
Isolation of Total RNA Using Monophasic Lysis Reagents
A typical mammalian cell contains ∼10-5 µg of RNA, 80%-85% of which is ribosomal RNA (rRNA; chiefly the 28S, 18S, 5.8S, and 5S species). Most of the remaining 15%-20% consists of a variety of low-molecular-weight species (e.g., transfer RNAs [tRNAs] and small nuclear RNAs). These abundant RNAs are of defined size and sequence. In contrast, messenger RNA (mRNA), which makes up between 1% and 5% of the total cellular RNA, is heterogeneous in both size-from a few hundred bases to many kilobases in length-and sequence. In this introduction, the development and use of monophasic lysis reagents for the isolation of total RNA from eukaryotic cells is discussed.

kolgene
Serum Albumin, Lipid Free Protein |
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20-abx260049 | Abbexa |
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Bovine Serum Albumin, protease free |
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GK4012-1KG | Glentham Life Sciences | 1 kg | EUR 1165.2 |
Bovine Serum Albumin, protease free |
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GK4012-500G | Glentham Life Sciences | 500 g | EUR 638.4 |
PK15 Cell Serum-Free Medium |
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VCum-Lsx0007 | Creative Biolabs | 1 L | EUR 909.6 |
Description: PK15 cell serum-free medium has been specifically developed for suspension culture of PK-15 cells. |
CHO Cell Serum-Free Medium |
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VCum-Lsx0025 | Creative Biolabs | 1L | EUR 762 |
Description: A serum-free medium that is ideal for suspension culture of CHO cell lines (CHO DG44, CHO-K1 and CHO-S). |
HEK Cell Serum-Free Medium |
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VCum-Lsx0027 | Creative Biolabs | 1L | EUR 762 |
Description: A serum-free medium that is ideal for suspension culture of HEK cell lines. |
MDBK Cell Serum-Free Medium |
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VCum-Lsx0029 | Creative Biolabs | 1L | EUR 818.4 |
Description: Used in serum-free suspension MDBK cell line culture for bovine virus diarrhea or bovine rhinotracheitis vaccine production. |
Vero Cell Serum-Free Medium |
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VCum-Lsx0031 | Creative Biolabs | 1 L | EUR 909.6 |
Description: A serum-free medium that is ideal for suspension culture of Vero cell lines. |
500mL Insectagro DS2 Serum-Free |
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13-402-CV | Scientific Laboratory Supplies | PK6 | EUR 222.3 |
500mL INSECTAGRO SF9 serum free |
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13-410-CV | Scientific Laboratory Supplies | PK6 | EUR 192.66 |
EZ-DNA Reagents |
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BS8202 | Bio Basic | 100preps | EUR 105.67 |
EZ-DNA Reagents |
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SK8201 | Bio Basic | 100preps | EUR 112.2 |
Ecl Detection Reagents |
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RPN2105 | Scientific Laboratory Supplies | EACH | EUR 611.04 |
Ecl Detection Reagents |
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RPN3004 | Scientific Laboratory Supplies | EACH | EUR 489.06 |
Fetal Bovine Serum, Premium Tetracyclin Free |
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F0500-050 | GenDepot | 500ml | EUR 1388.4 |
B-27 Serum-Free Supplement (50x) |
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abx082466-10ml | Abbexa | 10 ml | EUR 393.6 |
Normal Bovine Serum (Protease/IgG free) |
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88R-1012 | Fitzgerald | 10 ml | EUR 218.4 |
Description: Normal Bovine Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
Bovine Serum Albumin, fatty acid free |
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GX5685-1KG | Glentham Life Sciences | 1 kg | EUR 1442.4 |
Bovine Serum Albumin, fatty acid free |
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GX5685-500G | Glentham Life Sciences | 500 g | EUR 792 |
ST Cell Serum-Free Medium-1 |
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VCum-Lsx0001 | Creative Biolabs | 1 L | EUR 888 |
Description: ST cell serum-free medium has been specifically developed for suspension culture of ST cells. |
ST Cell Serum-Free Medium-2 |
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VCum-Lsx0003 | Creative Biolabs | 1 L | EUR 909.6 |
Description: ST cell serum-free medium has been specifically developed for suspension culture of ST cells. |
PK15 Cell Serum-Free Medium (Powder) |
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VCum-Lsx0008-10L | Creative Biolabs | 10 L | EUR 2798.4 |
Description: PK15 cell serum-free medium has been specifically developed for suspension culture of PK-15 cells. |
PK15 Cell Serum-Free Medium (Powder) |
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VCum-Lsx0008-1L | Creative Biolabs | 1 L | EUR 333.6 |
Description: PK15 cell serum-free medium has been specifically developed for suspension culture of PK-15 cells. |
PK15 Cell Serum-Free Medium (Powder) |
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VCum-Lsx0008-5L | Creative Biolabs | 5 L | EUR 1429.2 |
Description: PK15 cell serum-free medium has been specifically developed for suspension culture of PK-15 cells. |
MDCK Cell Serum-Free Medium-1 |
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VCum-Lsx0009 | Creative Biolabs | 1 L | EUR 818.4 |
Description: MDCK cell serum-free medium has been specifically developed for suspension culture of MDCK cells. |
MDCK Cell Serum-Free Medium-2 |
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VCum-Lsx0011 | Creative Biolabs | 1 L | EUR 832.8 |
Description: Used in serum-free suspension MDCK cell line culture for avian influenza vaccine production. |
BHK Cell Serum-Free Medium-1 |
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VCum-Lsx0017 | Creative Biolabs | 1 L | EUR 776.4 |
Description: BHK cell serum-free medium has been specifically developed for suspension culture of BHK cells. |
BHK Cell Serum-Free Medium-2 |
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VCum-Lsx0019 | Creative Biolabs | 1 L | EUR 860.4 |
Description: Used in serum-free suspension BHK cell line culture for newcastle disease virus production. |
Insect Cell Serum-Free Medium-1 |
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VCum-Lsx0021 | Creative Biolabs | 1 L | EUR 888 |
Description: A serum-free medium that is ideal for suspension culture of insect cell lines (HighFive, SF9). |
Insect Cell Serum-Free Medium-2 |
