Abbexa | 5 × 96 tests | EUR 962.5 |
Taq One-Step RT-PCR Kit |
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| abx461044-96tests | Abbexa | 96 tests | EUR 475 |
MyTaq One-Step RT-PCR Kit |
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| BIO-65048 | Bioline | 25 Reactions | Ask for price |
MyTaq One-Step RT-PCR Kit |
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| BIO-65049 | Bioline | 100 Reactions | Ask for price |
MegaFi One-Step RT-PCR Kit |
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| G597 | ABM | 100 rxn | EUR 215 |
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Description: MegaFi™ One-Step RT-PCR is an enzyme mix of OneScript® Hot Reverse Transcriptase and MegaFi™ Fidelity DNA Polymerase with RNaseOFF Ribonuclease Inhibitor, gel loading dye, and all other necessary reagents in a single One-Step 2X RT-PCR Buffer for both highly sensitive and specific reverse transcription and high fidelity PCR amplification in a single reaction tube from any RNA template. The kit provides flexibility in choosing desired primers for use with a proprietary RT-PCR buffer containing stabilizers and enhancers that optimize the two reactions in a “single step”. This kit offers simple, efficient reaction setup, and is a reliable alternative to conventional “two-step” sequential RT-PCR. |
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MegaFi One-Step RT-PCR Kit |
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| E36G597 | EnoGene | 100 rxn | EUR 271.43 |
MegaFi One-Step RT-PCR Kit |
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| MBS8580085-100Reactions | MyBiosource | 100Reactions | EUR 365 |
MegaFi One-Step RT-PCR Kit |
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| MBS8580085-5x100Reactions | MyBiosource | 5x100Reactions | EUR 1660 |
igScript™ One Step RT-qPCR Kit |
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| 4214 | Intact Genomics | 1/EA | EUR 156 |
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Description: igScript™ one step RT-qPCR kit combines two powerful mixtures: i). igScript™ Reverse Transcriptase and ii) ig SYBR® Green qPCR 2x master mix with standard buffer providing improved PCR efficiency, wider dynamic range, superior sensitivity and specificity. The two mixtures require minimal handling during reaction setup and offer consistent and robust RT-qPCR reactions.Product Includes:ig Script™ Reverse Transcriptaseig SYBR™ Green qPCR 2x Master Mix |
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HiScript Two Step RT-PCR Kit |
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| MBT138-10R | EWC Diagnostics | 1 unit | EUR 78.64 |
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Description: HiScript Two Step RT-PCR Kit |
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HiScript Two Step RT-PCR Kit |
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| MBT138-25R | EWC Diagnostics | 1 unit | EUR 180.73 |
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Description: HiScript Two Step RT-PCR Kit |
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HiScript Two Step RT-PCR Kit |
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| MBT138-50R | EWC Diagnostics | 1 unit | EUR 325.31 |
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Description: HiScript Two Step RT-PCR Kit |
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amfiRivert 1-Step RT-PCR Kit |
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| R5003-050 | GenDepot | 50 rxns | Ask for price |
amfiRivert 1-Step RT-PCR Kit |
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| R5003-100 | GenDepot | 100 rxns | Ask for price |
amfiRivert 1-Step RT-PCR Kit |
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| R5003-200 | GenDepot | 200 rxns | Ask for price |
One Step qRT-PCR Kit(一步法荧光定量反转录PCR Kit) |
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| 4305A | Unibiotest | 50 Tests*20μL | EUR 100 |
One Step qRT-PCR Kit(一步法荧光定量反转录PCR Kit) |
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| 4305B | Unibiotest | 125 Tests*20μL | EUR 200 |
