Isolation of Total RNA Using Monophasic Lysis Reagents

Isolation of Total RNA Using Monophasic Lysis Reagents

A typical mammalian cell contains ∼10^-5 µg of RNA, 80%-85% of which is ribosomal RNA (rRNA; chiefly the 28S, 18S, 5.8S, and 5S species). Most of the remaining 15%-20% consists of a variety of low-molecular-weight species (e.g., transfer RNAs [tRNAs] and small nuclear RNAs). These abundant RNAs are of defined size and sequence. In contrast, messenger RNA (mRNA), which makes up between 1% and 5% of the total cellular RNA, is heterogeneous in both size-from a few hundred bases to many kilobases in length-and sequence. In this introduction, the development and use of monophasic lysis reagents for the isolation of total RNA from eukaryotic cells is discussed.

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OOSA11794-100ML - SERUM:Azide free Serum & Cells
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PIG SERUM:Azide Free
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OOSA10413-5ML - Serum:Azide Free
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Albumin, Human Serum (Azide Free) (HSA)
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Albumin, Human Serum (Azide Free) (HSA)
MBS6120697-1mg	MyBiosource	1mg	EUR 1000

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MBS6120697-5x1mg	MyBiosource	5x1mg	EUR 4350

ALB (ALB, Serum albumin) (Azide free) (HRP)
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ALB (ALB, Serum albumin) (Azide free) (HRP)
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Albumin, Human Serum (BSA) (BSA & Azide Free)
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Albumin, Human Serum (BSA) (BSA & Azide Free)
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Human Serum Albumin Antigen, Reagent Grade
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Human Serum Albumin Antigen, Reagent Grade
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Albumin, Human Serum (BSA and Azide Free) (HSA)
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Albumin, Human Serum (BSA and Azide Free) (HSA)
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Bovine Serum Albumin ? Heat Shock, Reagent Grade, pH 7.0
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Bovine Serum Albumin ? Heat Shock, Reagent Grade, pH 7.0
7917-25	Biovision	each	EUR 294

Bovine Serum Albumin ? Heat Shock, Reagent Grade, pH 7.0
7917-5	Biovision	each	EUR 144

T-Pro Total Exosome Isolation reagent (from serum)
JO66-V002M	T-Pro Biotechnology	25ml/BT	EUR 800

T-Pro Total Exosome Isolation reagent (from serum)
JO66-V002S	T-Pro Biotechnology	1ml*5/set	EUR 200

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Albumin Depletion Reagent for Plasma and Serum (10 ml) NEW!
P5W13	101Bio	10 rxn	EUR 99

ExoQualiTM Overall Exosome Isolation Reagent (from serum)
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ExoQualiTM Overall Exosome Isolation Reagent (from serum)
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Recombinant Human Serum Albumin, From Plant (Reagent Grade)
H2630	Solarbio Life Science	Ask for price Ask for price Ask for price	500 mg 1 g 5 g

OPEF01351-100G - Human Serum Albumin Antigen Reagent Grade
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PON3, NT (Serum Paraoxonase/Lactonase 3) (Azide free) (HRP)
MBS6491998-02mL	MyBiosource	0.2mL	EUR 980

PON3, NT (Serum Paraoxonase/Lactonase 3) (Azide free) (HRP)
MBS6491998-5x02mL	MyBiosource	5x0.2mL	EUR 4250

MinuteTM Albumin Depletion Reagent for Plasma and Serum (20 ml)
WA-013	Inventbiotech	each	EUR 135

Human IgG (serum origin, purified >97%, low endotoxin, azide free)
20007-1-LE-1	Alpha Diagnostics	1 mg	EUR 196.8

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65186	Sisco Laboratories	10 Gms	EUR 38.4
Description: Part B

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MBS6344814-02mL	MyBiosource	0.2mL	EUR 980

SAA4, CT (SAA4, CSAA, Serum amyloid A-4 protein, Constitutively expressed serum amyloid A protein) (Azide free) (HRP)
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PON2, ID (PON2, Serum paraoxonase/arylesterase 2, Aromatic esterase 2, Serum aryldialkylphosphatase 2) (Azide free) (HRP)
MBS6335888-02mL	MyBiosource	0.2mL	EUR 980

PON2, ID (PON2, Serum paraoxonase/arylesterase 2, Aromatic esterase 2, Serum aryldialkylphosphatase 2) (Azide free) (HRP)
MBS6335888-5x02mL	MyBiosource	5x0.2mL	EUR 4250