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VCum-Lsx0023 | Creative Biolabs | 1 L | EUR 909.6 |
Description: A serum-free medium that is ideal for suspension culture of insect cell lines (HighFive, SF9). |
CHO Cell Serum-Free Medium (Powder) |
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VCum-Lsx0026-10L | Creative Biolabs | 10 L | EUR 2096.4 |
Description: A serum-free medium that is ideal for suspension culture of CHO cell lines (CHO DG44, CHO-K1 and CHO-S). |
CHO Cell Serum-Free Medium (Powder) |
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VCum-Lsx0026-1L | Creative Biolabs | 1 L | EUR 264 |
Description: A serum-free medium that is ideal for suspension culture of CHO cell lines (CHO DG44, CHO-K1 and CHO-S). |
CHO Cell Serum-Free Medium (Powder) |
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VCum-Lsx0026-5L | Creative Biolabs | 5 L | EUR 1077.6 |
Description: A serum-free medium that is ideal for suspension culture of CHO cell lines (CHO DG44, CHO-K1 and CHO-S). |
HEK Cell Serum-Free Medium (Powder) |
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VCum-Lsx0028 | Creative Biolabs | 50 L | EUR 7693.2 |
Description: A serum-free medium that is ideal for suspension culture of HEK cell lines. |
MDBK Cell Serum-Free Medium (Powder) |
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VCum-Lsx0030 | Creative Biolabs | 50 L | EUR 10309.2 |
Description: Used in serum-free suspension MDBK cell line culture for bovine virus diarrhea or bovine rhinotracheitis vaccine production. |
Vero Cell Serum-Free Medium (Powder) |
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VCum-Lsx0032-10L | Creative Biolabs | 10 L | EUR 2798.4 |
Description: A serum-free medium that is ideal for suspension culture of Vero cell lines. |
Vero Cell Serum-Free Medium (Powder) |
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VCum-Lsx0032-1L | Creative Biolabs | 1 L | EUR 338.4 |
Description: A serum-free medium that is ideal for suspension culture of Vero cell lines. |
Vero Cell Serum-Free Medium (Powder) |
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VCum-Lsx0032-5L | Creative Biolabs | 5 L | EUR 1449.6 |
Description: A serum-free medium that is ideal for suspension culture of Vero cell lines. |
Corning Lymphocyte Serum Free Medium KBM551 |
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88-551-CM | Scientific Laboratory Supplies | 1L | EUR 182.4 |
Corning Lymphocyte Serum Free Medium KBM581 |
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88-581-CM | Scientific Laboratory Supplies | 1L | EUR 240.54 |
REAGENTS HIGH RANGE AMMONIA |
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HI73325 | Scientific Laboratory Supplies | PK25 | EUR 38.19 |
High Range Ammonia Reagents |
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HI9373301 | Scientific Laboratory Supplies | EACH | EUR 91.2 |
Alkphos Direct Labelling Reagents |
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RPN3680 | Scientific Laboratory Supplies | EACH | EUR 686.28 |
Human Apolipoprotein AI / B Free Serum Protein |
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abx060869-100ml | Abbexa | 100 ml | EUR 594 |
ST Cell Serum-Free Medium-1 (Powder) |
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VCum-Lsx0002-10L | Creative Biolabs | 10 L | EUR 2658 |
Description: ST cell serum-free medium has been specifically developed for suspension culture of ST cells. |
ST Cell Serum-Free Medium-1 (Powder) |
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VCum-Lsx0002-1L | Creative Biolabs | 1 L | EUR 319.2 |
Description: ST cell serum-free medium has been specifically developed for suspension culture of ST cells. |
ST Cell Serum-Free Medium-1 (Powder) |
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VCum-Lsx0002-5L | Creative Biolabs | 5 L | EUR 1358.4 |
Description: ST cell serum-free medium has been specifically developed for suspension culture of ST cells. |
ST Cell Serum-Free Medium-2 (Powder) |
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VCum-Lsx0004-10L | Creative Biolabs | 10 L | EUR 2798.4 |
Description: ST cell serum-free medium has been specifically developed for suspension culture of ST cells. |
ST Cell Serum-Free Medium-2 (Powder) |
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VCum-Lsx0004-1L | Creative Biolabs | 1 L | EUR 333.6 |
Description: ST cell serum-free medium has been specifically developed for suspension culture of ST cells. |
ST Cell Serum-Free Medium-2 (Powder) |
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VCum-Lsx0004-5L | Creative Biolabs | 5 L | EUR 1429.2 |
Description: ST cell serum-free medium has been specifically developed for suspension culture of ST cells. |
MDCK Cell Serum-Free Medium-1 (Powder) |
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VCum-Lsx0010-10L | Creative Biolabs | 10 L | EUR 2236.8 |
Description: MDCK cell serum-free medium has been specifically developed for suspension culture of MDCK cells. |
MDCK Cell Serum-Free Medium-1 (Powder) |
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VCum-Lsx0010-1L | Creative Biolabs | 1 L | EUR 277.2 |
Description: MDCK cell serum-free medium has been specifically developed for suspension culture of MDCK cells. |
MDCK Cell Serum-Free Medium-1 (Powder) |
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VCum-Lsx0010-5L | Creative Biolabs | 5 L | EUR 1148.4 |
Description: MDCK cell serum-free medium has been specifically developed for suspension culture of MDCK cells. |
MDCK Cell Serum-Free Medium-2 (Powder) |
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VCum-Lsx0012-10L | Creative Biolabs | 10 L | EUR 2306.4 |
Description: Used in serum-free suspension MDCK cell line culture for avian influenza vaccine production. |
MDCK Cell Serum-Free Medium-2 (Powder) |
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VCum-Lsx0012-1L | Creative Biolabs | 1 L | EUR 284.4 |
Description: Used in serum-free suspension MDCK cell line culture for avian influenza vaccine production. |
MDCK Cell Serum-Free Medium-2 (Powder) |
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VCum-Lsx0012-5L | Creative Biolabs | 5 L | EUR 1183.2 |
Description: Used in serum-free suspension MDCK cell line culture for avian influenza vaccine production. |
BHK Cell Serum-Free Medium-1 (Powder) |
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VCum-Lsx0018-10L | Creative Biolabs | 10 L | EUR 2025.6 |
Description: BHK cellserum-free medium has been specifically developed for suspension culture of BHK cells. |
BHK Cell Serum-Free Medium-1 (Powder) |