One Step qRT-PCR Kit(一步法荧光定量反转录PCR Kit) |
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| 4305C | Unibiotest | 250 Tests*20μL | EUR 350 |
One-Step RT-PCR Kit with dye |
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| G174-dye | ABM | 100 x 50 ul reactions | EUR 279.6 |
ABScript II One Step RT-qPCR Probe Kit |
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| RK20407 | Abclonal | 100RXN | EUR 21.26 |
EzWay One-Step RT-PCR MasterMix |
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| K0569600 | KOMA BIOTECH | 1ml | EUR 244 |
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Description: https://komabiotech.co.kr/labiskoma/datasheet/K0569600.pdf |
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HiScript II One Step qRT-PCR Probe Kit |
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| Q222-01 | Vazyme | 100 rxn (50 μl/rxn) | EUR 133.5 |
HiScript II One Step qRT-PCR Probe Kit |
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| Q223-EN01 | Vazyme | 250 rxns (20 μl/rxn) | EUR 157 |
HiScript II One Step qRT-PCR Probe Kit |
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| Q223-EN01-250rxns | Vazyme | 250 rxns | EUR 162.57 |
HiScript III One Step qRT-PCR Probe Kit |
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| Q225-EN01 | Vazyme | 100 rxns | EUR 94.21 |
HiScript III One Step qRT-PCR Probe Kit |
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| Q225-EN01-100rxns | Vazyme | 100 rxns | EUR 97.55 |
HiScript III One Step qRT-PCR Probe Kit |
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| Q225-EN02 | Vazyme | 1,000 rxns | EUR 710.17 |
HiScript III One Step qRT-PCR Probe Kit |
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| Q225-EN02-1000rxns | Vazyme | 1,000 rxns | EUR 735.38 |
HiScript III One Step qRT-PCR Probe Kit |
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| Q225-EN03 | Vazyme | 5,000 rxns | EUR 3261 |
HiScript III One Step qRT-PCR Probe Kit |
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| Q225-EN03-5000rxns | Vazyme | 5,000 rxns | EUR 3376.77 |
ABScript II One Step SYBR Green RT-qPCR Kit |
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| RK20404 | Abclonal | 10 RXN | EUR 119.9 |
Sapovirus One-Step PCR kit |
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| Oneq-H630-100R | Bioingentech | 100T | EUR 1932 |
Sapovirus One-Step PCR kit |
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| Oneq-H630-150R | Bioingentech | 150T | EUR 2646 |
Sapovirus One-Step PCR kit |
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| Oneq-H630-50R | Bioingentech | 50T | EUR 1410 |
Entamoeba One-Step PCR kit |
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| Oneq-H683-100D | Bioingentech | 100T | EUR 1039.2 |
Entamoeba One-Step PCR kit |
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| Oneq-H683-150D | Bioingentech | 150T | EUR 1177.2 |
Entamoeba One-Step PCR kit |
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| Oneq-H683-50D | Bioingentech | 50T | EUR 861.6 |
Echovirus One-Step PCR kit |
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| Oneq-H722-100D | Bioingentech | 100T | EUR 1039.2 |
Echovirus One-Step PCR kit |
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| Oneq-H722-150D | Bioingentech | 150T | EUR 1177.2 |
Echovirus One-Step PCR kit |
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| Oneq-H722-50D | Bioingentech | 50T | EUR 861.6 |
ranavirus One-Step PCR kit |
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| Oneq-V337-100D | Bioingentech | 100T | EUR 1039.2 |
ranavirus One-Step PCR kit |
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| Oneq-V337-150D | Bioingentech | 150T | EUR 1177.2 |
ranavirus One-Step PCR kit |
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| Oneq-V337-50D | Bioingentech | 50T | EUR 861.6 |
Mengovirus One-Step PCR kit |
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| Oneq-H453-100R | Bioingentech | 100T | EUR 1932 |
Mengovirus One-Step PCR kit |
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| Oneq-H453-150R | Bioingentech | 150T | EUR 2646 |