PON2, NT (PON2, Serum paraoxonase/arylesterase 2, Aromatic esterase 2, Serum aryldialkylphosphatase 2) (Azide free) (HRP)
MBS6335899-02mL	MyBiosource	0.2mL	EUR 980

PON2, NT (PON2, Serum paraoxonase/arylesterase 2, Aromatic esterase 2, Serum aryldialkylphosphatase 2) (Azide free) (HRP)
MBS6335899-5x02mL	MyBiosource	5x0.2mL	EUR 4250

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SK8201	Bio Basic	100preps	EUR 112.2

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MBS318434-50mL	MyBiosource	50mL	EUR 1115

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MBS318434-5x50mL	MyBiosource	5x50mL	EUR 4765

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Reagents Module Assy.
U10071	DIALAB	1 Each	Ask for price

Human IgG-Biotin (serum origin, purified >97%, low endotoxin, azide free)
20007-1-LE-BTN	Alpha Diagnostics	100 ug	EUR 343.2

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MBS6274207-02mL	MyBiosource	0.2mL	EUR 980

APCS, CT (APCS, PTX2, Serum amyloid P-component, 9.5S alpha-1-glycoprotein, Serum amyloid P-component(1-203)) (Azide free) (HRP)
MBS6274207-5x02mL	MyBiosource	5x0.2mL	EUR 4250

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Myoglobin free serum
MBS663104-1Unit	MyBiosource	1Unit	EUR 845

Myoglobin free serum
MBS663104-5x1Unit	MyBiosource	5x1Unit	EUR 3460

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Cobalt-CatalyzedDefluorosilylation of Aryl Fluorides via Grignard Reagent Formation

Transition-metal-catalyzed transformations of the carbon-fluorine bond not only tackle an interesting problem of challenging bond activation but also offer new synthetic strategies where the relatively inert C-F bond is converted to versatile functional groups. Herein we report a practical cobalt-catalyzedsilylation of aryl fluorides that uses a cheap electrophilic silicon source with magnesium. This method is compatible with various silicon sources and can be operated under aerobic conditions. Mechanistic studies support the in situ formation of a Grignard reagent, which is captured by the electrophilic silicon source.

A DAPI-Based Reagent for Mass Cytometry

Multiparametric single cell analysis has seen dramatic improvements with the introduction of Mass Cytometry (MC) and Imaging Mass Cytometry (IMC™). These technologies expanded the number of biomarkers that can be identified simultaneously by using heavy isotope-tagged antibody reagents. Small molecule probes bearing heavy isotopes are emerging as additional useful functional reporters of cellular features.

Realizing this, we explored the iodination of DAPI to produce a heavy atom substituted derivative of the commonly used fluorescent DNA stain. Although exhibiting a drastically reduced fluorescence emission profile, I-DAPI retains strong binding affinity for DNA. I-DAPI was used to detect cellular DNA in MC and IMC™ assays with comparable efficiency to known Ir containing DNA intercalators. This work suggests repurposing of well-known colorimetric stains through simple reactions could be an effective strategy to develop new, functional MC and IMC™ reagents.

Cautionary note on contamination of reagents used for molecular detection of SARS-CoV-2

Reverse transcription (RT)-PCR, the principal diagnostic method applied in the world-wide struggle against COVID-19, is capable of detecting a single molecule of a viral genome. Correctly designed and practiced RT-PCR assays for SARS-CoV-2 should not cross react with similar but distinct viral pathogens, such as the coronaviruses associated with the common cold, and should perform with very high analytical sensitivity. This analytical performance is predicated on the ability of the method to detect the presence of the selected nucleic acid target, without detection of a false positive signal.

Influence of reagent lots and multiple measuring systems on estimating the coefficient of variation from quality control data; implications for uncertainty estimation and interpretation of QC results