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VCum-Lsx0018-1L | Creative Biolabs | 1 L | EUR 256.8 |
Description: BHK cellserum-free medium has been specifically developed for suspension culture of BHK cells. |
BHK Cell Serum-Free Medium-1 (Powder) |
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VCum-Lsx0018-5L | Creative Biolabs | 5 L | EUR 1042.8 |
Description: BHK cellserum-free medium has been specifically developed for suspension culture of BHK cells. |
BHK Cell Serum-Free Medium-2 (Powder) |
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VCum-Lsx0020-10L | Creative Biolabs | 10 L | EUR 2306.4 |
Description: Used in serum-free suspension BHK cell line culture for newcastle disease virus production. |
BHK Cell Serum-Free Medium-2 (Powder) |
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VCum-Lsx0020-1L | Creative Biolabs | 1 L | EUR 284.4 |
Description: Used in serum-free suspension BHK cell line culture for newcastle disease virus production. |
BHK Cell Serum-Free Medium-2 (Powder) |
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VCum-Lsx0020-5L | Creative Biolabs | 5 L | EUR 1183.2 |
Description: Used in serum-free suspension BHK cell line culture for newcastle disease virus production. |
Insect Cell Serum-Free Medium-1 (Powder) |
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VCum-Lsx0022-10L | Creative Biolabs | 10 L | EUR 2658 |
Description: A serum-free medium that is ideal for suspension culture of insect cell lines (HighFive, SF9). |
Insect Cell Serum-Free Medium-1 (Powder) |
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VCum-Lsx0022-1L | Creative Biolabs | 1 L | EUR 319.2 |
Description: A serum-free medium that is ideal for suspension culture of insect cell lines (HighFive, SF9). |
Insect Cell Serum-Free Medium-1 (Powder) |
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VCum-Lsx0022-5L | Creative Biolabs | 5 L | EUR 1358.4 |
Description: A serum-free medium that is ideal for suspension culture of insect cell lines (HighFive, SF9). |
Insect Cell Serum-Free Medium-2 (Powder) |
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VCum-Lsx0024-10L | Creative Biolabs | 10 L | EUR 2798.4 |
Description: A serum-free medium that is ideal for suspension culture of insect cell lines (HighFive, SF9). |
Insect Cell Serum-Free Medium-2 (Powder) |
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VCum-Lsx0024-1L | Creative Biolabs | 1 L | EUR 333.6 |
Description: A serum-free medium that is ideal for suspension culture of insect cell lines (HighFive, SF9). |
Insect Cell Serum-Free Medium-2 (Powder) |
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VCum-Lsx0024-5L | Creative Biolabs | 5 L | EUR 1429.2 |
Description: A serum-free medium that is ideal for suspension culture of insect cell lines (HighFive, SF9). |
CryoProtX Mix Reagents 1.5 mL |
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M-MDSR-61 | MiTeGen | 1.5 ml ml | EUR 37 |
Description: CryoProtX Mix Reagents 1.5 mL |
Set of 10 Biolipidure Reagents |
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Biolipidure-set | AS ONE International | 10mLx10 | EUR 1820.4 |
Description: Set of 10 Biolipidure Reagents, whose applications include Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
Bovine serum albumin (BSA) protein (>99%, Protease-free) |
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BSA16-N-100 | Alpha Diagnostics | 100 mg | EUR 343.2 |
Insect Cell Medium: Serum-Free Insect Culture Medium |
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ABP-MED-10002 | Allele Biotech | 1 liter | Ask for price |
HSA Human Serum Albumin Recombinant Protein, Lipid Free |
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PROTP02768-3 | BosterBio | Regular: 50mg | EUR 565.2 |
Description: HSA Human Recombinant lipid reduced produced in Plant is a non-glycosylated, polypeptide chain containing 585 amino acids and having a molecular mass of 67 kDa. |
Exo-Flow 2.0 Basic Kit without antibody (Streptavidin beads + reagents) - for Serum or Plasma |
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EXOFLOW2-BASICA-SP | SBI | 30 rxn | EUR 1201.2 |
Mouse Serum |
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abx098261-100L | Abbexa | 100 L | EUR 155407.2 |
Mouse Serum |
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20-abx098261 | Abbexa |
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Mouse Serum |
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abx098261-5L | Abbexa | 5 L | EUR 10599.6 |
Mouse Serum |
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abx098262-1ml | Abbexa | 1 ml | EUR 2064 |
Bradford(Coomassie) Protein Assay Plus Reagents |
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P7201-050 | GenDepot | 450ml | EUR 225.6 |
CryoProtX Mix Eco Reagents 1.5 mL |
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M-MDSR-61-ECO | MiTeGen | 1.5 ml ml | EUR 37 |
Description: CryoProtX Mix Eco Reagents 1.5 mL |
Purified rat serum albumin protein (RSA, >99%, globulin free) |
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ALBR13-N-10 | Alpha Diagnostics | 10 mg | EUR 343.2 |
Human Male AB Serum (Converted from Plasma), Xeno-Free |
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AR1005-0100 | Akron Biotech | 100mL | Ask for price |
Rabbit control serum (non-immunized, Disease free, SPF Rabbits) |
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NSPF-1 | Alpha Diagnostics | 1 ml | EUR 343.2 |
Rabbit control serum (non-immunized, Disease free, SPF Rabbits) |
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NSPF-10 | Alpha Diagnostics | 10 ml | EUR 781.2 |
Rabbit control serum (non-immunized, Disease free, SPF Rabbits) |
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NSPF-5 | Alpha Diagnostics | 5 ml | EUR 634.8 |
Bovine Serum Albumin ? Heat Shock, Protease Free, pH 7.0 |
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7919-100 | Biovision | each | EUR 783.6 |
Bovine Serum Albumin ? Heat Shock, Protease Free, pH 7.0 |
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7919-25 | Biovision | each | EUR 294 |
Bovine Serum Albumin ? Heat Shock, Protease Free, pH 7.0 |
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7919-5 | Biovision | each | EUR 144 |
Human IgG (serum origin, purified >97%, low endotoxin, azide free) |
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20007-1-LE-1 | Alpha Diagnostics | 1 mg | EUR 196.8 |
Bovine Serum Albumin ? Heat Shock, Protease DNASE Free, pH 7.0 |
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7920-100 | Biovision | each | EUR 1096.8 |