Mengovirus One-Step PCR kit |
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| Oneq-H453-50R | Bioingentech | 50T | EUR 1410 |
Astrovirus One-Step PCR kit |
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| Oneq-H491-100R | Bioingentech | 100T | EUR 1932 |
Astrovirus One-Step PCR kit |
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| Oneq-H491-150R | Bioingentech | 150T | EUR 2646 |
Astrovirus One-Step PCR kit |
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| Oneq-H491-50R | Bioingentech | 50T | EUR 1410 |
Adenovirus One-Step PCR kit |
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| Oneq-H501-100D | Bioingentech | 100T | EUR 1039.2 |
Adenovirus One-Step PCR kit |
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| Oneq-H501-150D | Bioingentech | 150T | EUR 1177.2 |
Adenovirus One-Step PCR kit |
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| Oneq-H501-50D | Bioingentech | 50T | EUR 861.6 |
igScript™ Probe-Based One Step RT-qPCR Kit |
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| 4212 | Intact Genomics | 200 | EUR 156 |
|
Description: IgScript™ Probe-Based One Step RT-qPCR 2x master mix contains IgScript™ Reverse Transcriptase, Taq DNA polymerase, MgCl2, dNTPs, stabilizers and low ROX reference dye with standard buffer providing improved RT-qPCR efficiency, wider dynamic range, superior sensitivity and specificity. IgScript™ Probe-Based One Step RT-qPCR 2x master mix is a ready-to-use cocktail containing all components except primers, probe and template, for the amplification and detection of DNA in RT-qPCR. This 2x master mix requires minimal handling during reaction setup and offer consistent and robust RT-qPCR reactions.IgScript™ Reverse Transcriptase is a recombinant MMLV reverse transcriptase with reduced RNase H activity, increased thermostability and can produce cDNA from small amount of total RNA for real-time RT-qPCR analysis and other applications. Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→3´ polymerase (1, 2) and a 5´→3´ exonuclease activity (3, 4). The amplification step features a high quality Taq DNA Polymerase which offers robust, reliable and better amplification.Product IncludesIgScript one step RT-qPCR 2x master mixApplicationsGene expression data validation.MultiplexingMutation detectionPathogen and viral detectionGenetically modified organisms (GMO) characterization and Genetic profiling |
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igScript™ Probe-Based One Step RT-qPCR Kit |
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| 4215 | Intact Genomics | 500 | EUR 340 |
|
Description: IgScript™ Probe-Based One Step RT-qPCR 2x master mix contains IgScript™ Reverse Transcriptase, Taq DNA polymerase, MgCl2, dNTPs, stabilizers and low ROX reference dye with standard buffer providing improved RT-qPCR efficiency, wider dynamic range, superior sensitivity and specificity. IgScript™ Probe-Based One Step RT-qPCR 2x master mix is a ready-to-use cocktail containing all components except primers, probe and template, for the amplification and detection of DNA in RT-qPCR. This 2x master mix requires minimal handling during reaction setup and offer consistent and robust RT-qPCR reactions.IgScript™ Reverse Transcriptase is a recombinant MMLV reverse transcriptase with reduced RNase H activity, increased thermostability and can produce cDNA from small amount of total RNA for real-time RT-qPCR analysis and other applications. Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→3´ polymerase (1, 2) and a 5´→3´ exonuclease activity (3, 4). The amplification step features a high quality Taq DNA Polymerase which offers robust, reliable and better amplification.Product IncludesIgScript one step RT-qPCR 2x master mixApplicationsGene expression data validation.MultiplexingMutation detectionPathogen and viral detectionGenetically modified organisms (GMO) characterization and Genetic profiling |
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AccurSTART One Step RT-qPCR Probe Kit (FOR FAST) |
