Comparing Reagents Used on Applied

Comparing Reagents Used on Applied Biosystem’s 3730xl

Reagents are an integral part of sequencing on Applied Biosystems (ABI) 3730xl. They allow the samples to move through the machine with ease, providing clarity and quality results. But are all reagents created equal? We compared 5x Sequencing Buffer, Dye Terminator cycle sequencing reagent, 10x Running Buffer, and Sequencing Polymer from Edge Biosystems (Edge), ABI, and McLab in order to determine how well each of them performed by evaluating array images, raw fluorescence signal, read clarity, peak uniformity, and sequencing start point.

Twelve PCR products and four plasmid samples underwent cycle sequencing using Dye Terminator cycle sequencing reagent and Sequencing Buffer from each of the three companies: Edge, ABI, and McLab. Each of the three groups of 16 samples were sequenced using Edge Running Buffer and Polymer, ABI Running Buffer and Polymer, and McLab Running Buffer and Polymer. Additionally, each group of 16 samples were also sequenced using each combination of Edge, ABI, and McLab Running Buffer with Edge, ABI, and McLab Polymer. For example, 16 samples prepared with Edge Dye Terminator and Sequencing Buffer were sequenced using: Edge Running Buffer/ABI Polymer, Edge Running Buffer/McLab Polymer, ABI Running Buffer/Edge Polymer, ABI Running Buffer/McLab Polymer, McLab Running Buffer/ABI Polymer, and McLab Running Buffer/Edge Polymer, and so on. After testing, we looked at array images for background clarity.

We compared traces for signal strength and read consistency, and we viewed the sample start point to see when each sequence began. Overall, we determined that all of the reagents were comparable, with Edge and ABI performing slightly better in signal strength, read clarity, and earlier start points.

KiloGreen 2X qPCR MasterMix-ROX
MasterMix-KR	ABM	4 x 1.25 ml - 500 reactions (20 ul)	EUR 140

TaqProbe 2X qPCR MasterMix-ROX
MasterMix-P	ABM	4 x 1.25 ml for 500 reactions (20 ul)	EUR 140

TaqProbe 2X qPCR MasterMix-iCycler
MasterMix-PC	ABM	4 x 1.25 ml for 500 reactions (20 ul)	EUR 140

TaqProbe 2X qPCR MasterMix-Multiplex
MasterMix-PM	ABM	4 x 1.25 ml for 500 reactions (20 ul)	EUR 140

BrightGreen 2X qPCR MasterMix-ROX
MasterMix-R	ABM	4 x 1.25 ml for 500 reactions (20 ul)	EUR 140

2X PCR Taq MasterMix
G013	ABM	5.0 ml (200 Rxns)	EUR 87

2X PCR TaqFast MasterMix
G280	ABM	5.0 ml (200 Rxns)	EUR 134

Bloodirect 2X PCR MasterMix
G465	ABM	5.0 ml (200 Rxns)	EUR 139

Kodaq 2X PCR MasterMix
G497	ABM	5.0 ml (200 Rxns)	EUR 158

2X PCR Taq MasterMix
G900	ABM	25.0 ml (1000 Rxns)	EUR 205

2X PCR HotStart MasterMix
G906	ABM	5.0 ml (200 Rxns)	EUR 158

BrightGreen Express 2X qPCR MasterMix-iCycler
MasterMix-EC	ABM	4 x 1.25 ml for 500 reactions (20 ul)	EUR 140

BrightGreen Express 2X qPCR MasterMix-ROX
MasterMix-ER	ABM	4 x 1.25 ml for 500 reactions (20 ul)	EUR 140

KiloGreen 2X qPCR MasterMix-Low ROX
MasterMix-KL	ABM	4 x 1.25 ml - 500 reactions (20 ul)	EUR 140

KiloGreen 2X qPCR MasterMix-No Dye
MasterMix-KS	ABM	4 x 1.25 ml - 500 reactions (20 ul)	EUR 140

BrightGreen 2X qPCR MasterMix-Low ROX
MasterMix-LR	ABM	4 x 1.25 ml for 500 reactions (20 ul)	EUR 140

TaqProbe 2X qPCR MasterMix-Low ROX
MasterMix-PL	ABM	4 x 1.25 ml for 500 reactions (20 ul)	EUR 140

