Comparing Reagents Used on Applied

Comparing Reagents Used on Applied Biosystem’s 3730xl

Reagents are an integral part of sequencing on Applied Biosystems (ABI) 3730xl. They allow the samples to move through the machine with ease, providing clarity and quality results. But are all reagents created equal? We compared 5x Sequencing Buffer, Dye Terminator cycle sequencing reagent, 10x Running Buffer, and Sequencing Polymer from Edge Biosystems (Edge), ABI, and McLab in order to determine how well each of them performed by evaluating array images, raw fluorescence signal, read clarity, peak uniformity, and sequencing start point.

Twelve PCR products and four plasmid samples underwent cycle sequencing using Dye Terminator cycle sequencing reagent and Sequencing Buffer from each of the three companies: Edge, ABI, and McLab. Each of the three groups of 16 samples were sequenced using Edge Running Buffer and Polymer, ABI Running Buffer and Polymer, and McLab Running Buffer and Polymer. Additionally, each group of 16 samples were also sequenced using each combination of Edge, ABI, and McLab Running Buffer with Edge, ABI, and McLab Polymer. For example, 16 samples prepared with Edge Dye Terminator and Sequencing Buffer were sequenced using: Edge Running Buffer/ABI Polymer, Edge Running Buffer/McLab Polymer, ABI Running Buffer/Edge Polymer, ABI Running Buffer/McLab Polymer, McLab Running Buffer/ABI Polymer, and McLab Running Buffer/Edge Polymer, and so on. After testing, we looked at array images for background clarity.

We compared traces for signal strength and read consistency, and we viewed the sample start point to see when each sequence began. Overall, we determined that all of the reagents were comparable, with Edge and ABI performing slightly better in signal strength, read clarity, and earlier start points.

KiloGreen 2X qPCR MasterMix-ROX
MasterMix-KR	ABM	4 x 1.25 ml - 500 reactions (20 ul)	EUR 168

TaqProbe 2X qPCR MasterMix-ROX
MasterMix-P	ABM	4 x 1.25 ml for 500 reactions (20 ul)	EUR 168

TaqProbe 2X qPCR MasterMix-iCycler
MasterMix-PC	ABM	4 x 1.25 ml for 500 reactions (20 ul)	EUR 168

TaqProbe 2X qPCR MasterMix-Multiplex
MasterMix-PM	ABM	4 x 1.25 ml for 500 reactions (20 ul)	EUR 168

BrightGreen 2X qPCR MasterMix-ROX
MasterMix-R	ABM	4 x 1.25 ml for 500 reactions (20 ul)	EUR 168

BrightGreen Express 2X qPCR MasterMix-iCycler
MasterMix-EC	ABM	4 x 1.25 ml for 500 reactions (20 ul)	EUR 168

BrightGreen Express 2X qPCR MasterMix-ROX
MasterMix-ER	ABM	4 x 1.25 ml for 500 reactions (20 ul)	EUR 168

KiloGreen 2X qPCR MasterMix-Low ROX
MasterMix-KL	ABM	4 x 1.25 ml - 500 reactions (20 ul)	EUR 168

KiloGreen 2X qPCR MasterMix-No Dye
MasterMix-KS	ABM	4 x 1.25 ml - 500 reactions (20 ul)	EUR 168

BrightGreen 2X qPCR MasterMix-Low ROX
MasterMix-LR	ABM	4 x 1.25 ml for 500 reactions (20 ul)	EUR 168

TaqProbe 2X qPCR MasterMix-Low ROX
MasterMix-PL	ABM	4 x 1.25 ml for 500 reactions (20 ul)	EUR 168

TaqProbe 2X qPCR MasterMix-No Dye
MasterMix-PS	ABM	4 x 1.25 ml for 500 reactions (20 ul)	EUR 168

BrightGreen 2X qPCR MasterMix-No Dye
MasterMix-S	ABM	4 x 1.25 ml for 500 reactions (20 ul)	EUR 168

2X PCR Taq MasterMix
G013	ABM	5.0 ml (200 Rxns)	EUR 104.4

2X PCR TaqFast MasterMix
G280	ABM	5.0 ml (200 Rxns)	EUR 160.8

Bloodirect 2X PCR MasterMix
G465	ABM	5.0 ml (200 Rxns)	EUR 166.8

Kodaq 2X PCR MasterMix
G497	ABM	5.0 ml (200 Rxns)	EUR 189.6

2X PCR Taq MasterMix
G900	ABM	25.0 ml (1000 Rxns)	EUR 246

2X PCR HotStart MasterMix
G906	ABM	5.0 ml (200 Rxns)	EUR 189.6

BrightGreen 2X qPCR MasterMix-iCycler
MasterMix-iC-XL	ABM	16 x 1.25 ml for 2000 reactions (20 ul)	EUR 451.2

BrightGreen 2X qPCR MasterMix-ROX
MasterMix-R-XL	ABM	16 x 1.25 ml for 2000 reactions (20 ul)	EUR 451.2

BrightGreen Express 2X qPCR MasterMix-Low ROX
MasterMix-EL	ABM	4 x 1.25 ml for 500 reactions (20 ul)	EUR 168

BrightGreen Express 2X qPCR MasterMix-No Dye
MasterMix-ES	ABM	4 x 1.25 ml for 500 reactions (20 ul)	EUR 168

BrightGreen 2X qPCR MasterMix-Low ROX
MasterMix-LR-XL	ABM	16 x 1.25 ml for 2000 reactions (20 ul)	EUR 451.2