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| Q231-EN01 | Vazyme | 200 rxns | EUR 231.89 |
AccurSTART One Step RT-qPCR Probe Kit (FOR FAST) |
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| Q231-EN01-200rxns | Vazyme | 200 rxns | EUR 240.12 |
AccurSTART One Step RT-qPCR Probe Kit (FOR FAST) |
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| Q231-EN02 | Vazyme | 1,000 rxns | EUR 797.13 |
AccurSTART One Step RT-qPCR Probe Kit (FOR FAST) |
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| Q231-EN02-1000rxns | Vazyme | 1,000 rxns | EUR 825.42 |
AccurSTART One Step RT-qPCR Probe Kit (FOR FAST) |
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| Q231-EN03 | Vazyme | 10,000 rxns | EUR 7464 |
AccurSTART One Step RT-qPCR Probe Kit (FOR FAST) |
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| Q231-EN03-10000rxns | Vazyme | 10,000 rxns | EUR 7728.97 |
Pospiviroid One-Step PCR kit |
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| Oneq-A546-100R | Bioingentech | 100T | EUR 1932 |
Pospiviroid One-Step PCR kit |
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| Oneq-A546-150R | Bioingentech | 150T | EUR 2646 |
Pospiviroid One-Step PCR kit |
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| Oneq-A546-50R | Bioingentech | 50T | EUR 1410 |
Lactococcus One-Step PCR kit |
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| Oneq-A567-100D | Bioingentech | 100T | EUR 1039.2 |
Lactococcus One-Step PCR kit |
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| Oneq-A567-150D | Bioingentech | 150T | EUR 1177.2 |
Lactococcus One-Step PCR kit |
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| Oneq-A567-50D | Bioingentech | 50T | EUR 861.6 |
Leuconostoc One-Step PCR kit |
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| Oneq-A569-100D | Bioingentech | 100T | EUR 1039.2 |
Leuconostoc One-Step PCR kit |
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| Oneq-A569-150D | Bioingentech | 150T | EUR 1177.2 |
Leuconostoc One-Step PCR kit |
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| Oneq-A569-50D | Bioingentech | 50T | EUR 861.6 |
Pediococcus One-Step PCR kit |
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| Oneq-A571-100D | Bioingentech | 100T | EUR 1039.2 |
Pediococcus One-Step PCR kit |
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| Oneq-A571-150D | Bioingentech | 150T | EUR 1177.2 |
Pediococcus One-Step PCR kit |
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| Oneq-A571-50D | Bioingentech | 50T | EUR 861.6 |
Enterovirus One-Step PCR kit |
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| Oneq-H433-100R | Bioingentech | 100T | EUR 1932 |
Enterovirus One-Step PCR kit |
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| Oneq-H433-150R | Bioingentech | 150T | EUR 2646 |
Enterovirus One-Step PCR kit |
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| Oneq-H433-50R | Bioingentech | 50T | EUR 1410 |
Calicivirus One-Step PCR kit |
|||
| Oneq-H493-100R | Bioingentech | 100T | EUR 1932 |
Calicivirus One-Step PCR kit |
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| Oneq-H493-150R | Bioingentech | 150T | EUR 2646 |
Calicivirus One-Step PCR kit |
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| Oneq-H493-50R | Bioingentech | 50T | EUR 1410 |
Halobacillus One-Step PCR kit |
|||
| Oneq-A635-100D | Bioingentech | 100T | EUR 1039.2 |
Halobacillus One-Step PCR kit |
|||
| Oneq-A635-150D | Bioingentech | 150T | EUR 1177.2 |
Halobacillus One-Step PCR kit |
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| Oneq-A635-50D | Bioingentech | 50T | EUR 861.6 |
Polyomavirus One-Step PCR kit |
|||
| Oneq-H515-100D | Bioingentech | 100T | EUR 1039.2 |
Polyomavirus One-Step PCR kit |
|||
| Oneq-H515-150D | Bioingentech | 150T | EUR 1177.2 |
Polyomavirus One-Step PCR kit |
|||
| Oneq-H515-50D | Bioingentech | 50T | EUR 861.6 |
Ruminococcus One-Step PCR kit |
|||
| Oneq-H619-100D | Bioingentech | 100T | EUR 1039.2 |
Ruminococcus One-Step PCR kit |
|||
| Oneq-H619-150D | Bioingentech | 150T | EUR 1177.2 |
Ruminococcus One-Step PCR kit |
|||