TaqProbe 2X qPCR MasterMix-No Dye
MasterMix-PS	ABM	4 x 1.25 ml for 500 reactions (20 ul)	EUR 140

BrightGreen 2X qPCR MasterMix-No Dye
MasterMix-S	ABM	4 x 1.25 ml for 500 reactions (20 ul)	EUR 140

BrightGreen 2X qPCR MasterMix-iCycler
MasterMix-iC-XL	ABM	16 x 1.25 ml for 2000 reactions (20 ul)	EUR 376

BrightGreen 2X qPCR MasterMix-ROX
MasterMix-R-XL	ABM	16 x 1.25 ml for 2000 reactions (20 ul)	EUR 376

BrightGreen Express 2X qPCR MasterMix-Low ROX
MasterMix-EL	ABM	4 x 1.25 ml for 500 reactions (20 ul)	EUR 140

BrightGreen Express 2X qPCR MasterMix-No Dye
MasterMix-ES	ABM	4 x 1.25 ml for 500 reactions (20 ul)	EUR 140

BrightGreen 2X qPCR MasterMix-Low ROX
MasterMix-LR-XL	ABM	16 x 1.25 ml for 2000 reactions (20 ul)	EUR 376

BrightGreen 2X qPCR MasterMix-No Dye
MasterMix-S-XL	ABM	16 x 1.25 ml for 2000 reactions (20 ul)	EUR 376

2X PCR Taq Plus MasterMix
G014	ABM	5.0 ml (200 Rxns)	EUR 106

2X PCR Bestaq™ MasterMix
G464	ABM	5.0 ml (200 Rxns)	EUR 158

2X PCR Taq Plus MasterMix
G901	ABM	25.0 ml (1000 Rxns)	EUR 299

2X PCR SensTaq HotStart MasterMix
G939	ABM	5.0 ml (200 Rxns)	EUR 158

2X PCR Taq MasterMix with dye
G013-dye	ABM	5.0 ml (200 Rxns)	EUR 87

2X PCR TaqFast MasterMix with dye
G280-dye	ABM	5.0 ml (200 Rxns)	EUR 134

Kodaq 2X PCR MasterMix with dye
G497-dye	ABM	5.0 ml (200 Rxns)	EUR 158

2X PCR Taq MasterMix with dye
G900-dye	ABM	25.0 ml (1000 Rxns)	EUR 205

2X PCR HotStart MasterMix with dye
G906-dye	ABM	5.0 ml (200 Rxns)	EUR 158

2X PCR Taq Plus MasterMix with dye
G014-dye	ABM	5.0 ml (200 Rxns)	EUR 106

2X PCR Bestaq™ MasterMix with dye
G464-dye	ABM	5.0 ml (200 Rxns)	EUR 158

Safe-Green™ 2X PCR Taq MasterMix
G472	ABM	5.0 ml (200 Rxns)	EUR 106

2X PCR Taq Plus MasterMix with dye
G901-dye	ABM	25.0 ml (1000 Rxns)	EUR 299

2X PCR SensTaq HotStart MasterMix with Dye
G939-Dye	ABM	5.0 ml (200 Rxns)	EUR 158

Safe-Green™ 2X PCR Bestaq™ MasterMix
G478	ABM	5.0 ml (200 Rxns)	EUR 177

ACTaq? Mastermix 2x, 200 reactions
E2110-200RXN	ACTGene		EUR 170

ACTaq? Mastermix 2x, 50 reactions
E2110-50RXN	ACTGene		EUR 87

BrightGreen 5X qPCR MasterMix-iCycler
MasterMix-5C	ABM	4 x 1.0 ml for 1000 reactions (20 ul)	EUR 213

TaqProbe 5X qPCR MasterMix-ROX
MasterMix-5P	ABM	4 x 1.0 ml for 1000 reactions (20 ul)	EUR 213

TaqProbe 5X qPCR MasterMix-iCycler
MasterMix-5PC	ABM	4 x 1.0 ml for 1000 reactions (20 ul)	EUR 213

TaqProbe 5X qPCR MasterMix-Multiplex
MasterMix-5PM	ABM	4 x 1.0 ml for 1000 reactions (20 ul)	EUR 213