BrightGreen 2X qPCR MasterMix-No Dye
MasterMix-S-XL	ABM	16 x 1.25 ml for 2000 reactions (20 ul)	EUR 451.2

2X PCR Taq Plus MasterMix
G014	ABM	5.0 ml (200 Rxns)	EUR 127.2

2X PCR Bestaq™ MasterMix
G464	ABM	5.0 ml (200 Rxns)	EUR 189.6

2X PCR Taq Plus MasterMix
G901	ABM	25.0 ml (1000 Rxns)	EUR 358.8

2X PCR SensTaq HotStart MasterMix
G939	ABM	5.0 ml (200 Rxns)	EUR 189.6

2X PCR Taq MasterMix with dye
G013-dye	ABM	5.0 ml (200 Rxns)	EUR 104.4

2X PCR TaqFast MasterMix with dye
G280-dye	ABM	5.0 ml (200 Rxns)	EUR 160.8

Kodaq 2X PCR MasterMix with dye
G497-dye	ABM	5.0 ml (200 Rxns)	EUR 189.6

2X PCR Taq MasterMix with dye
G900-dye	ABM	25.0 ml (1000 Rxns)	EUR 246

2X PCR HotStart MasterMix with dye
G906-dye	ABM	5.0 ml (200 Rxns)	EUR 189.6

2X PCR Taq Plus MasterMix with dye
G014-dye	ABM	5.0 ml (200 Rxns)	EUR 127.2

2X PCR Bestaq™ MasterMix with dye
G464-dye	ABM	5.0 ml (200 Rxns)	EUR 189.6

Safe-Green™ 2X PCR Taq MasterMix
G472	ABM	5.0 ml (200 Rxns)	EUR 127.2

2X PCR Taq Plus MasterMix with dye
G901-dye	ABM	25.0 ml (1000 Rxns)	EUR 358.8

2X PCR SensTaq HotStart MasterMix with Dye
G939-Dye	ABM	5.0 ml (200 Rxns)	EUR 189.6

Safe-Green™ 2X PCR Bestaq™ MasterMix
G478	ABM	5.0 ml (200 Rxns)	EUR 212.4

BrightGreen 5X qPCR MasterMix-iCycler
MasterMix-5C	ABM	4 x 1.0 ml for 1000 reactions (20 ul)	EUR 255.6

TaqProbe 5X qPCR MasterMix-ROX
MasterMix-5P	ABM	4 x 1.0 ml for 1000 reactions (20 ul)	EUR 255.6

TaqProbe 5X qPCR MasterMix-iCycler
MasterMix-5PC	ABM	4 x 1.0 ml for 1000 reactions (20 ul)	EUR 255.6

TaqProbe 5X qPCR MasterMix-Multiplex
MasterMix-5PM	ABM	4 x 1.0 ml for 1000 reactions (20 ul)	EUR 255.6

BrightGreen 5X qPCR MasterMix-ROX
MasterMix-5R	ABM	4 x 1.0 ml for 1000 reactions (20 ul)	EUR 255.6

BrightGreen miRNA qPCR MasterMix-iCycler
MasterMix-mC	ABM	4 x 1.25 ml for 500 reactions	EUR 181.2

BrightGreen miRNA qPCR MasterMix-ROX
MasterMix-mR	ABM	4 x 1.25 ml for 500 reactions	EUR 181.2

ACTaq? Mastermix 2x, 200 reactions
E2110-200RXN	ACTGene	each	EUR 204

ACTaq? Mastermix 2x, 50 reactions
E2110-50RXN	ACTGene	each	EUR 104.4

BrightGreen 5X qPCR MasterMix-Low ROX
MasterMix-5L	ABM	4 x 1.0 ml for 1000 reactions (20 ul)	EUR 255.6

TaqProbe 5X qPCR MasterMix-Low ROX
MasterMix-5PL	ABM	4 x 1.0 ml for 1000 reactions (20 ul)	EUR 255.6

TaqProbe 5X qPCR MasterMix-No Dye
MasterMix-5PS	ABM	4 x 1.0 ml for 1000 reactions (20 ul)	EUR 255.6

BrightGreen 5X qPCR MasterMix-No Dye
MasterMix-5S	ABM	4 x 1.0 ml for 1000 reactions (20 ul)	EUR 255.6

BrightGreen miRNA qPCR MasterMix-Low ROX
MasterMix-mL	ABM	4 x 1.25 ml for 500 reactions	EUR 181.2

BrightGreen miRNA qPCR MasterMix-No Dye
MasterMix-mS	ABM	4 x 1.25 ml for 500 reactions	EUR 181.2

Bio-Star Mastermix (2X) Low-ROX
P150	GeneOn	100 rcs (2,5 ml)	EUR 122.4

ACTaq? High Fidelity Mastermix 2x,200rxns
E2010-200RXN	ACTGene	each	EUR 270

ACTaq? High Fidelity Mastermix 2x,50rxns
E2010-50RXN	ACTGene	each	EUR 126

ACTaq? Blue Mastermix 2x, 200 rxns
E2110B-200RXN	ACTGene	each	EUR 216

ACTaq? Blue Mastermix 2x, 50 rxns
E2110B-50RXN	ACTGene	each	EUR 108

2X All-In-One High Specificity PCR MasterMix Solution, 50 µLx96 RXNS
ABP-PP-MM02961	Allele Biotech	96 RXNS	Ask for price