Bovine Serum Albumin ? Heat Shock, Protease DNASE Free, pH 7.0 |
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7920-1000 | Biovision | each | Ask for price |
Bovine Serum Albumin ? Heat Shock, Protease DNASE Free, pH 7.0 |
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7920-25 | Biovision | each | EUR 444 |
Bovine Serum Albumin ? Heat Shock, Protease DNASE Free, pH 7.0 |
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7920-5 | Biovision | each | EUR 183.6 |
Bovine Serum Albumin ? Heat Shock, Fatty Acid Free, pH 7.0 |
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7921-100 | Biovision | each | EUR 1358.4 |
Bovine Serum Albumin ? Heat Shock, Fatty Acid Free, pH 7.0 |
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7921-25 | Biovision | each | EUR 535.2 |
Bovine Serum Albumin ? Heat Shock, Fatty Acid Free, pH 7.0 |
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7921-5 | Biovision | each | EUR 210 |
Mouse Serum Albumin |
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30R-3304 | Fitzgerald | 10 mg | EUR 224.4 |
Description: Purified native Mouse Serum Albumin |
Mouse Complement Serum |
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32R-CM001 | Fitzgerald | 5 ml | EUR 522 |
Description: Frozen high activity mouse complement serum |
Normal Mouse Serum |
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88-NM35 | Fitzgerald | 50 ml | EUR 619.2 |
Description: Normal Mouse Serum |
Normal Mouse Serum |
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88R-1002 | Fitzgerald | 5 ml | EUR 243.6 |
Description: Normal Mouse Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
Mouse Free Tri-iodothyronine, Free-T3 ELISA Kit |
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CSB-E05077m-24T | Cusabio | 1 plate of 24 wells | EUR 198 |
Description: Quantitative competitive ELISA kit for measuring Mouse Free Tri-iodothyronine, Free-T3 in samples from serum, plasma, cell culture supernates, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price. |
Mouse Free Tri-iodothyronine, Free-T3 ELISA Kit |
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1-CSB-E05077m | Cusabio |
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Description: Quantitative competitive ELISA kit for measuring Mouse Free Tri-iodothyronine, Free-T3 in samples from serum, plasma, cell culture supernates, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits. |
Human IgG-Biotin (serum origin, purified >97%, low endotoxin, azide free) |
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20007-1-LE-BTN | Alpha Diagnostics | 100 ug | EUR 343.2 |
Human Male AB Serum (Converted from Plasma), Xeno-Free Heat Inactivated |
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AR1006-0100 | Akron Biotech | 100mL | Ask for price |
Bovine Serum Albumin ? Cohn Fraction V, Fatty Acid Free, pH ? 7.0 |
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7907-100 | Biovision | each | EUR 783.6 |
Bovine Serum Albumin ? Cohn Fraction V, Fatty Acid Free, pH ? 7.0 |
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7907-25 | Biovision | each | EUR 294 |
Bovine Serum Albumin ? Cohn Fraction V, Fatty Acid Free, pH ? 7.0 |
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7907-5 | Biovision | each | EUR 144 |
Bovine Serum Albumin ? Cohn Fraction V, Fatty Acid Free, pH ? 5.2 |
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7908-100 | Biovision | each | EUR 783.6 |
Bovine Serum Albumin ? Cohn Fraction V, Fatty Acid Free, pH ? 5.2 |
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7908-25 | Biovision | each | EUR 294 |
Bovine Serum Albumin ? Cohn Fraction V, Fatty Acid Free, pH ? 5.2 |
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7908-5 | Biovision | each | EUR 144 |
Mouse Serum Amyloid P Component (SAP) Reference serum |
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SAP12-RS | Alpha Diagnostics | 100 ul | EUR 388.8 |
Mouse Free Tri-iodothyronine Indes,Free-T3 ELISA Kit |
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CN-02860M1 | ChemNorm | 96T | EUR 571.2 |
Mouse Free Tri-iodothyronine Indes,Free-T3 ELISA Kit |
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CN-02860M2 | ChemNorm | 48T | EUR 391.2 |
Mouse Free Tri-iodothyronine Indes(Free-T3)ELISA Kit |
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GA-E0078MS-48T | GenAsia Biotech | 48T | EUR 403.2 |
Mouse Free Tri-iodothyronine Indes(Free-T3)ELISA Kit |
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GA-E0078MS-96T | GenAsia Biotech | 96T | EUR 640.8 |
Mouse Free Tri-iodothyronine Indes(Free-T3)ELISA Kit |
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QY-E20615 | Qayee Biotechnology | 96T | EUR 433.2 |
Mouse Free Tri-iodothyronine Indes,Free-T3 ELISA Kit |
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YLA0389MO-48T | Shanghai YL Biotech | 48T | EUR 465 |
Mouse Free Tri-iodothyronine Indes,Free-T3 ELISA Kit |
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YLA0389MO-96T | Shanghai YL Biotech | 96T | EUR 600 |
Mouse Serum albumin (Alb) |
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1-CSB-YP001561MO | Cusabio |
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Description: Recombinant Mouse Serum albumin(Alb) expressed in Yeast |
Mouse Serum albumin (Alb) |
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1-CSB-BP001561MO | Cusabio |
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Description: Recombinant Mouse Serum albumin(Alb) expressed in Baculovirus |
Mouse Serum Albumin antibody |
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70R-AG001 | Fitzgerald | 1 mg | EUR 307.2 |
Description: Affinity purified Goat polyclonal Mouse Serum Albumin antibody |
Mouse Serum Albumin antibody |
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20R-AR005 | Fitzgerald | 20 mg | EUR 392.4 |
Description: Rabbit polyclonal Mouse Serum Albumin antibody |
Mouse Serum albumin antibody |
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20R-AR041 | Fitzgerald | 2 ml | EUR 354 |
Description: Rabbit polyclonal Mouse Serum albumin antibody |
Mouse Serum Albumin protein |
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30-1179 | Fitzgerald | 10 mg | EUR 283.2 |
Description: Purified native Mouse Albumin protein |
Mouse Serum Albumin Protein |
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20-abx600021 | Abbexa |
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Non-sterile mouse serum |
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MS05-0050 | Equitech | 50 ml | EUR 277.68 |