BrightGreen 5X qPCR MasterMix-ROX
MasterMix-5R	ABM	4 x 1.0 ml for 1000 reactions (20 ul)	EUR 213

BrightGreen miRNA qPCR MasterMix-iCycler
MasterMix-mC	ABM	4 x 1.25 ml for 500 reactions	EUR 151

BrightGreen miRNA qPCR MasterMix-ROX
MasterMix-mR	ABM	4 x 1.25 ml for 500 reactions	EUR 151

BrightGreen 5X qPCR MasterMix-Low ROX
MasterMix-5L	ABM	4 x 1.0 ml for 1000 reactions (20 ul)	EUR 213

TaqProbe 5X qPCR MasterMix-Low ROX
MasterMix-5PL	ABM	4 x 1.0 ml for 1000 reactions (20 ul)	EUR 213

TaqProbe 5X qPCR MasterMix-No Dye
MasterMix-5PS	ABM	4 x 1.0 ml for 1000 reactions (20 ul)	EUR 213

BrightGreen 5X qPCR MasterMix-No Dye
MasterMix-5S	ABM	4 x 1.0 ml for 1000 reactions (20 ul)	EUR 213

BrightGreen miRNA qPCR MasterMix-Low ROX
MasterMix-mL	ABM	4 x 1.25 ml for 500 reactions	EUR 151

BrightGreen miRNA qPCR MasterMix-No Dye
MasterMix-mS	ABM	4 x 1.25 ml for 500 reactions	EUR 151

Precision™ DNA Polymerase (PCR)
G078	ABM	500 U (100 ul)	EUR 111

Bio-Star Mastermix (2X) Low-ROX
P150	GeneOn	100 rcs (2,5 ml)	EUR 102

ACTaq? High Fidelity Mastermix 2x,200rxns
E2010-200RXN	ACTGene		EUR 225

ACTaq? High Fidelity Mastermix 2x,50rxns
E2010-50RXN	ACTGene		EUR 105

ACTaq? Blue Mastermix 2x, 200 rxns
E2110B-200RXN	ACTGene		EUR 180

ACTaq? Blue Mastermix 2x, 50 rxns
E2110B-50RXN	ACTGene		EUR 90

2X All-In-One High Specificity PCR MasterMix Solution, 50 µLx96 RXNS
ABP-PP-MM02961	Allele Biotech	96 RXNS	Ask for price

2X All-In-One High Specificity PCR MasterMix Solution, 10 µLx96 RXNS
ABP-PP-MM02965	Allele Biotech	96 RXNS	Ask for price

ACTaq? Hot-Start Mastermix 2x, 200 rxns
E3510-200RXN	ACTGene		EUR 239

ACTaq? Hot-Start Mastermix 2x, 50 rxns
E3510-50RXN	ACTGene		EUR 110

ACTaq? Blue Hot-Strt Mastermix 2x, 200rxns
E3510B-200RXN	ACTGene		EUR 259

ACTaq? Blue Hot-Strt Mastermix 2x, 50 rxns
E3510B-50RXN	ACTGene		EUR 115

2X Multiplex PCR Premix (100Rx)
9K-002-0037	Bio Basic	100Rx, 100prep	EUR 581.14

MyTaq Blood PCR Mix, 2x
BIO-25053/S	Bioline	Sample	Ask for price

MyTaq Blood PCR Mix, 2x
BIO-25054	Bioline	250 Reactions	Ask for price

LYO- RT-PCR Mastermix (one-Step)
105-600	GeneOn	192 tests (20µl/test)	EUR 523

LYO- RT-PCR Mastermix (one-Step)
105-600plate	GeneOn	request your format, custumized	EUR 523

Taq 2x PCR Mix 100 rxn
BA01501	Bioatlas	100rxn	EUR 94
Description: High quality Taq polymerase for different PCR variations and downstream applications.