2X All-In-One High Specificity PCR MasterMix Solution, 10 µLx96 RXNS
ABP-PP-MM02965	Allele Biotech	96 RXNS	Ask for price

Bio-Star Mastermix with SYBR (2X) BLUE
Y220	GeneOn	100 rcs (2,5 ml)	EUR 112.8

ACTaq? Hot-Start Mastermix 2x, 200 rxns
E3510-200RXN	ACTGene	each	EUR 286.8

ACTaq? Hot-Start Mastermix 2x, 50 rxns
E3510-50RXN	ACTGene	each	EUR 132

ACTaq? Blue Hot-Strt Mastermix 2x, 200rxns
E3510B-200RXN	ACTGene	each	EUR 310.8

Bio-Star Mastermix with SYBR (2X) no dye
Y210	GeneOn	100 rcs (2,5 ml)	EUR 106.8

Bio-Star Mastermix with SYBR (2X) High-ROX
Y240	GeneOn	100 rcs (2,5 ml)	EUR 122.4

Bio-Star Mastermix with SYBR (2X) Low-ROX
Y250	GeneOn	100 rcs (2,5 ml)	EUR 122.4

ACTaq? Blue Hot-Strt Mastermix 2x, 50 rxns
E3510B-50RXN	ACTGene	each	EUR 138

Precision™ DNA Polymerase (PCR)
G078	ABM	500 U (100 ul)	EUR 133.2

LYO- RT-PCR Mastermix (one-Step)
105-600	GeneOn	192 tests (20µl/test)	EUR 627.6

LYO- RT-PCR Mastermix (one-Step)
105-600plate	GeneOn	request your format, custumized	EUR 627.6

QPCR Kit QRT-PCR Mastermix RNA
MOL6356	Scientific Laboratory Supplies	EACH	EUR 257.64

2X Multiplex PCR Premix (100Rx)
9K-002-0037	Bio Basic	100Rx, 100prep	EUR 697.37

MyTaq Blood PCR Mix, 2x
BIO-25053/S	Bioline	Sample	Ask for price

MyTaq Blood PCR Mix, 2x
BIO-25054	Bioline	250 Reactions	Ask for price

Taq 2x PCR Mix 100 rxn
BA01501	Bioatlas	100rxn	EUR 112.8
Description: High quality Taq polymerase for different PCR variations and downstream applications.

HotTaq 2x PCR Mix 100 rxn
BA01503	Bioatlas	100rxn	EUR 144
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

RedTaq 2x PCR Mix 100 rxn
BA01507	Bioatlas	100rxn	EUR 175.2
Description: High quality RedTaq polymerase for different PCR variations and downstream applications.

amfiSure ONE PCR Master Mix(2X)
P7000-005	GenDepot	5x1 ml	EUR 262.8

amfiSure ONE PCR Master Mix(2X)
P7000-010	GenDepot	10x1 ml	EUR 380.4

amfiSure ONE PCR Master Mix(2X)
P7000-050	GenDepot	50x1 ml	EUR 1453.2

amfiSure ONE PCR Master Mix(2X)
P7000-100	GenDepot	100x1 ml	EUR 2522.4

Plant Ex-Amp PCR Kit (with Kodaq MasterMix)
G923	ABM	25 preps 200 x 50ul reactions	EUR 217.2

Tissue Ex-Amp PCR Kit (with Kodaq MasterMix)
G927	ABM	100 preps 120 x 50ul reactions	EUR 156

2X Taq PCR Premix (5 x 1mL)
9K-002-0005	Bio Basic	5X1mL, 5ml	EUR 440.54

2X PCR Optimization Kit for High-Taq
9K-002-0010	Bio Basic	1mg	EUR 406.09

2X Pfu PCR Premix (5 x 1mL)
9K-002-0022	Bio Basic	5X1mL, 5ml	EUR 697.37

Smart 2X PCR pre-mix Pfu (30ul)
9K-002-0038	Bio Basic	30uL, 30uL	EUR 697.37

HotStart PCR Master mix (2X, Green Dye)
BS9291	Bio Basic	5X1mL, 5ml	EUR 251.4

HotStart PCR Master mix (2X, Green Dye)
BS9292	Bio Basic	1ml	EUR 115.68

Taq PCR Master Mix (2X, Blue Dye)
BS9295	Bio Basic	1ml	EUR 101.76

Taq PCR Master Mix (2X, Blue Dye)
BS9296	Bio Basic	5ml	EUR 227.04

Taq PCR Master Mix (2X, Red Dye)
BS9297	Bio Basic	1ml	EUR 101.76

Taq PCR Master Mix (2X, Red Dye)
BS9298	Bio Basic	5ml	EUR 227.04

amfiFusion High Fidelity PCR Master Mix(2X)
P0342-010	GenDepot	2x50 rxns	EUR 303.6

amfiFusion High Fidelity PCR Master Mix(2X)
P0342-025	GenDepot	5X50 rxns	EUR 546

amfiFusion High Fidelity PCR Master Mix(2X)
P0342-050	GenDepot	10X50 rxns	EUR 934.8

amfiFusion High Fidelity PCR Master Mix(2X)
P0342-100	GenDepot	20x50 rxns	EUR 1599.6

amfiSure Ultra Fidelity PCR Master Mix(2X)
P0346-001	GenDepot	1ml	EUR 336

amfiSure Ultra Fidelity PCR Master Mix(2X)
P0346-002	GenDepot	2x1ml	EUR 578.4

amfiSure Ultra Fidelity PCR Master Mix(2X)
P0346-005	GenDepot	5x1ml	EUR 993.6

amfiSure Ultra Fidelity PCR Master Mix(2X)
P0346-010	GenDepot	10x1ml	EUR 1632

PCR Tubes (50)
PCR-50	BioAssay Systems	50	EUR 15
Description: To be used with the PractiChrom kits in conjuction with the PiCO Explorer. Quantity: 50 PCR tubes.