| Oneq-H619-50D | Bioingentech | 50T | EUR 861.6 |
Parechovirus One-Step PCR kit |
|||
| Oneq-H642-100R | Bioingentech | 100T | EUR 1932 |
Parechovirus One-Step PCR kit |
|||
| Oneq-H642-150R | Bioingentech | 150T | EUR 2646 |
Parechovirus One-Step PCR kit |
|||
| Oneq-H642-50R | Bioingentech | 50T | EUR 1410 |
Acanthamoeba One-Step PCR kit |
|||
| Oneq-H684-100D | Bioingentech | 100T | EUR 1039.2 |
Acanthamoeba One-Step PCR kit |
|||
| Oneq-H684-150D | Bioingentech | 150T | EUR 1177.2 |
Acanthamoeba One-Step PCR kit |
|||
| Oneq-H684-50D | Bioingentech | 50T | EUR 861.6 |
Ruminococcus One-Step PCR kit |
|||
| Oneq-V619-100D | Bioingentech | 100T | EUR 896.88 |
|
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Ruminococcus. |
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Ruminococcus One-Step PCR kit |
|||
| Oneq-V619-150D | Bioingentech | 150T | EUR 1006.5 |
|
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Ruminococcus. |
|||
Ruminococcus One-Step PCR kit |
|||
| Oneq-V619-50D | Bioingentech | 50T | EUR 754.2 |
|
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Ruminococcus. |
|||
Lactobacillus One-Step PCR kit |
|||
| Oneq-A566-100D | Bioingentech | 100T | EUR 1039.2 |
Lactobacillus One-Step PCR kit |
|||
| Oneq-A566-150D | Bioingentech | 150T | EUR 1177.2 |
Lactobacillus One-Step PCR kit |
|||
| Oneq-A566-50D | Bioingentech | 50T | EUR 861.6 |
Bifidobacterium One-Step PCR kit |
|||
| Oneq-H621-100D | Bioingentech | 100T | EUR 1039.2 |
Bifidobacterium One-Step PCR kit |
|||
| Oneq-H621-150D | Bioingentech | 150T | EUR 1177.2 |
Bifidobacterium One-Step PCR kit |
|||
| Oneq-H621-50D | Bioingentech | 50T | EUR 861.6 |
Metapneumovirus One-Step PCR kit |
|||
| Oneq-H643-100R | Bioingentech | 100T | EUR 1932 |
Metapneumovirus One-Step PCR kit |
|||
| Oneq-H643-150R | Bioingentech | 150T | EUR 2646 |
Metapneumovirus One-Step PCR kit |
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| Oneq-H643-50R | Bioingentech | 50T | EUR 1410 |
Bifidobacterium One-Step PCR kit |
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| Oneq-V621-100D | Bioingentech | 100T | EUR 896.88 |
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Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Bifidobacterium. |
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Bifidobacterium One-Step PCR kit |
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| Oneq-V621-150D | Bioingentech | 150T | EUR 1006.5 |
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Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Bifidobacterium. |
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Bifidobacterium One-Step PCR kit |
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| Oneq-V621-50D | Bioingentech | 50T | EUR 754.2 |
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Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Bifidobacterium. |
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Accuris One-Step RT-PCR kit, 100 reactions |
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| PR1100-100 | Benchmark Scientific | 1 each | EUR 524.89 |
amfiRivert 1-Step RT-PCR Premix |
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| R5004-048 | GenDepot | 48 rxns | Ask for price |
amfiRivert 1-Step RT-PCR Premix |
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| R5004-096 | GenDepot | 96 rxns | Ask for price |
amfiRivert 1-Step RT-PCR Premix |
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| R5004-480 | GenDepot | 5x96 rxns | Ask for price |
OneScriptTwo-Step RT-PCR Kit |
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| G281 | ABM | 25 reactions | EUR 121.2 |
OneScriptTwo-Step RT-PCR Kit |
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| G282 | ABM | 100 reactions | EUR 212.4 |
Accuris One-Step RT-PCR kit, 50 reactions |
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| PR1100-50 | Benchmark Scientific | 1 each | EUR 290.98 |
AccurSTART One Step RT-qPCR Probe Kit (Glycerol-free) |
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| QL227-EN01 | Vazyme | 200 rxns | EUR 225 |