HotTaq 2x PCR Mix 100 rxn
BA01503	Bioatlas	100rxn	EUR 120
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

RedTaq 2x PCR Mix 100 rxn
BA01507	Bioatlas	100rxn	EUR 146
Description: High quality RedTaq polymerase for different PCR variations and downstream applications.

amfiSure ONE PCR Master Mix(2X)
P7000-005	GenDepot	5x1 ml	EUR 219

amfiSure ONE PCR Master Mix(2X)
P7000-010	GenDepot	10x1 ml	EUR 317

amfiSure ONE PCR Master Mix(2X)
P7000-050	GenDepot	50x1 ml	EUR 1211

amfiSure ONE PCR Master Mix(2X)
P7000-100	GenDepot	100x1 ml	EUR 2102

Plant Ex-Amp PCR Kit (with Kodaq MasterMix)
G923	ABM	25 preps 200 x 50ul reactions	EUR 181

Tissue Ex-Amp PCR Kit (with Kodaq MasterMix)
G927	ABM	100 preps 120 x 50ul reactions	EUR 130

HotStart PCR Master mix (2X, Green Dye)
BS9291	Bio Basic	5X1mL, 5ml	EUR 209.5

HotStart PCR Master mix (2X, Green Dye)
BS9292	Bio Basic	1ml	EUR 96.4

Taq PCR Master Mix (2X, Blue Dye)
BS9295	Bio Basic	1ml	EUR 84.8

Taq PCR Master Mix (2X, Blue Dye)
BS9296	Bio Basic	5ml	EUR 189.2

Taq PCR Master Mix (2X, Red Dye)
BS9297	Bio Basic	1ml	EUR 84.8

Taq PCR Master Mix (2X, Red Dye)
BS9298	Bio Basic	5ml	EUR 189.2

2X Taq PCR Premix (5 x 1mL)
9K-002-0005	Bio Basic	5X1mL, 5ml	EUR 367.12

2X PCR Optimization Kit for High-Taq
9K-002-0010	Bio Basic	1mg	EUR 338.41

2X Pfu PCR Premix (5 x 1mL)
9K-002-0022	Bio Basic	5X1mL, 5ml	EUR 581.14

Smart 2X PCR pre-mix Pfu (30ul)
9K-002-0038	Bio Basic	30uL, 30uL	EUR 581.14

amfiFusion High Fidelity PCR Master Mix(2X)
P0342-010	GenDepot	2x50 rxns	EUR 253

amfiFusion High Fidelity PCR Master Mix(2X)
P0342-025	GenDepot	5X50 rxns	EUR 455

amfiFusion High Fidelity PCR Master Mix(2X)
P0342-050	GenDepot	10X50 rxns	EUR 779

amfiFusion High Fidelity PCR Master Mix(2X)
P0342-100	GenDepot	20x50 rxns	EUR 1333

amfiSure Ultra Fidelity PCR Master Mix(2X)
P0346-001	GenDepot	1ml	EUR 280

Disaster preparedness to maintain sample and reagent integrity

• Freezers and LN2 tanks at biomolecular facilities often store irreplaceable samples and expensive reagents that must be maintained at the correct storage conditions. Temperature excursions from the recommended storage conditions may cause sample degradation and reduced reagent activity, making them unusable. Implementing a disaster preparedness plan including a robust monitoring system reduces the risk of losing samples and reagents due to natural disaster, HVAC malfunction, freezer/dewar failure and power loss.
• Cold storage devices should be located in secure locations which are unlikely to be affected by naturally occurring events including floods or hurricanes. Freezers are designed to operate in a specific temperature range and monitoring the room temperature will reduce the likelihood of devices operating outside of the recommended temperature range due to HVAC failures.
• Optimally, freezers and the monitoring system will be connected to a reliable emergency power system. Battery backup systems are available for cold storage devices and the monitoring system. Power loss can be mitigated with dry ice or a CO2/LN2 backup system. Access to the appropriate number of backup freezers or LN2 dewars ensures uninterrupted storage at the correct temperature when the primary storage device is inoperable.
• Continuous monitoring of temperature, power and the network functionality is critical to ensure the cold storage devices are operating correctly and that active alarms receive the appropriate attention. An institutional network can be augmented with additional communication devices providing an alternate path for reporting and alert notifications. Losing irreplaceable samples and reagents can be devastating. Advanced planning for natural disasters, equipment failures and power outages enables a fast, streamlined response and ensures the integrity of samples and reagents.