Real Time PCR mastermix 2x, (No ROX), 100RXNs, optimized ready-to-use mastermix containing unique reaction buffer, dNTP, Hot-Start Taq DNA polymerase, and QPCR dye
ACT-RT400RF	ACTGene	each	EUR 261.6

Green-2-Go 1-Step qRT-PCR Mastermix-iCycler
QPCR011-iC	Bio Basic	100Rxn	EUR 287.08

MULTIPLEX KIT PCR MASTITIS PCR kit
PCR-MPX218-48D	Bioingentech	50T	EUR 543.6

MULTIPLEX KIT PCR MASTITIS PCR kit
PCR-MPX218-96D	Bioingentech	100T	EUR 686.4

Real-Time PCR Mastermix 2x, Regular Level of Rox, 100RXNs, optimized ready-to-use mastermix containing unique reaction buffer, dNTP, Hot-Start Taq DNA polymerase, and QPCR dye
ACT-RT400	ACTGene	each	EUR 261.6

Real-Time PCR Mastermix 2x, Low Level of Rox , 100RXNs, optimized ready-to-use mastermix containing unique reaction buffer, dNTP, Hot-Start Taq DNA polymerase, and QPCR dye
ACT-RT400LR	ACTGene	each	EUR 261.6

2X High-Taq PCR Premix (5 x 1mL)
9K-002-0011	Bio Basic	5X1mL, 5ml	EUR 462.47

amfiSure qGreen Q-PCR Master Mix(2X), Fluorescein
Q5601-001	GenDepot	1ml	EUR 175.2

amfiSure qGreen Q-PCR Master Mix(2X), Fluorescein
Q5601-002	GenDepot	2x1ml	EUR 248.4

amfiSure qGreen Q-PCR Master Mix(2X), Fluorescein
Q5601-005	GenDepot	5x1ml	EUR 319.2

Disaster preparedness to maintain sample and reagent integrity

• Freezers and LN2 tanks at biomolecular facilities often store irreplaceable samples and expensive reagents that must be maintained at the correct storage conditions. Temperature excursions from the recommended storage conditions may cause sample degradation and reduced reagent activity, making them unusable. Implementing a disaster preparedness plan including a robust monitoring system reduces the risk of losing samples and reagents due to natural disaster, HVAC malfunction, freezer/dewar failure and power loss.
• Cold storage devices should be located in secure locations which are unlikely to be affected by naturally occurring events including floods or hurricanes. Freezers are designed to operate in a specific temperature range and monitoring the room temperature will reduce the likelihood of devices operating outside of the recommended temperature range due to HVAC failures.
• Optimally, freezers and the monitoring system will be connected to a reliable emergency power system. Battery backup systems are available for cold storage devices and the monitoring system. Power loss can be mitigated with dry ice or a CO2/LN2 backup system. Access to the appropriate number of backup freezers or LN2 dewars ensures uninterrupted storage at the correct temperature when the primary storage device is inoperable.
• Continuous monitoring of temperature, power and the network functionality is critical to ensure the cold storage devices are operating correctly and that active alarms receive the appropriate attention. An institutional network can be augmented with additional communication devices providing an alternate path for reporting and alert notifications. Losing irreplaceable samples and reagents can be devastating. Advanced planning for natural disasters, equipment failures and power outages enables a fast, streamlined response and ensures the integrity of samples and reagents.

Long-term dry storage of enzyme-based reagents for isothermal nucleic acid amplification in a porous matrix for use in point-of-care diagnostic devices

Nucleic acid amplification test (NAAT)-based point-of-care (POC) devices are rapidly growing for use in low-resource settings. However, key challenges are the ability to store the enzyme-based reagents in dry form in the device and the long-term stability of those reagents at elevated temperatures, especially where ambient temperatures could be as high as 45 °C. Here, we describe a set of excipients including a combination of trehalose, polyethylene glycol and dextran, and a method for using them that allows long-term dry storage of enzyme-based reagents for an isothermal strand displacement amplification (iSDA) reaction in a porous matrix.

Various porous materials, including nitrocellulose, cellulose, and glass fiber, were tested. Co-dried reagents for iSDA always included those that amplified the ldh1 gene in Staphylococcus aureus (a polymerase and a nicking enzyme, 4 primers, dNTPs and a buffer). Reagents also either included a capture probe and a streptavidin-Au label required for lateral flow (LF) detection after amplification, or a fluorescent probe used for real-time detection.

The reagents showed the best stability in a glass fiber matrix when stored in the presence of 10% trehalose and 2.5% dextran. The reagents were stable for over a year at ∼22 °C as determined by lateral flow detection and gel electrophoresis. The reagents also exhibited excellent stability after 360 h at 45 °C; the assay still detected as few as 10 copies of ldh1 gene target by lateral flow detection, and 50 copies with real-time fluorescence detection. These results demonstrate the potential for incorporation of amplification reagents in dry form in point-of-care devices for use in a wide range of settings.