Non-sterile mouse serum |
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MS05-0100 | Equitech | 100 ml | EUR 308.88 |
Non-sterile mouse serum |
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MS05-0500 | Equitech | 500 ml | EUR 528.84 |
IPTG, Animal-Free (Dioxane-Free) |
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1649-1G | Biovision | each | EUR 138 |
IPTG, Animal-Free (Dioxane-Free) |
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1649-50G | Biovision | each | EUR 1182 |
IPTG, Animal-Free (Dioxane-Free) |
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1649-5G | Biovision | each | EUR 261.6 |
Purified rat serum albumin protein (RSA, >99%, fatty acid and globulin free) |
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ALBR14-N-10 | Alpha Diagnostics | 10 mg | EUR 416.4 |
Bovine Serum Albumin ? Cohn Fraction V, Immunoassay Grade, Protease Free, pH ? 7.0 |
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7906-100 | Biovision | each | EUR 783.6 |
Bovine Serum Albumin ? Cohn Fraction V, Immunoassay Grade, Protease Free, pH ? 7.0 |
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7906-25 | Biovision | each | EUR 294 |
Bovine Serum Albumin ? Cohn Fraction V, Immunoassay Grade, Protease Free, pH ? 7.0 |
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7906-5 | Biovision | each | EUR 144 |
Bovine Serum Albumin ? Cohn Fraction V, Immunoassay Grade, Protease Free, pH ? 5.2 |
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7911-100 | Biovision | each | EUR 783.6 |
Bovine Serum Albumin ? Cohn Fraction V, Immunoassay Grade, Protease Free, pH ? 5.2 |
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7911-25 | Biovision | each | EUR 294 |
Bovine Serum Albumin ? Cohn Fraction V, Immunoassay Grade, Protease Free, pH ? 5.2 |
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7911-5 | Biovision | each | EUR 144 |
Amersham ECL Western Blotting Detection Reagents for 4000cm2 membrane |
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RPN2106 | Scientific Laboratory Supplies | EACH | EUR 314.64 |
Amersham ECL Western Blotting Detection Reagents for 1000cm2 membrane |
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RPN2109 | Scientific Laboratory Supplies | EACH | EUR 158.46 |
Amersham ECL Western Blotting Detection Reagents for 6000cm2 membrane |
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RPN2134 | Scientific Laboratory Supplies | 1KIT | EUR 482.22 |
Amersham ECL Western Blotting Detection Reagents for 2000cm2 membrane |
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RPN2209 | Scientific Laboratory Supplies | EACH | EUR 245.1 |
Mouse Free cholesterol ELISA kit |
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E03F0212-192T | BlueGene | 192 tests | EUR 1524 |
Description: A competitive ELISA for quantitative measurement of Mouse Free cholesterol in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Mouse Free cholesterol ELISA kit |
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E03F0212-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
Description: A competitive ELISA for quantitative measurement of Mouse Free cholesterol in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Mouse Free cholesterol ELISA kit |
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E03F0212-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
Description: A competitive ELISA for quantitative measurement of Mouse Free cholesterol in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Cobalt-CatalyzedDefluorosilylation of Aryl Fluorides via Grignard Reagent Formation
Transition-metal-catalyzed transformations of the carbon-fluorine bond not only tackle an interesting problem of challenging bond activation but also offer new synthetic strategies where the relatively inert C-F bond is converted to versatile functional groups. Herein we report a practical cobalt-catalyzedsilylation of aryl fluorides that uses a cheap electrophilic silicon source with magnesium. This method is compatible with various silicon sources and can be operated under aerobic conditions. Mechanistic studies support the in situ formation of a Grignard reagent, which is captured by the electrophilic silicon source.
A DAPI-Based Reagent for Mass Cytometry
Multiparametric single cell analysis has seen dramatic improvements with the introduction of Mass Cytometry (MC) and Imaging Mass Cytometry (IMC™). These technologies expanded the number of biomarkers that can be identified simultaneously by using heavy isotope-tagged antibody reagents. Small molecule probes bearing heavy isotopes are emerging as additional useful functional reporters of cellular features.
Realizing this, we explored the iodination of DAPI to produce a heavy atom substituted derivative of the commonly used fluorescent DNA stain. Although exhibiting a drastically reduced fluorescence emission profile, I-DAPI retains strong binding affinity for DNA. I-DAPI was used to detect cellular DNA in MC and IMC™ assays with comparable efficiency to known Ir containing DNA intercalators. This work suggests repurposing of well-known colorimetric stains through simple reactions could be an effective strategy to develop new, functional MC and IMC™ reagents.
Cautionary note on contamination of reagents used for molecular detection of SARS-CoV-2
Reverse transcription (RT)-PCR, the principal diagnostic method applied in the world-wide struggle against COVID-19, is capable of detecting a single molecule of a viral genome. Correctly designed and practiced RT-PCR assays for SARS-CoV-2 should not cross react with similar but distinct viral pathogens, such as the coronaviruses associated with the common cold, and should perform with very high analytical sensitivity. This analytical performance is predicated on the ability of the method to detect the presence of the selected nucleic acid target, without detection of a false positive signal.
Influence of reagent lots and multiple measuring systems on estimating the coefficient of variation from quality control data; implications for uncertainty estimation and interpretation of QC results
- AbstractBackgroundClinical laboratories use internal quality control (QC) data to calculate standard deviation (SD) and coefficient of variation (CV) to estimate uncertainty of results and to interpret QC results. We examined the influence of different instruments, and QC and reagent lots on the CV calculated from QC data.MethodsResults for BioRadMultiqual frozen liquid QC samples over a 2-year interval were partitioned by QC and reagent lots.