Long-term dry storage of enzyme-based reagents for isothermal nucleic acid amplification in a porous matrix for use in point-of-care diagnostic devices

Nucleic acid amplification test (NAAT)-based point-of-care (POC) devices are rapidly growing for use in low-resource settings. However, key challenges are the ability to store the enzyme-based reagents in dry form in the device and the long-term stability of those reagents at elevated temperatures, especially where ambient temperatures could be as high as 45 °C. Here, we describe a set of excipients including a combination of trehalose, polyethylene glycol and dextran, and a method for using them that allows long-term dry storage of enzyme-based reagents for an isothermal strand displacement amplification (iSDA) reaction in a porous matrix.

Various porous materials, including nitrocellulose, cellulose, and glass fiber, were tested. Co-dried reagents for iSDA always included those that amplified the ldh1 gene in Staphylococcus aureus (a polymerase and a nicking enzyme, 4 primers, dNTPs and a buffer). Reagents also either included a capture probe and a streptavidin-Au label required for lateral flow (LF) detection after amplification, or a fluorescent probe used for real-time detection.

The reagents showed the best stability in a glass fiber matrix when stored in the presence of 10% trehalose and 2.5% dextran. The reagents were stable for over a year at ∼22 °C as determined by lateral flow detection and gel electrophoresis. The reagents also exhibited excellent stability after 360 h at 45 °C; the assay still detected as few as 10 copies of ldh1 gene target by lateral flow detection, and 50 copies with real-time fluorescence detection. These results demonstrate the potential for incorporation of amplification reagents in dry form in point-of-care devices for use in a wide range of settings.

One-Step RT-PCR Kit with dye
G174-dye	ABM	100 x 50 ul reactions	EUR 233

HiScipt II One Step RT-PCR Kit
P611-01	Vazyme	50 rxn (50 μl/rxn)	EUR 195

LYO- RT-PCR Mastermix (one-Step)
105-600	GeneOn	192 tests (20µl/test)	EUR 523

LYO- RT-PCR Mastermix (one-Step)
105-600plate	GeneOn	request your format, custumized	EUR 523

amfiRivert 1-Step RT-PCR Kit
R5003-050	GenDepot	50 rxns	EUR 219

amfiRivert 1-Step RT-PCR Kit
R5003-100	GenDepot	100 rxns	EUR 334

amfiRivert 1-Step RT-PCR Kit
R5003-200	GenDepot	200 rxns	EUR 469

OneScriptPlus Two-Step RT-PCR Kit
G283	ABM	25 reactions	EUR 111

OneScriptPlus Two-Step RT-PCR Kit
G284	ABM	100 reactions	EUR 210

igScript One Step RT-PCR Kit - 100 Reactions
4211	Intact Genomics	1/EA	EUR 237

igScript One Step RT-PCR Kit - 500 Reactions
4213	Intact Genomics	1/EA	EUR 757

One Step RT PCR Kit (cDNA Synthesis + PCR In One Reaction)
MB1003-100Reactions	BosterBio	100 Reactions	EUR 746

HiScipt II One Step RT-PCR Kit (Dye Plus)
P612-01	Vazyme	50 rxn (50 μl/rxn)	EUR 195

amfiRivert 1-Step RT-PCR Premix
R5004-048	GenDepot	48 rxns	EUR 236

amfiRivert 1-Step RT-PCR Premix
R5004-096	GenDepot	96 rxns	EUR 361

amfiRivert 1-Step RT-PCR Premix
R5004-480	GenDepot	5x96 rxns	EUR 928

One-Step RT-PCR SuperMix (+dye) (4 kb)
abx098027-200rxns20ulSystems	Abbexa	200 rxns × 20 ul Systems	EUR 606

One-Step RT-PCR SuperMix (+dye) (8 kb)
abx098028-200rxns20ulSystems	Abbexa	200 rxns × 20 ul Systems	EUR 662

One-Step RT-PCR SuperMix (+dye) (8 kb)
abx098029-200rxns20ulSystems	Abbexa	200 rxns × 20 ul Systems	EUR 857