One-Step RT-PCR Kit with dye
G174-dye	ABM	100 x 50 ul reactions	EUR 279.6

HiScipt II One Step RT-PCR Kit
P611-01	Vazyme	50 rxn (50 μl/rxn)	EUR 234

LYO- RT-PCR Mastermix (one-Step)
105-600	GeneOn	192 tests (20µl/test)	EUR 627.6

LYO- RT-PCR Mastermix (one-Step)
105-600plate	GeneOn	request your format, custumized	EUR 627.6

igScript One Step RT-PCR Kit - 100 Reactions
4211	Intact Genomics	1/EA	EUR 284.4

igScript One Step RT-PCR Kit - 500 Reactions
4213	Intact Genomics	1/EA	EUR 908.4

Accuris One-Step RT-PCR kit, 100 reactions
PR1100-100	Benchmark Scientific	1 each	EUR 509.6

Accuris One-Step RT-PCR kit, 50 reactions
PR1100-50	Benchmark Scientific	1 each	EUR 282.5

OneScriptPlus Two-Step RT-PCR Kit
G283	ABM	25 reactions	EUR 133.2

OneScriptPlus Two-Step RT-PCR Kit
G284	ABM	100 reactions	EUR 252

amfiRivert 1-Step RT-PCR Kit
R5003-050	GenDepot	50 rxns	EUR 262.8

amfiRivert 1-Step RT-PCR Kit
R5003-100	GenDepot	100 rxns	EUR 400.8

amfiRivert 1-Step RT-PCR Kit
R5003-200	GenDepot	200 rxns	EUR 562.8

One Step RT PCR Kit (cDNA Synthesis + PCR In One Reaction)
MB1003-100Reactions	BosterBio	100 Reactions	EUR 895.2

HiScipt II One Step RT-PCR Kit (Dye Plus)
P612-01	Vazyme	50 rxn (50 μl/rxn)	EUR 234

Accuris One-Step RT-PCR kit, sample 10 reactions
PR1100-S	Benchmark Scientific	1 each	EUR 60

One-Step RT-PCR SuperMix (+dye) (4 kb)
abx098027-200rxns20ulSystems	Abbexa	200 rxns × 20 ul Systems	EUR 727.2

One-Step RT-PCR SuperMix (+dye) (8 kb)
abx098028-200rxns20ulSystems	Abbexa	200 rxns × 20 ul Systems	EUR 794.4

One-Step RT-PCR SuperMix (+dye) (8 kb)
abx098029-200rxns20ulSystems	Abbexa	200 rxns × 20 ul Systems	EUR 1028.4