• AbstractBackgroundClinical laboratories use internal quality control (QC) data to calculate standard deviation (SD) and coefficient of variation (CV) to estimate uncertainty of results and to interpret QC results. We examined the influence of different instruments, and QC and reagent lots on the CV calculated from QC data.MethodsResults for BioRadMultiqual frozen liquid QC samples over a 2-year interval were partitioned by QC and reagent lots.
• The mean and CV were calculated for each partition for each of three Abbott Architect c8000 instruments for measuring serum alanine amino transferase (ALT), creatinine (enzymatic), glucose and sodium.ResultsCVs differed among partitions and instruments for two QC levels by 5.8- and 3.3-fold for ALT, by 4.7- and 2.1-fold for creatinine, by 2.0- and 2.6-fold for glucose, and by 2.1- and 2.0-fold for sodium. Pooled CVs for two QC levels varied among instruments by 1.78- and 1.11-fold for ALT, by 1.63- and 1.11-fold for creatinine, by 1.08- and 1.06-fold for glucose, and by 1.24- and 1.31-fold for sodium.
• ConclusionsThe CVs from QC data varied substantially among QC and reagent lots and for different identical specification instruments. The CV used to estimate uncertainty for a measurement result or as the basis for interpreting individual QC results must be derived over a sufficient time interval to obtain a pooled CV that represents “typical” performance of a measuring system. An estimate of uncertainty provided to users of laboratory results will itself have uncertainty that can influence medical decisions.

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OOSA10411-5ML - SERUM:Azide Free Serum & Cells
OOSA10411-5ML	Aviva Systems Biology	5ml	EUR 99

OOSA10417-50ML - SERUM:Azide Free Serum & Cells
OOSA10417-50ML	Aviva Systems Biology	50ml	EUR 469

OOSA10426-20ML - SERUM:Azide Free Serum & Cells
OOSA10426-20ML	Aviva Systems Biology	20ml	EUR 99

OOSA10430-10ML - SERUM:Azide Free Serum & Cells
OOSA10430-10ML	Aviva Systems Biology	10ml	EUR 379

OOSA10419-100ML - SERUM:Azide Free Serum & Cells
OOSA10419-100ML	Aviva Systems Biology	100ml	EUR 749

OOSA11794-100ML - SERUM:Azide free Serum & Cells
OOSA11794-100ML	Aviva Systems Biology	100ml	EUR 1489

PIG SERUM:Azide Free
MBS239301-100mL	MyBiosource	100mL	EUR 220

PIG SERUM:Azide Free
MBS239301-5x100mL	MyBiosource	5x100mL	EUR 805

OOSA10413-5ML - Serum:Azide Free
OOSA10413-5ML	Aviva Systems Biology	5ml	EUR 69

OOSA10415-10ML - SERUM:Azide Free
OOSA10415-10ML	Aviva Systems Biology	10ml	EUR 139

OOSA10428-100ML - SERUM:Azide Free
OOSA10428-100ML	Aviva Systems Biology	100ml	EUR 139

Bovine Serum Albumin Fatty Acid Free Reagent Grade
GWB-F06E29	GenWay Biotech	1 kg	Ask for price

Bovine Serum Albumin Fatty Acid Protease Free Reagent Grade
GWB-DCC190	GenWay Biotech	1 kg	Ask for price

Free Chlorine Reagents 0-5mg/L - EACH
WAT1028	Scientific Laboratory Supplies	EACH	EUR 49.95

Albumin, Human Serum (Azide Free) (HSA)
MBS6120697-025mg	MyBiosource	0.25(mg	EUR 495

Albumin, Human Serum (Azide Free) (HSA)
MBS6120697-1mg	MyBiosource	1mg	EUR 1000

Albumin, Human Serum (Azide Free) (HSA)
MBS6120697-5x1mg	MyBiosource	5x1mg	EUR 4350

ALB (ALB, Serum albumin) (Azide free) (HRP)
MBS6121446-02mL	MyBiosource	0.2(mL	EUR 980

ALB (ALB, Serum albumin) (Azide free) (HRP)
MBS6121446-5x02mL	MyBiosource	5x0.2mL	EUR 4250

VEX Exosome Isolation Reagent (from serum)
R602	Vazyme	10 ml	EUR 769.2

Albumin, Human Serum (BSA) (BSA & Azide Free)
MBS6120698-025mg	MyBiosource	0.25(mg	EUR 445

Albumin, Human Serum (BSA) (BSA & Azide Free)
MBS6120698-1mg	MyBiosource	1mg	EUR 1015

Albumin, Human Serum (BSA) (BSA & Azide Free)
MBS6120698-5x1mg	MyBiosource	5x1mg	EUR 4415

Human Serum Albumin Antigen, Reagent Grade
MBS568775-100g	MyBiosource	100g	EUR 1240

Human Serum Albumin Antigen, Reagent Grade
MBS568775-5x100g	MyBiosource	5x100g	EUR 5385

Human Serum Albumin Antigen, Reagent Grade
ND-R0097	Novatein Biosciences, Inc.	1 Each	Ask for price