Easy? One Step RT Kit
M1162-100	Biovision		EUR 468

Two-Step RT-PCR SuperMix (12 kb)
abx098025-50rxns20ulRTSystems80rxns50ulPCRSystems	Abbexa	50 rxns × 20 ul (RT Systems) / 80 rxns × 50 ul (PCR Systems)	EUR 606

Two-Step RT-PCR SuperMix (15 kb)
abx098026-50rxns20ulRTSystems80rxns50ulPCRSystems	Abbexa	50 rxns × 20 ul (RT Systems) / 80 rxns × 50 ul (PCR Systems)	EUR 759

Pospiviroid One-Step PCR kit
Oneq-A546-100R	Bioingentech	100T	EUR 1610

Pospiviroid One-Step PCR kit
Oneq-A546-150R	Bioingentech	150T	EUR 2205

Pospiviroid One-Step PCR kit
Oneq-A546-50R	Bioingentech	50T	EUR 1175

Lactobacillus One-Step PCR kit
Oneq-A566-100D	Bioingentech	100T	EUR 866

Lactobacillus One-Step PCR kit
Oneq-A566-150D	Bioingentech	150T	EUR 981

Lactobacillus One-Step PCR kit
Oneq-A566-50D	Bioingentech	50T	EUR 718

Lactococcus One-Step PCR kit
Oneq-A567-100D	Bioingentech	100T	EUR 866

Lactococcus One-Step PCR kit
Oneq-A567-150D	Bioingentech	150T	EUR 981

Lactococcus One-Step PCR kit
Oneq-A567-50D	Bioingentech	50T	EUR 718

Leuconostoc One-Step PCR kit
Oneq-A569-100D	Bioingentech	100T	EUR 866

Leuconostoc One-Step PCR kit
Oneq-A569-150D	Bioingentech	150T	EUR 981

Leuconostoc One-Step PCR kit
Oneq-A569-50D	Bioingentech	50T	EUR 718

Pediococcus One-Step PCR kit
Oneq-A571-100D	Bioingentech	100T	EUR 866

Pediococcus One-Step PCR kit
Oneq-A571-150D	Bioingentech	150T	EUR 981

Pediococcus One-Step PCR kit
Oneq-A571-50D	Bioingentech	50T	EUR 718

Halobacillus One-Step PCR kit
Oneq-A635-100D	Bioingentech	100T	EUR 866

Halobacillus One-Step PCR kit
Oneq-A635-150D	Bioingentech	150T	EUR 981

Halobacillus One-Step PCR kit
Oneq-A635-50D	Bioingentech	50T	EUR 718

Enterovirus One-Step PCR kit
Oneq-H433-100R	Bioingentech	100T	EUR 1610

Enterovirus One-Step PCR kit
Oneq-H433-150R	Bioingentech	150T	EUR 2205

Enterovirus One-Step PCR kit
Oneq-H433-50R	Bioingentech	50T	EUR 1175

Mengovirus One-Step PCR kit
Oneq-H453-100R	Bioingentech	100T	EUR 1610

Mengovirus One-Step PCR kit
Oneq-H453-150R	Bioingentech	150T	EUR 2205

Mengovirus One-Step PCR kit
Oneq-H453-50R	Bioingentech	50T	EUR 1175

Astrovirus One-Step PCR kit
Oneq-H491-100R	Bioingentech	100T	EUR 1610

Astrovirus One-Step PCR kit
Oneq-H491-150R	Bioingentech	150T	EUR 2205

Astrovirus One-Step PCR kit
Oneq-H491-50R	Bioingentech	50T	EUR 1175

Calicivirus One-Step PCR kit
Oneq-H493-100R	Bioingentech	100T	EUR 1610

Calicivirus One-Step PCR kit
Oneq-H493-150R	Bioingentech	150T	EUR 2205

Calicivirus One-Step PCR kit
Oneq-H493-50R	Bioingentech	50T	EUR 1175

Adenovirus One-Step PCR kit
Oneq-H501-100D	Bioingentech	100T	EUR 866

Adenovirus One-Step PCR kit
Oneq-H501-150D	Bioingentech	150T	EUR 981

Adenovirus One-Step PCR kit
Oneq-H501-50D	Bioingentech	50T	EUR 718

Polyomavirus One-Step PCR kit
Oneq-H515-100D	Bioingentech	100T	EUR 866

Polyomavirus One-Step PCR kit
Oneq-H515-150D	Bioingentech	150T	EUR 981

Polyomavirus One-Step PCR kit