amfiRivert 1-Step RT-PCR Premix
R5004-048	GenDepot	48 rxns	EUR 283.2

amfiRivert 1-Step RT-PCR Premix
R5004-096	GenDepot	96 rxns	EUR 433.2

amfiRivert 1-Step RT-PCR Premix
R5004-480	GenDepot	5x96 rxns	EUR 1113.6

Easy? One Step RT Kit
M1162-100	Biovision	each	EUR 561.6

Pospiviroid One-Step PCR kit
Oneq-A546-100R	Bioingentech	100T	EUR 1932

Pospiviroid One-Step PCR kit
Oneq-A546-150R	Bioingentech	150T	EUR 2646

Pospiviroid One-Step PCR kit
Oneq-A546-50R	Bioingentech	50T	EUR 1410

Lactobacillus One-Step PCR kit
Oneq-A566-100D	Bioingentech	100T	EUR 1039.2

Lactobacillus One-Step PCR kit
Oneq-A566-150D	Bioingentech	150T	EUR 1177.2

Lactobacillus One-Step PCR kit
Oneq-A566-50D	Bioingentech	50T	EUR 861.6

Lactococcus One-Step PCR kit
Oneq-A567-100D	Bioingentech	100T	EUR 1039.2

Lactococcus One-Step PCR kit
Oneq-A567-150D	Bioingentech	150T	EUR 1177.2

Lactococcus One-Step PCR kit
Oneq-A567-50D	Bioingentech	50T	EUR 861.6

Leuconostoc One-Step PCR kit
Oneq-A569-100D	Bioingentech	100T	EUR 1039.2

Leuconostoc One-Step PCR kit
Oneq-A569-150D	Bioingentech	150T	EUR 1177.2

Leuconostoc One-Step PCR kit
Oneq-A569-50D	Bioingentech	50T	EUR 861.6

Pediococcus One-Step PCR kit
Oneq-A571-100D	Bioingentech	100T	EUR 1039.2

Pediococcus One-Step PCR kit
Oneq-A571-150D	Bioingentech	150T	EUR 1177.2

Pediococcus One-Step PCR kit
Oneq-A571-50D	Bioingentech	50T	EUR 861.6

Halobacillus One-Step PCR kit
Oneq-A635-100D	Bioingentech	100T	EUR 1039.2

Halobacillus One-Step PCR kit
Oneq-A635-150D	Bioingentech	150T	EUR 1177.2

Halobacillus One-Step PCR kit
Oneq-A635-50D	Bioingentech	50T	EUR 861.6

Enterovirus One-Step PCR kit
Oneq-H433-100R	Bioingentech	100T	EUR 1932

Enterovirus One-Step PCR kit
Oneq-H433-150R	Bioingentech	150T	EUR 2646

Enterovirus One-Step PCR kit
Oneq-H433-50R	Bioingentech	50T	EUR 1410

Mengovirus One-Step PCR kit
Oneq-H453-100R	Bioingentech	100T	EUR 1932

Mengovirus One-Step PCR kit
Oneq-H453-150R	Bioingentech	150T	EUR 2646

Mengovirus One-Step PCR kit
Oneq-H453-50R	Bioingentech	50T	EUR 1410

Astrovirus One-Step PCR kit
Oneq-H491-100R	Bioingentech	100T	EUR 1932

Astrovirus One-Step PCR kit
Oneq-H491-150R	Bioingentech	150T	EUR 2646

Astrovirus One-Step PCR kit
Oneq-H491-50R	Bioingentech	50T	EUR 1410

Calicivirus One-Step PCR kit
Oneq-H493-100R	Bioingentech	100T	EUR 1932

Calicivirus One-Step PCR kit
Oneq-H493-150R	Bioingentech	150T	EUR 2646

Calicivirus One-Step PCR kit
Oneq-H493-50R	Bioingentech	50T	EUR 1410

Adenovirus One-Step PCR kit
Oneq-H501-100D	Bioingentech	100T	EUR 1039.2

Adenovirus One-Step PCR kit
Oneq-H501-150D	Bioingentech	150T	EUR 1177.2

Adenovirus One-Step PCR kit
Oneq-H501-50D	Bioingentech	50T	EUR 861.6

Polyomavirus One-Step PCR kit
Oneq-H515-100D	Bioingentech	100T	EUR 1039.2

Polyomavirus One-Step PCR kit
Oneq-H515-150D	Bioingentech	150T	EUR 1177.2

Polyomavirus One-Step PCR kit
Oneq-H515-50D	Bioingentech	50T	EUR 861.6

Ruminococcus One-Step PCR kit
Oneq-H619-100D	Bioingentech	100T	EUR 1039.2

Ruminococcus One-Step PCR kit
Oneq-H619-150D	Bioingentech	150T	EUR 1177.2

Ruminococcus One-Step PCR kit
Oneq-H619-50D	Bioingentech	50T	EUR 861.6

Bifidobacterium One-Step PCR kit
Oneq-H621-100D	Bioingentech	100T	EUR 1039.2

Bifidobacterium One-Step PCR kit
Oneq-H621-150D	Bioingentech	150T	EUR 1177.2

Bifidobacterium One-Step PCR kit
Oneq-H621-50D	Bioingentech	50T	EUR 861.6

Sapovirus One-Step PCR kit
Oneq-H630-100R	Bioingentech	100T	EUR 1932

Sapovirus One-Step PCR kit
Oneq-H630-150R	Bioingentech	150T	EUR 2646

Sapovirus One-Step PCR kit
Oneq-H630-50R	Bioingentech	50T	EUR 1410

Parechovirus One-Step PCR kit
Oneq-H642-100R	Bioingentech	100T	EUR 1932

Parechovirus One-Step PCR kit
Oneq-H642-150R	Bioingentech	150T	EUR 2646

Parechovirus One-Step PCR kit
Oneq-H642-50R	Bioingentech	50T	EUR 1410

Metapneumovirus One-Step PCR kit
Oneq-H643-100R	Bioingentech	100T	EUR 1932

Metapneumovirus One-Step PCR kit
Oneq-H643-150R	Bioingentech	150T	EUR 2646

Metapneumovirus One-Step PCR kit
Oneq-H643-50R	Bioingentech	50T	EUR 1410

Entamoeba One-Step PCR kit
Oneq-H683-100D	Bioingentech	100T	EUR 1039.2

Entamoeba One-Step PCR kit
Oneq-H683-150D	Bioingentech	150T	EUR 1177.2

Entamoeba One-Step PCR kit
Oneq-H683-50D	Bioingentech	50T	EUR 861.6

Acanthamoeba One-Step PCR kit
Oneq-H684-100D	Bioingentech	100T	EUR 1039.2

Acanthamoeba One-Step PCR kit
Oneq-H684-150D	Bioingentech	150T	EUR 1177.2

Acanthamoeba One-Step PCR kit
Oneq-H684-50D	Bioingentech	50T	EUR 861.6

Echovirus One-Step PCR kit
Oneq-H722-100D	Bioingentech	100T	EUR 1039.2

Echovirus One-Step PCR kit
Oneq-H722-150D	Bioingentech	150T	EUR 1177.2

Echovirus One-Step PCR kit
Oneq-H722-50D	Bioingentech	50T	EUR 861.6

ranavirus One-Step PCR kit
Oneq-V337-100D	Bioingentech	100T	EUR 1039.2

ranavirus One-Step PCR kit
Oneq-V337-150D	Bioingentech	150T	EUR 1177.2

ranavirus One-Step PCR kit
Oneq-V337-50D	Bioingentech	50T	EUR 861.6

Ruminococcus One-Step PCR kit
Oneq-V619-100D	Bioingentech	100T	EUR 896.88
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Ruminococcus.

Ruminococcus One-Step PCR kit
Oneq-V619-150D	Bioingentech	150T	EUR 1006.5
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Ruminococcus.

Ruminococcus One-Step PCR kit
Oneq-V619-50D	Bioingentech	50T	EUR 754.2
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Ruminococcus.

Bifidobacterium One-Step PCR kit
Oneq-V621-100D	Bioingentech	100T	EUR 896.88
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Bifidobacterium.

Bifidobacterium One-Step PCR kit
Oneq-V621-150D	Bioingentech	150T	EUR 1006.5
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Bifidobacterium.

Bifidobacterium One-Step PCR kit
Oneq-V621-50D	Bioingentech	50T	EUR 754.2
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Bifidobacterium.