AccurSTART One Step RT-qPCR Probe Kit (Glycerol-free) |
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| QL227-EN01-200rxns | Vazyme | 200 rxns | EUR 233.26 |
AccurSTART One Step RT-qPCR Probe Kit (Glycerol-free) |
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| QL227-EN02 | Vazyme | 1,000 rxns | EUR 782.5 |
AccurSTART One Step RT-qPCR Probe Kit (Glycerol-free) |
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| QL227-EN02-1000rxns | Vazyme | 1,000 rxns | EUR 810.42 |
AccurSTART One Step RT-qPCR Probe Kit (Glycerol-free) |
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| QL227-EN03 | Vazyme | 5,000 rxns | EUR 3681 |
AccurSTART One Step RT-qPCR Probe Kit (Glycerol-free) |
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| QL227-EN03-5000rxns | Vazyme | 5,000 rxns | EUR 3811.73 |
HiScript II U+ One Step qRT-PCR Probe Kit |
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| Q223-01 | Vazyme | 100 rxn (50 μl/rxn) | EUR 444 |
AccurSTART One Step RT-qPCR Super PreMix (ONE TUBE) |
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| Q621-EN01 | Vazyme | 200 rxns (20 μl/rxn) | EUR 260.87 |
AccurSTART One Step RT-qPCR Super PreMix (ONE TUBE) |
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| Q621-EN01-200rxns | Vazyme | 200 rxns | EUR 270.13 |
AccurSTART One Step RT-qPCR Super PreMix (ONE TUBE) |
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| Q621-EN02 | Vazyme | 1,000 rxns (20 μl/rxn) | EUR 1014.48 |
AccurSTART One Step RT-qPCR Super PreMix (ONE TUBE) |
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| Q621-EN02-1000rxns | Vazyme | 1,000 rxns | EUR 1050.49 |
AccurSTART One Step RT-qPCR Super PreMix (ONE TUBE) |
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| Q621-EN03 | Vazyme | 10,000 rxns (20 μl/rxn) | EUR 8695.5 |
AccurSTART One Step RT-qPCR Super PreMix (ONE TUBE) |
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| Q621-EN03-10000rxns | Vazyme | 10,000 rxns | EUR 9004.19 |
HiScript II One Step qRT-PCR SYBR Green Kit |
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| Q221-00-20rxns | Vazyme | 20 rxns | EUR 1.09 |
HiScript II One Step qRT-PCR SYBR Green Kit |
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| Q221-01 | Vazyme | 100 rxn (50 μl/rxn) | EUR 133.5 |
HiScript II One Step qRT-PCR SYBR Green Kit |
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| Q221-01-250rxns | Vazyme | 250 rxns | EUR 138.24 |
2X One-Step RT-PCR Master Mix |
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| 28113 | Norgen Biotek Corp | 100 Reactions | EUR 161.4 |
2X One-Step RT-PCR Master Mix |
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| 28114 | Norgen Biotek Corp | 200 Reactions | EUR 287.4 |
2X One-Step RT-PCR Master Mix |
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| 28115 | Norgen Biotek Corp | 500 Reactions | EUR 629.4 |
Parvovirus В19 One-Step PCR kit |
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| Oneq-H455-100D | Bioingentech | 100T | EUR 1039.2 |
Parvovirus В19 One-Step PCR kit |
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| Oneq-H455-150D | Bioingentech | 150T | EUR 1177.2 |
Parvovirus В19 One-Step PCR kit |
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| Oneq-H455-50D | Bioingentech | 50T | EUR 861.6 |
K Virus One-Step PCR kit |
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| Oneq-V365-100D | Bioingentech | 100T | EUR 1039.2 |
K Virus One-Step PCR kit |
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| Oneq-V365-150D | Bioingentech | 150T | EUR 1177.2 |
K Virus One-Step PCR kit |
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| Oneq-V365-50D | Bioingentech | 50T | EUR 861.6 |
Kudoa sp. One-Step PCR kit |
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| Oneq-V243-100D | Bioingentech | 100T | EUR 1039.2 |
Kudoa sp. One-Step PCR kit |
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| Oneq-V243-150D | Bioingentech | 150T | EUR 1177.2 |
Kudoa sp. One-Step PCR kit |
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| Oneq-V243-50D | Bioingentech | 50T | EUR 861.6 |