- The mean and CV were calculated for each partition for each of three Abbott Architect c8000 instruments for measuring serum alanine amino transferase (ALT), creatinine (enzymatic), glucose and sodium.ResultsCVs differed among partitions and instruments for two QC levels by 5.8- and 3.3-fold for ALT, by 4.7- and 2.1-fold for creatinine, by 2.0- and 2.6-fold for glucose, and by 2.1- and 2.0-fold for sodium. Pooled CVs for two QC levels varied among instruments by 1.78- and 1.11-fold for ALT, by 1.63- and 1.11-fold for creatinine, by 1.08- and 1.06-fold for glucose, and by 1.24- and 1.31-fold for sodium.
- ConclusionsThe CVs from QC data varied substantially among QC and reagent lots and for different identical specification instruments. The CV used to estimate uncertainty for a measurement result or as the basis for interpreting individual QC results must be derived over a sufficient time interval to obtain a pooled CV that represents “typical” performance of a measuring system. An estimate of uncertainty provided to users of laboratory results will itself have uncertainty that can influence medical decisions.
Human Free PSA (f-PSA) ELISA Kit |
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PRB-5049-FREE-5 | Cell Biolabs | 5 x 96 assays | EUR 2739.6 |
Human Serum (Troponin I Free) |
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90R-106 | Fitzgerald | 100 ml | EUR 1417.2 |
Description: Troponion I free normal human serum |
Serum Albumin, Lipid Free Protein |
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20-abx260049 | Abbexa |
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Bovine Serum Albumin, protease free |
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GK4012-1KG | Glentham Life Sciences | 1 kg | EUR 1165.2 |
Bovine Serum Albumin, protease free |
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GK4012-500G | Glentham Life Sciences | 500 g | EUR 638.4 |
PK15 Cell Serum-Free Medium |
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VCum-Lsx0007 | Creative Biolabs | 1 L | EUR 909.6 |
Description: PK15 cell serum-free medium has been specifically developed for suspension culture of PK-15 cells. |
CHO Cell Serum-Free Medium |
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VCum-Lsx0025 | Creative Biolabs | 1L | EUR 762 |
Description: A serum-free medium that is ideal for suspension culture of CHO cell lines (CHO DG44, CHO-K1 and CHO-S). |
HEK Cell Serum-Free Medium |
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VCum-Lsx0027 | Creative Biolabs | 1L | EUR 762 |
Description: A serum-free medium that is ideal for suspension culture of HEK cell lines. |
MDBK Cell Serum-Free Medium |
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VCum-Lsx0029 | Creative Biolabs | 1L | EUR 818.4 |
Description: Used in serum-free suspension MDBK cell line culture for bovine virus diarrhea or bovine rhinotracheitis vaccine production. |
Vero Cell Serum-Free Medium |
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VCum-Lsx0031 | Creative Biolabs | 1 L | EUR 909.6 |
Description: A serum-free medium that is ideal for suspension culture of Vero cell lines. |
500mL Insectagro DS2 Serum-Free |
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13-402-CV | Scientific Laboratory Supplies | PK6 | EUR 222.3 |
500mL INSECTAGRO SF9 serum free |
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13-410-CV | Scientific Laboratory Supplies | PK6 | EUR 192.66 |
EZ-DNA Reagents |
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BS8202 | Bio Basic | 100preps | EUR 105.67 |
EZ-DNA Reagents |
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SK8201 | Bio Basic | 100preps | EUR 112.2 |
Ecl Detection Reagents |
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RPN2105 | Scientific Laboratory Supplies | EACH | EUR 611.04 |
Ecl Detection Reagents |
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RPN3004 | Scientific Laboratory Supplies | EACH | EUR 489.06 |
B-27 Serum-Free Supplement (50x) |
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abx082466-10ml | Abbexa | 10 ml | EUR 393.6 |
Normal Bovine Serum (Protease/IgG free) |
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88R-1012 | Fitzgerald | 10 ml | EUR 218.4 |
Description: Normal Bovine Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
Fetal Bovine Serum, Premium Tetracyclin Free |
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F0500-050 | GenDepot | 500ml | EUR 1388.4 |
Bovine Serum Albumin, fatty acid free |
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GX5685-1KG | Glentham Life Sciences | 1 kg | EUR 1442.4 |
Bovine Serum Albumin, fatty acid free |
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GX5685-500G | Glentham Life Sciences | 500 g | EUR 792 |
ST Cell Serum-Free Medium-1 |
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VCum-Lsx0001 | Creative Biolabs | 1 L | EUR 888 |
Description: ST cell serum-free medium has been specifically developed for suspension culture of ST cells. |
ST Cell Serum-Free Medium-2 |
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VCum-Lsx0003 | Creative Biolabs | 1 L | EUR 909.6 |
Description: ST cell serum-free medium has been specifically developed for suspension culture of ST cells. |
PK15 Cell Serum-Free Medium (Powder) |
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VCum-Lsx0008-10L | Creative Biolabs | 10 L | EUR 2798.4 |
Description: PK15 cell serum-free medium has been specifically developed for suspension culture of PK-15 cells. |
PK15 Cell Serum-Free Medium (Powder) |
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VCum-Lsx0008-1L | Creative Biolabs | 1 L | EUR 333.6 |
Description: PK15 cell serum-free medium has been specifically developed for suspension culture of PK-15 cells. |
PK15 Cell Serum-Free Medium (Powder) |
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VCum-Lsx0008-5L | Creative Biolabs | 5 L | EUR 1429.2 |
Description: PK15 cell serum-free medium has been specifically developed for suspension culture of PK-15 cells. |
MDCK Cell Serum-Free Medium-1 |
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VCum-Lsx0009 | Creative Biolabs | 1 L | EUR 818.4 |
Description: MDCK cell serum-free medium has been specifically developed for suspension culture of MDCK cells. |
MDCK Cell Serum-Free Medium-2 |
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VCum-Lsx0011 | Creative Biolabs | 1 L | EUR 832.8 |
Description: Used in serum-free suspension MDCK cell line culture for avian influenza vaccine production. |
BHK Cell Serum-Free Medium-1 |