Two-Step RT-PCR SuperMix (12 kb)
abx098025-50rxns20ulRTSystems80rxns50ulPCRSystems	Abbexa	50 rxns × 20 ul (RT Systems) / 80 rxns × 50 ul (PCR Systems)	EUR 727.2

Two-Step RT-PCR SuperMix (15 kb)
abx098026-50rxns20ulRTSystems80rxns50ulPCRSystems	Abbexa	50 rxns × 20 ul (RT Systems) / 80 rxns × 50 ul (PCR Systems)	EUR 910.8

Easy? One Step RT Kit-Dye
M1163-100	Biovision	each	EUR 561.6

PCR Mycoplasma Detection Kit
M034-Kit	TOKU-E	Kit	EUR 319.2

Wallemia sebi One-Step PCR kit
Oneq-A076-100D	Bioingentech	100T	EUR 1039.2

Wallemia sebi One-Step PCR kit
Oneq-A076-150D	Bioingentech	150T	EUR 1177.2

Wallemia sebi One-Step PCR kit
Oneq-A076-50D	Bioingentech	50T	EUR 861.6

Tenacibaculum maritimum One-Step PCR kit
Oneq-A133-100D	Bioingentech	100T	EUR 1039.2

Tenacibaculum maritimum One-Step PCR kit
Oneq-A133-150D	Bioingentech	150T	EUR 1177.2

Tenacibaculum maritimum One-Step PCR kit
Oneq-A133-50D	Bioingentech	50T	EUR 861.6

Geotrichum candidum One-Step PCR kit
Oneq-A341-100D	Bioingentech	100T	EUR 1039.2

Geotrichum candidum One-Step PCR kit
Oneq-A341-150D	Bioingentech	150T	EUR 1177.2

Geotrichum candidum One-Step PCR kit
Oneq-A341-50D	Bioingentech	50T	EUR 861.6

Beggiatoa alba One-Step PCR kit
Oneq-A350-100D	Bioingentech	100T	EUR 1039.2

Beggiatoa alba One-Step PCR kit
Oneq-A350-150D	Bioingentech	150T	EUR 1177.2

Beggiatoa alba One-Step PCR kit
Oneq-A350-50D	Bioingentech	50T	EUR 861.6

Flexibacter sp. One-Step PCR kit
Oneq-A485-100D	Bioingentech	100T	EUR 1039.2

Flexibacter sp. One-Step PCR kit
Oneq-A485-150D	Bioingentech	150T	EUR 1177.2

Flexibacter sp. One-Step PCR kit
Oneq-A485-50D	Bioingentech	50T	EUR 861.6

Thiothrix sp. One-Step PCR kit
Oneq-A526-100D	Bioingentech	100T	EUR 1039.2

Thiothrix sp. One-Step PCR kit
Oneq-A526-150D	Bioingentech	150T	EUR 1177.2

Thiothrix sp. One-Step PCR kit
Oneq-A526-50D	Bioingentech	50T	EUR 861.6

Thysanophora penicilioides One-Step PCR kit
Oneq-A527-100D	Bioingentech	100T	EUR 1039.2

Thysanophora penicilioides One-Step PCR kit
Oneq-A527-150D	Bioingentech	150T	EUR 1177.2

Thysanophora penicilioides One-Step PCR kit
Oneq-A527-50D	Bioingentech	50T	EUR 861.6

Streptococcus dysgalactiae One-Step PCR kit
Oneq-A529-100D	Bioingentech	100T	EUR 1039.2

Streptococcus dysgalactiae One-Step PCR kit
Oneq-A529-150D	Bioingentech	150T	EUR 1177.2

Streptococcus dysgalactiae One-Step PCR kit
Oneq-A529-50D	Bioingentech	50T	EUR 861.6

Aspergillus niger One-Step PCR kit
Oneq-A530-100D	Bioingentech	100T	EUR 1039.2

Aspergillus niger One-Step PCR kit
Oneq-A530-150D	Bioingentech	150T	EUR 1177.2

Aspergillus niger One-Step PCR kit
Oneq-A530-50D	Bioingentech	50T	EUR 861.6

Botrytis cinerea One-Step PCR kit
Oneq-A531-100D	Bioingentech	100T	EUR 1039.2

Botrytis cinerea One-Step PCR kit
Oneq-A531-150D	Bioingentech	150T	EUR 1177.2

Botrytis cinerea One-Step PCR kit
Oneq-A531-50D	Bioingentech	50T	EUR 861.6

Cladosporium cladosporioides One-Step PCR kit
Oneq-A532-100D	Bioingentech	100T	EUR 1474.8

Cladosporium cladosporioides One-Step PCR kit
Oneq-A532-150D	Bioingentech	150T	EUR 2005.2

Cladosporium cladosporioides One-Step PCR kit
Oneq-A532-50D	Bioingentech	50T	EUR 1149.6