Oneq-H515-50D	Bioingentech	50T	EUR 718

Ruminococcus One-Step PCR kit
Oneq-H619-100D	Bioingentech	100T	EUR 866

Ruminococcus One-Step PCR kit
Oneq-H619-150D	Bioingentech	150T	EUR 981

Ruminococcus One-Step PCR kit
Oneq-H619-50D	Bioingentech	50T	EUR 718

Bifidobacterium One-Step PCR kit
Oneq-H621-100D	Bioingentech	100T	EUR 866

Bifidobacterium One-Step PCR kit
Oneq-H621-150D	Bioingentech	150T	EUR 981

Bifidobacterium One-Step PCR kit
Oneq-H621-50D	Bioingentech	50T	EUR 718

Sapovirus One-Step PCR kit
Oneq-H630-100R	Bioingentech	100T	EUR 1610

Sapovirus One-Step PCR kit
Oneq-H630-150R	Bioingentech	150T	EUR 2205

Sapovirus One-Step PCR kit
Oneq-H630-50R	Bioingentech	50T	EUR 1175

Parechovirus One-Step PCR kit
Oneq-H642-100R	Bioingentech	100T	EUR 1610

Parechovirus One-Step PCR kit
Oneq-H642-150R	Bioingentech	150T	EUR 2205

Parechovirus One-Step PCR kit
Oneq-H642-50R	Bioingentech	50T	EUR 1175

Metapneumovirus One-Step PCR kit
Oneq-H643-100R	Bioingentech	100T	EUR 1610

Metapneumovirus One-Step PCR kit
Oneq-H643-150R	Bioingentech	150T	EUR 2205

Metapneumovirus One-Step PCR kit
Oneq-H643-50R	Bioingentech	50T	EUR 1175

Entamoeba One-Step PCR kit
Oneq-H683-100D	Bioingentech	100T	EUR 866

Entamoeba One-Step PCR kit
Oneq-H683-150D	Bioingentech	150T	EUR 981

Entamoeba One-Step PCR kit
Oneq-H683-50D	Bioingentech	50T	EUR 718

Acanthamoeba One-Step PCR kit
Oneq-H684-100D	Bioingentech	100T	EUR 866

Acanthamoeba One-Step PCR kit
Oneq-H684-150D	Bioingentech	150T	EUR 981

Acanthamoeba One-Step PCR kit
Oneq-H684-50D	Bioingentech	50T	EUR 718

Echovirus One-Step PCR kit
Oneq-H722-100D	Bioingentech	100T	EUR 866

Echovirus One-Step PCR kit
Oneq-H722-150D	Bioingentech	150T	EUR 981

Echovirus One-Step PCR kit
Oneq-H722-50D	Bioingentech	50T	EUR 718

ranavirus One-Step PCR kit
Oneq-V337-100D	Bioingentech	100T	EUR 866

ranavirus One-Step PCR kit
Oneq-V337-150D	Bioingentech	150T	EUR 981

ranavirus One-Step PCR kit
Oneq-V337-50D	Bioingentech	50T	EUR 718

Ruminococcus One-Step PCR kit
Oneq-V619-100D	Bioingentech	100T	EUR 747.4
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Ruminococcus.

Ruminococcus One-Step PCR kit
Oneq-V619-150D	Bioingentech	150T	EUR 838.75
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Ruminococcus.

Ruminococcus One-Step PCR kit
Oneq-V619-50D	Bioingentech	50T	EUR 628.5
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Ruminococcus.

Bifidobacterium One-Step PCR kit
Oneq-V621-100D	Bioingentech	100T	EUR 747.4
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Bifidobacterium.

Bifidobacterium One-Step PCR kit
Oneq-V621-150D	Bioingentech	150T	EUR 838.75
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Bifidobacterium.

Bifidobacterium One-Step PCR kit
Oneq-V621-50D	Bioingentech	50T	EUR 628.5
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Bifidobacterium.

PCR Mycoplasma Detection Kit
M034-Kit	TOKU-E	Kit	EUR 266