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VCum-Lsx0017 | Creative Biolabs | 1 L | EUR 776.4 |
Description: BHK cell serum-free medium has been specifically developed for suspension culture of BHK cells. |
BHK Cell Serum-Free Medium-2 |
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VCum-Lsx0019 | Creative Biolabs | 1 L | EUR 860.4 |
Description: Used in serum-free suspension BHK cell line culture for newcastle disease virus production. |
Insect Cell Serum-Free Medium-1 |
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VCum-Lsx0021 | Creative Biolabs | 1 L | EUR 888 |
Description: A serum-free medium that is ideal for suspension culture of insect cell lines (HighFive, SF9). |
Insect Cell Serum-Free Medium-2 |
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VCum-Lsx0023 | Creative Biolabs | 1 L | EUR 909.6 |
Description: A serum-free medium that is ideal for suspension culture of insect cell lines (HighFive, SF9). |
CHO Cell Serum-Free Medium (Powder) |
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VCum-Lsx0026-10L | Creative Biolabs | 10 L | EUR 2096.4 |
Description: A serum-free medium that is ideal for suspension culture of CHO cell lines (CHO DG44, CHO-K1 and CHO-S). |
CHO Cell Serum-Free Medium (Powder) |
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VCum-Lsx0026-1L | Creative Biolabs | 1 L | EUR 264 |
Description: A serum-free medium that is ideal for suspension culture of CHO cell lines (CHO DG44, CHO-K1 and CHO-S). |
CHO Cell Serum-Free Medium (Powder) |
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VCum-Lsx0026-5L | Creative Biolabs | 5 L | EUR 1077.6 |
Description: A serum-free medium that is ideal for suspension culture of CHO cell lines (CHO DG44, CHO-K1 and CHO-S). |
HEK Cell Serum-Free Medium (Powder) |
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VCum-Lsx0028 | Creative Biolabs | 50 L | EUR 7693.2 |
Description: A serum-free medium that is ideal for suspension culture of HEK cell lines. |
MDBK Cell Serum-Free Medium (Powder) |
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VCum-Lsx0030 | Creative Biolabs | 50 L | EUR 10309.2 |
Description: Used in serum-free suspension MDBK cell line culture for bovine virus diarrhea or bovine rhinotracheitis vaccine production. |
Vero Cell Serum-Free Medium (Powder) |
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VCum-Lsx0032-10L | Creative Biolabs | 10 L | EUR 2798.4 |
Description: A serum-free medium that is ideal for suspension culture of Vero cell lines. |
Vero Cell Serum-Free Medium (Powder) |
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VCum-Lsx0032-1L | Creative Biolabs | 1 L | EUR 338.4 |
Description: A serum-free medium that is ideal for suspension culture of Vero cell lines. |
Vero Cell Serum-Free Medium (Powder) |
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VCum-Lsx0032-5L | Creative Biolabs | 5 L | EUR 1449.6 |
Description: A serum-free medium that is ideal for suspension culture of Vero cell lines. |
Corning Lymphocyte Serum Free Medium KBM551 |
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88-551-CM | Scientific Laboratory Supplies | 1L | EUR 182.4 |
Corning Lymphocyte Serum Free Medium KBM581 |
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88-581-CM | Scientific Laboratory Supplies | 1L | EUR 240.54 |
REAGENTS HIGH RANGE AMMONIA |
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HI73325 | Scientific Laboratory Supplies | PK25 | EUR 38.19 |
High Range Ammonia Reagents |
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HI9373301 | Scientific Laboratory Supplies | EACH | EUR 91.2 |
Alkphos Direct Labelling Reagents |
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RPN3680 | Scientific Laboratory Supplies | EACH | EUR 686.28 |
Purified rat serum albumin protein (RSA, >99%, globulin free) |
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ALBR13-N-10 | Alpha Diagnostics | 10 mg | EUR 343.2 |
Human Apolipoprotein AI / B Free Serum Protein |
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abx060869-100ml | Abbexa | 100 ml | EUR 594 |
ST Cell Serum-Free Medium-1 (Powder) |
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VCum-Lsx0002-10L | Creative Biolabs | 10 L | EUR 2658 |
Description: ST cell serum-free medium has been specifically developed for suspension culture of ST cells. |
ST Cell Serum-Free Medium-1 (Powder) |
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VCum-Lsx0002-1L | Creative Biolabs | 1 L | EUR 319.2 |
Description: ST cell serum-free medium has been specifically developed for suspension culture of ST cells. |
ST Cell Serum-Free Medium-1 (Powder) |
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VCum-Lsx0002-5L | Creative Biolabs | 5 L | EUR 1358.4 |
Description: ST cell serum-free medium has been specifically developed for suspension culture of ST cells. |
ST Cell Serum-Free Medium-2 (Powder) |
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VCum-Lsx0004-10L | Creative Biolabs | 10 L | EUR 2798.4 |
Description: ST cell serum-free medium has been specifically developed for suspension culture of ST cells. |
ST Cell Serum-Free Medium-2 (Powder) |
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VCum-Lsx0004-1L | Creative Biolabs | 1 L | EUR 333.6 |
Description: ST cell serum-free medium has been specifically developed for suspension culture of ST cells. |
ST Cell Serum-Free Medium-2 (Powder) |
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VCum-Lsx0004-5L | Creative Biolabs | 5 L | EUR 1429.2 |
Description: ST cell serum-free medium has been specifically developed for suspension culture of ST cells. |
MDCK Cell Serum-Free Medium-1 (Powder) |
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VCum-Lsx0010-10L | Creative Biolabs | 10 L | EUR 2236.8 |
Description: MDCK cell serum-free medium has been specifically developed for suspension culture of MDCK cells. |
MDCK Cell Serum-Free Medium-1 (Powder) |
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VCum-Lsx0010-1L | Creative Biolabs | 1 L | EUR 277.2 |
Description: MDCK cell serum-free medium has been specifically developed for suspension culture of MDCK cells. |
MDCK Cell Serum-Free Medium-1 (Powder) |
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VCum-Lsx0010-5L | Creative Biolabs | 5 L | EUR 1148.4 |
Description: MDCK cell serum-free medium has been specifically developed for suspension culture of MDCK cells. |
MDCK Cell Serum-Free Medium-2 (Powder) |
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VCum-Lsx0012-10L | Creative Biolabs | 10 L | EUR 2306.4 |
Description: Used in serum-free suspension MDCK cell line culture for avian influenza vaccine production. |