Albumin, Human Serum (BSA and Azide Free) (HSA)
MBS6120696-1mg	MyBiosource	1(mg	EUR 1055

Albumin, Human Serum (BSA and Azide Free) (HSA)
MBS6120696-5x1mg	MyBiosource	5x1mg	EUR 4600

Bovine Serum Albumin ? Heat Shock, Reagent Grade, pH 7.0
7917-100	Biovision	each	EUR 783.6

Bovine Serum Albumin ? Heat Shock, Reagent Grade, pH 7.0
7917-25	Biovision	each	EUR 294

Bovine Serum Albumin ? Heat Shock, Reagent Grade, pH 7.0
7917-5	Biovision	each	EUR 144

T-Pro Total Exosome Isolation reagent (from serum)
JO66-V002M	T-Pro Biotechnology	25ml/BT	EUR 800

T-Pro Total Exosome Isolation reagent (from serum)
JO66-V002S	T-Pro Biotechnology	1ml*5/set	EUR 200

Albumin Depletion Reagent for Plasma and Serum (10 ml) NEW!
P5W13	101Bio	10 rxn	EUR 99

ExoQualiTM Overall Exosome Isolation Reagent (from serum)
CEIK-044L-30T	Creative Bioarray	30 T	Ask for price

ExoQualiTM Overall Exosome Isolation Reagent (from serum)
CEIK-044L-50T	Creative Bioarray	50 T	Ask for price

Recombinant Human Serum Albumin, From Plant (Reagent Grade)
H2630	Solarbio Life Science	Ask for price Ask for price Ask for price	500 mg 1 g 5 g

OPEF01351-100G - Human Serum Albumin Antigen Reagent Grade
OPEF01351-100G	Aviva Systems Biology	100g	EUR 1302

PON3, NT (Serum Paraoxonase/Lactonase 3) (Azide free) (HRP)
MBS6491998-02mL	MyBiosource	0.2mL	EUR 980

PON3, NT (Serum Paraoxonase/Lactonase 3) (Azide free) (HRP)
MBS6491998-5x02mL	MyBiosource	5x0.2mL	EUR 4250

MinuteTM Albumin Depletion Reagent for Plasma and Serum (20 ml)
WA-013	Inventbiotech	each	EUR 135

RAT SERUM WITH 0.09% AZIDE Serum Products
GWB-Q00021	GenWay Biotech	10 ml	Ask for price

RAT SERUM WITH 0.09% AZIDE Serum Products
GWB-Q00022	GenWay Biotech	50 ml	Ask for price

RAT SERUM WITH 0.09% AZIDE Serum Products
GWB-Q00023	GenWay Biotech	100 ml	Ask for price

Human IgG (serum origin, purified >97%, low endotoxin, azide free)
20007-1-LE-1	Alpha Diagnostics	1 mg	EUR 196.8

Albumin Bovine (pH 7) fraction V Reagent Grade (Bovine Serum Albumin, BSA), 99%
65186	Sisco Laboratories	10 Gms	EUR 38.4
Description: Part B

SAA4, CT (SAA4, CSAA, Serum amyloid A-4 protein, Constitutively expressed serum amyloid A protein) (Azide free) (HRP)
MBS6344814-02mL	MyBiosource	0.2mL	EUR 980

SAA4, CT (SAA4, CSAA, Serum amyloid A-4 protein, Constitutively expressed serum amyloid A protein) (Azide free) (HRP)
MBS6344814-5x02mL	MyBiosource	5x0.2mL	EUR 4250

PON2, ID (PON2, Serum paraoxonase/arylesterase 2, Aromatic esterase 2, Serum aryldialkylphosphatase 2) (Azide free) (HRP)
MBS6335888-02mL	MyBiosource	0.2mL	EUR 980

PON2, ID (PON2, Serum paraoxonase/arylesterase 2, Aromatic esterase 2, Serum aryldialkylphosphatase 2) (Azide free) (HRP)
MBS6335888-5x02mL	MyBiosource	5x0.2mL	EUR 4250

PON2, NT (PON2, Serum paraoxonase/arylesterase 2, Aromatic esterase 2, Serum aryldialkylphosphatase 2) (Azide free) (HRP)
MBS6335899-02mL	MyBiosource	0.2mL	EUR 980

