Isolation of Total RNA Using Monophasic Lysis Reagents
A typical mammalian cell contains ∼10-5 µg of RNA, 80%-85% of which is ribosomal RNA (rRNA; chiefly the 28S, 18S, 5.8S, and 5S species). Most of the remaining 15%-20% consists of a variety of low-molecular-weight species (e.g., transfer RNAs [tRNAs] and small nuclear RNAs). These abundant RNAs are of defined size and sequence. In contrast, messenger RNA (mRNA), which makes up between 1% and 5% of the total cellular RNA, is heterogeneous in both size-from a few hundred bases to many kilobases in length-and sequence. In this introduction, the development and use of monophasic lysis reagents for the isolation of total RNA from eukaryotic cells is discussed.

kolgene
RABBIT SERUM:Azide Free Serum Products |
|||
| GWB-Q00020 | GenWay Biotech | 100 ml | Ask for price |
GUINEA PIG SERUM:Azide Free Serum Products |
|||
| GWB-Q00005 | GenWay Biotech | 5 ml | Ask for price |
OOSA10411-5ML - SERUM:Azide Free Serum & Cells |
|||
| OOSA10411-5ML | Aviva Systems Biology | 5ml | EUR 99 |
OOSA10417-50ML - SERUM:Azide Free Serum & Cells |
|||
| OOSA10417-50ML | Aviva Systems Biology | 50ml | EUR 469 |
OOSA10426-20ML - SERUM:Azide Free Serum & Cells |
|||
| OOSA10426-20ML | Aviva Systems Biology | 20ml | EUR 99 |
OOSA10430-10ML - SERUM:Azide Free Serum & Cells |
|||
| OOSA10430-10ML | Aviva Systems Biology | 10ml | EUR 379 |
OOSA10419-100ML - SERUM:Azide Free Serum & Cells |
|||
| OOSA10419-100ML | Aviva Systems Biology | 100ml | EUR 749 |
OOSA11794-100ML - SERUM:Azide free Serum & Cells |
|||
| OOSA11794-100ML | Aviva Systems Biology | 100ml | EUR 1489 |
PIG SERUM:Azide Free |
|||
| MBS239301-100mL | MyBiosource | 100mL | EUR 220 |
PIG SERUM:Azide Free |
|||
| MBS239301-5x100mL | MyBiosource | 5x100mL | EUR 805 |
OOSA10413-5ML - Serum:Azide Free |
|||
| OOSA10413-5ML | Aviva Systems Biology | 5ml | EUR 69 |
OOSA10415-10ML - SERUM:Azide Free |
|||
| OOSA10415-10ML | Aviva Systems Biology | 10ml | EUR 139 |
OOSA10428-100ML - SERUM:Azide Free |
|||
| OOSA10428-100ML | Aviva Systems Biology | 100ml | EUR 139 |
Bovine Serum Albumin Fatty Acid Free Reagent Grade |
|||
| GWB-F06E29 | GenWay Biotech | 1 kg | Ask for price |
Bovine Serum Albumin Fatty Acid Protease Free Reagent Grade |
|||
| GWB-DCC190 | GenWay Biotech | 1 kg | Ask for price |
Free Chlorine Reagents 0-5mg/L - EACH |
|||
| WAT1028 | Scientific Laboratory Supplies | EACH | EUR 49.95 |
Albumin, Human Serum (Azide Free) (HSA) |
|||
| MBS6120697-025mg | MyBiosource | 0.25(mg | EUR 495 |
Albumin, Human Serum (Azide Free) (HSA) |
|||
| MBS6120697-1mg | MyBiosource | 1mg | EUR 1000 |
Albumin, Human Serum (Azide Free) (HSA) |
|||
| MBS6120697-5x1mg | MyBiosource | 5x1mg | EUR 4350 |
VEX Exosome Isolation Reagent (from serum) |
|||
| R602 | Vazyme | 10 ml | EUR 769.2 |
Human Serum Albumin Antigen, Reagent Grade |