MDCK Cell Serum-Free Medium-2 (Powder) |
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VCum-Lsx0012-1L | Creative Biolabs | 1 L | EUR 284.4 |
Description: Used in serum-free suspension MDCK cell line culture for avian influenza vaccine production. |
MDCK Cell Serum-Free Medium-2 (Powder) |
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VCum-Lsx0012-5L | Creative Biolabs | 5 L | EUR 1183.2 |
Description: Used in serum-free suspension MDCK cell line culture for avian influenza vaccine production. |
BHK Cell Serum-Free Medium-1 (Powder) |
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VCum-Lsx0018-10L | Creative Biolabs | 10 L | EUR 2025.6 |
Description: BHK cellserum-free medium has been specifically developed for suspension culture of BHK cells. |
BHK Cell Serum-Free Medium-1 (Powder) |
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VCum-Lsx0018-1L | Creative Biolabs | 1 L | EUR 256.8 |
Description: BHK cellserum-free medium has been specifically developed for suspension culture of BHK cells. |
BHK Cell Serum-Free Medium-1 (Powder) |
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VCum-Lsx0018-5L | Creative Biolabs | 5 L | EUR 1042.8 |
Description: BHK cellserum-free medium has been specifically developed for suspension culture of BHK cells. |
BHK Cell Serum-Free Medium-2 (Powder) |
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VCum-Lsx0020-10L | Creative Biolabs | 10 L | EUR 2306.4 |
Description: Used in serum-free suspension BHK cell line culture for newcastle disease virus production. |
BHK Cell Serum-Free Medium-2 (Powder) |
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VCum-Lsx0020-1L | Creative Biolabs | 1 L | EUR 284.4 |
Description: Used in serum-free suspension BHK cell line culture for newcastle disease virus production. |
BHK Cell Serum-Free Medium-2 (Powder) |
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VCum-Lsx0020-5L | Creative Biolabs | 5 L | EUR 1183.2 |
Description: Used in serum-free suspension BHK cell line culture for newcastle disease virus production. |
Insect Cell Serum-Free Medium-1 (Powder) |
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VCum-Lsx0022-10L | Creative Biolabs | 10 L | EUR 2658 |
Description: A serum-free medium that is ideal for suspension culture of insect cell lines (HighFive, SF9). |
Insect Cell Serum-Free Medium-1 (Powder) |
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VCum-Lsx0022-1L | Creative Biolabs | 1 L | EUR 319.2 |
Description: A serum-free medium that is ideal for suspension culture of insect cell lines (HighFive, SF9). |
Insect Cell Serum-Free Medium-1 (Powder) |
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VCum-Lsx0022-5L | Creative Biolabs | 5 L | EUR 1358.4 |
Description: A serum-free medium that is ideal for suspension culture of insect cell lines (HighFive, SF9). |
Insect Cell Serum-Free Medium-2 (Powder) |
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VCum-Lsx0024-10L | Creative Biolabs | 10 L | EUR 2798.4 |
Description: A serum-free medium that is ideal for suspension culture of insect cell lines (HighFive, SF9). |
Insect Cell Serum-Free Medium-2 (Powder) |
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VCum-Lsx0024-1L | Creative Biolabs | 1 L | EUR 333.6 |
Description: A serum-free medium that is ideal for suspension culture of insect cell lines (HighFive, SF9). |
Insect Cell Serum-Free Medium-2 (Powder) |
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VCum-Lsx0024-5L | Creative Biolabs | 5 L | EUR 1429.2 |
Description: A serum-free medium that is ideal for suspension culture of insect cell lines (HighFive, SF9). |
CryoProtX Mix Reagents 1.5 mL |
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M-MDSR-61 | MiTeGen | 1.5 ml ml | EUR 37 |
Description: CryoProtX Mix Reagents 1.5 mL |
Set of 10 Biolipidure Reagents |
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Biolipidure-set | AS ONE International | 10mLx10 | EUR 1820.4 |
Description: Set of 10 Biolipidure Reagents, whose applications include Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
Custom production of antibodies in 5 Rats using customer supplied antigen (std 63 days protocol) |
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RAT-5 | Alpha Diagnostics | 1 | EUR 1365.6 |
Insect Cell Medium: Serum-Free Insect Culture Medium |
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ABP-MED-10002 | Allele Biotech | 1 liter | Ask for price |
Bovine serum albumin (BSA) protein (>99%, Protease-free) |
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BSA16-N-100 | Alpha Diagnostics | 100 mg | EUR 343.2 |
HSA Human Serum Albumin Recombinant Protein, Lipid Free |
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PROTP02768-3 | BosterBio | Regular: 50mg | EUR 565.2 |
Description: HSA Human Recombinant lipid reduced produced in Plant is a non-glycosylated, polypeptide chain containing 585 amino acids and having a molecular mass of 67 kDa. |
Exo-Flow 2.0 Basic Kit without antibody (Streptavidin beads + reagents) - for Serum or Plasma |
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EXOFLOW2-BASICA-SP | SBI | 30 rxn | EUR 1201.2 |
Rat Serum |
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abx098267-10ml | Abbexa | 10 ml | EUR 326.4 |
Bradford(Coomassie) Protein Assay Plus Reagents |
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P7201-050 | GenDepot | 450ml | EUR 225.6 |
CryoProtX Mix Eco Reagents 1.5 mL |
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M-MDSR-61-ECO | MiTeGen | 1.5 ml ml | EUR 37 |
Description: CryoProtX Mix Eco Reagents 1.5 mL |
Human Male AB Serum (Converted from Plasma), Xeno-Free |
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AR1005-0100 | Akron Biotech | 100mL | Ask for price |
Rabbit control serum (non-immunized, Disease free, SPF Rabbits) |
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NSPF-1 | Alpha Diagnostics | 1 ml | EUR 343.2 |
Rabbit control serum (non-immunized, Disease free, SPF Rabbits) |
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NSPF-10 | Alpha Diagnostics | 10 ml | EUR 781.2 |
Rabbit control serum (non-immunized, Disease free, SPF Rabbits) |
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NSPF-5 | Alpha Diagnostics | 5 ml | EUR 634.8 |
Bovine Serum Albumin ? Heat Shock, Protease Free, pH 7.0 |
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7919-100 | Biovision | each | EUR 783.6 |
Bovine Serum Albumin ? Heat Shock, Protease Free, pH 7.0 |
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7919-25 | Biovision | each | EUR 294 |