Talaromyces flavus One-Step PCR kit
Oneq-A533-100D	Bioingentech	100T	EUR 1039.2

Talaromyces flavus One-Step PCR kit
Oneq-A533-150D	Bioingentech	150T	EUR 1177.2

Talaromyces flavus One-Step PCR kit
Oneq-A533-50D	Bioingentech	50T	EUR 861.6

Erwinia amylovora One-Step PCR kit
Oneq-A534-100D	Bioingentech	100T	EUR 1039.2

Erwinia amylovora One-Step PCR kit
Oneq-A534-150D	Bioingentech	150T	EUR 1177.2

Erwinia amylovora One-Step PCR kit
Oneq-A534-50D	Bioingentech	50T	EUR 861.6

Penicillium spp. One-Step PCR kit
Oneq-A535-100D	Bioingentech	100T	EUR 1039.2

Penicillium spp. One-Step PCR kit
Oneq-A535-150D	Bioingentech	150T	EUR 1177.2

Penicillium spp. One-Step PCR kit
Oneq-A535-50D	Bioingentech	50T	EUR 861.6

Phomopsis viticola One-Step PCR kit
Oneq-A536-100D	Bioingentech	100T	EUR 1039.2

Phomopsis viticola One-Step PCR kit
Oneq-A536-150D	Bioingentech	150T	EUR 1177.2

Phomopsis viticola One-Step PCR kit
Oneq-A536-50D	Bioingentech	50T	EUR 861.6

Saccharomyces cerevisiae One-Step PCR kit
Oneq-A537-100D	Bioingentech	100T	EUR 1039.2

Saccharomyces cerevisiae One-Step PCR kit
Oneq-A537-150D	Bioingentech	150T	EUR 1177.2

Saccharomyces cerevisiae One-Step PCR kit
Oneq-A537-50D	Bioingentech	50T	EUR 861.6

Acetobacter sp One-Step PCR kit
Oneq-A551-100D	Bioingentech	100T	EUR 1039.2

Acetobacter sp One-Step PCR kit
Oneq-A551-150D	Bioingentech	150T	EUR 1177.2

Acetobacter sp One-Step PCR kit
Oneq-A551-50D	Bioingentech	50T	EUR 861.6

Alicyclobacillus acidiphilus One-Step PCR kit
Oneq-A552-100D	Bioingentech	100T	EUR 1039.2

Alicyclobacillus acidiphilus One-Step PCR kit
Oneq-A552-150D	Bioingentech	150T	EUR 1177.2

Alicyclobacillus acidiphilus One-Step PCR kit
Oneq-A552-50D	Bioingentech	50T	EUR 861.6

Alicyclobacillus acidocaldarius One-Step PCR kit
Oneq-A553-100D	Bioingentech	100T	EUR 1039.2

Alicyclobacillus acidocaldarius One-Step PCR kit
Oneq-A553-150D	Bioingentech	150T	EUR 1177.2

Alicyclobacillus acidocaldarius One-Step PCR kit
Oneq-A553-50D	Bioingentech	50T	EUR 861.6

Alicyclobacillus acidoterrestris One-Step PCR kit
Oneq-A554-100D	Bioingentech	100T	EUR 1039.2

Alicyclobacillus acidoterrestris One-Step PCR kit
Oneq-A554-150D	Bioingentech	150T	EUR 1177.2

Alicyclobacillus acidoterrestris One-Step PCR kit
Oneq-A554-50D	Bioingentech	50T	EUR 861.6

Alicyclobacillus herbarius One-Step PCR kit
Oneq-A555-100D	Bioingentech	100T	EUR 1039.2

Alicyclobacillus herbarius One-Step PCR kit
Oneq-A555-150D	Bioingentech	150T	EUR 1177.2

Alicyclobacillus herbarius One-Step PCR kit
Oneq-A555-50D	Bioingentech	50T	EUR 861.6

Asaia bogorensis One-Step PCR kit
Oneq-A556-100D	Bioingentech	100T	EUR 1039.2

Asaia bogorensis One-Step PCR kit
Oneq-A556-150D	Bioingentech	150T	EUR 1177.2

Asaia bogorensis One-Step PCR kit
Oneq-A556-50D	Bioingentech	50T	EUR 861.6

Asaia lannensis One-Step PCR kit
Oneq-A557-100D	Bioingentech	100T	EUR 1039.2

Asaia lannensis One-Step PCR kit
Oneq-A557-150D	Bioingentech	150T	EUR 1177.2

Asaia lannensis One-Step PCR kit
Oneq-A557-50D	Bioingentech	50T	EUR 861.6

Aspergillus flavus One-Step PCR kit
Oneq-A558-100D	Bioingentech	100T	EUR 1039.2

Aspergillus flavus One-Step PCR kit
Oneq-A558-150D	Bioingentech	150T	EUR 1177.2

Aspergillus flavus One-Step PCR kit
Oneq-A558-50D	Bioingentech	50T	EUR 861.6

Aspergillus parasticus One-Step PCR kit
Oneq-A559-100D	Bioingentech	100T	EUR 1039.2

Aspergillus parasticus One-Step PCR kit
Oneq-A559-150D	Bioingentech	150T	EUR 1177.2

Aspergillus parasticus One-Step PCR kit
Oneq-A559-50D	Bioingentech	50T	EUR 861.6

Aureobasidium pullulans One-Step PCR kit
Oneq-A560-100D	Bioingentech	100T	EUR 1039.2

Aureobasidium pullulans One-Step PCR kit
Oneq-A560-150D	Bioingentech	150T	EUR 1177.2

Aureobasidium pullulans One-Step PCR kit
Oneq-A560-50D	Bioingentech	50T	EUR 861.6

Byssochlamys nivea One-Step PCR kit
Oneq-A561-100D	Bioingentech	100T	EUR 1039.2

Byssochlamys nivea One-Step PCR kit
Oneq-A561-150D	Bioingentech	150T	EUR 1177.2

Byssochlamys nivea One-Step PCR kit
Oneq-A561-50D	Bioingentech	50T	EUR 861.6