PON2, NT (PON2, Serum paraoxonase/arylesterase 2, Aromatic esterase 2, Serum aryldialkylphosphatase 2) (Azide free) (HRP)
MBS6335899-5x02mL	MyBiosource	5x0.2mL	EUR 4250

EZ-DNA Reagents
SK8201	Bio Basic	100preps	EUR 112.2

CRP Free Serum
GWB-FD777C	GenWay Biotech	50 ml	Ask for price

CRP Free Serum
MBS318434-50mL	MyBiosource	50mL	EUR 1115

CRP Free Serum
MBS318434-5x50mL	MyBiosource	5x50mL	EUR 4765

CRP free serum
MBS663102-1Unit	MyBiosource	1Unit	EUR 875

CRP free serum
MBS663102-5x1Unit	MyBiosource	5x1Unit	EUR 3530

Reagents Module Assy.
U10071	DIALAB	1 Each	Ask for price

Human IgG-Biotin (serum origin, purified >97%, low endotoxin, azide free)
20007-1-LE-BTN	Alpha Diagnostics	100 ug	EUR 343.2

APCS, CT (APCS, PTX2, Serum amyloid P-component, 9.5S alpha-1-glycoprotein, Serum amyloid P-component(1-203)) (Azide free) (HRP)
MBS6274207-02mL	MyBiosource	0.2mL	EUR 980

APCS, CT (APCS, PTX2, Serum amyloid P-component, 9.5S alpha-1-glycoprotein, Serum amyloid P-component(1-203)) (Azide free) (HRP)
MBS6274207-5x02mL	MyBiosource	5x0.2mL	EUR 4250

OOSA10429-10ML - Rat SERUM WITH 0.09% AZIDE Serum & Cells
OOSA10429-10ML	Aviva Systems Biology	10ml	EUR 279

OOSA10433-100ML - Rat SERUM WITH 0.09% AZIDE Serum & Cells
OOSA10433-100ML	Aviva Systems Biology	100ml	EUR 1799

Steroid Free Serum
90R-1007	Fitzgerald Industries International	50 mL	Ask for price

Ecl Detection Reagents
RPN3004	Scientific Laboratory Supplies	EACH	EUR 552.9

Myoglobin free serum
MBS663104-1Unit	MyBiosource	1Unit	EUR 845

Myoglobin free serum
MBS663104-5x1Unit	MyBiosource	5x1Unit	EUR 3460

RAT SERUM WITH 0.09% AZIDE
MBS238234-10mL	MyBiosource	10mL	EUR 385

RAT SERUM WITH 0.09% AZIDE
MBS238234-5x10mL	MyBiosource	5x10mL	EUR 1565

RAT SERUM WITH 0.09% AZIDE
MBS238236-100mL	MyBiosource	100mL	EUR 2170

RAT SERUM WITH 0.09% AZIDE
MBS238236-5x100mL	MyBiosource	5x100mL	EUR 9600

Rat EGF Construction Kit w/Developing Reagents
RRF312CKC	Antigenix America	-	Ask for price

Rat SCF Construction Kit w/Developing Reagents
RRF432CKC	Antigenix America	-	Ask for price

PON1, ID (PON1, PON, Serum paraoxonase/arylesterase 1, Aromatic esterase 1, K-45, Serum aryldialkylphosphatase 1) (Azide free) (HRP)
MBS6335877-02mL	MyBiosource	0.2mL	EUR 980

PON1, ID (PON1, PON, Serum paraoxonase/arylesterase 1, Aromatic esterase 1, K-45, Serum aryldialkylphosphatase 1) (Azide free) (HRP)
MBS6335877-5x02mL	MyBiosource	5x0.2mL	EUR 4250

Rat CNTF Construction Kit w/Developing Reagents
RRF426CKC	Antigenix America	-	Ask for price

Reagents Refrigiration Assy.
U10072	DIALAB	1 Each	Ask for price

Rat VEGF Construcition Kit w/Developing Reagents
RRF501CKC	Antigenix America	-	Ask for price

Rat RANTES Construction Kit w/Developing Reagents
RRF424CKC	Antigenix America	-	Ask for price

Rat Leptin Construction Kit w/Developing Reagents
RRF430CKC	Antigenix America	-	Ask for price

CHO Transfection Reagent and Protein Expression System I (Adherent Culture with Serum)
L3400	Solarbio Life Science	Ask for price Ask for price Ask for price	0.5 mL 1 mL 1.5 mL

Myeloperoxidase Free Serum
GWB-B28A30	GenWay Biotech	50 ml	Ask for price