|||
| MBS568775-100g | MyBiosource | 100g | EUR 1240 |
Human Serum Albumin Antigen, Reagent Grade |
|||
| MBS568775-5x100g | MyBiosource | 5x100g | EUR 5385 |
ALB (ALB, Serum albumin) (Azide free) (HRP) |
|||
| MBS6121446-02mL | MyBiosource | 0.2(mL | EUR 980 |
ALB (ALB, Serum albumin) (Azide free) (HRP) |
|||
| MBS6121446-5x02mL | MyBiosource | 5x0.2mL | EUR 4250 |
EZ-DNA Reagents |
|||
| SK8201 | Bio Basic | 100preps | EUR 112.2 |
Albumin, Human Serum (BSA) (BSA & Azide Free) |
|||
| MBS6120698-025mg | MyBiosource | 0.25(mg | EUR 445 |
Albumin, Human Serum (BSA) (BSA & Azide Free) |
|||
| MBS6120698-1mg | MyBiosource | 1mg | EUR 1015 |
Albumin, Human Serum (BSA) (BSA & Azide Free) |
|||
| MBS6120698-5x1mg | MyBiosource | 5x1mg | EUR 4415 |
Bovine Serum Albumin ? Heat Shock, Reagent Grade, pH 7.0 |
|||
| 7917-100 | Biovision | each | EUR 783.6 |
Bovine Serum Albumin ? Heat Shock, Reagent Grade, pH 7.0 |
|||
| 7917-25 | Biovision | each | EUR 294 |
Bovine Serum Albumin ? Heat Shock, Reagent Grade, pH 7.0 |
|||
| 7917-5 | Biovision | each | EUR 144 |
T-Pro Total Exosome Isolation reagent (from serum) |
|||
| JO66-V002M | T-Pro Biotechnology | 25ml/BT | EUR 800 |
T-Pro Total Exosome Isolation reagent (from serum) |
|||
| JO66-V002S | T-Pro Biotechnology | 1ml*5/set | EUR 200 |
Ecl Detection Reagents |
|||
| RPN3004 | Scientific Laboratory Supplies | EACH | EUR 552.9 |
Albumin, Human Serum (BSA and Azide Free) (HSA) |
|||
| MBS6120696-1mg | MyBiosource | 1(mg | EUR 1055 |
Albumin, Human Serum (BSA and Azide Free) (HSA) |
|||
| MBS6120696-5x1mg | MyBiosource | 5x1mg | EUR 4600 |
Albumin Depletion Reagent for Plasma and Serum (10 ml) NEW! |
|||
| P5W13 | 101Bio | 10 rxn | EUR 99 |
MOUSE SERUM WITH 0.09% AZIDE Serum Products |
|||
| GWB-Q00007 | GenWay Biotech | 5 ml | Ask for price |
MOUSE SERUM WITH 0.09% AZIDE Serum Products |
|||
| GWB-Q00008 | GenWay Biotech | 10 ml | Ask for price |
MOUSE SERUM WITH 0.09% AZIDE Serum Products |
|||
| GWB-Q00009 | GenWay Biotech | 50 ml | Ask for price |
ExoQualiTM Overall Exosome Isolation Reagent (from serum) |
|||
| CEIK-044L-30T | Creative Bioarray | 30 T | Ask for price |
ExoQualiTM Overall Exosome Isolation Reagent (from serum) |
|||
| CEIK-044L-50T | Creative Bioarray | 50 T | Ask for price |
OPEF01351-100G - Human Serum Albumin Antigen Reagent Grade |
|||
| OPEF01351-100G | Aviva Systems Biology | 100g | EUR 1302 |
MinuteTM Albumin Depletion Reagent for Plasma and Serum (20 ml) |
|||
| WA-013 | Inventbiotech | each | EUR 135 |
Ecl Detection Reagents - EACH |
|||
| RPN2105 | Scientific Laboratory Supplies | EACH | EUR 815.4 |
CryoProtX Mix Reagents 1.5 mL |
|||
| M-MDSR-61 | MiTeGen | 1.5 ml ml | EUR 37 |
|
Description: CryoProtX Mix Reagents 1.5 mL |
|||
PON3, NT (Serum Paraoxonase/Lactonase 3) (Azide free) (HRP) |
|||
| MBS6491998-02mL | MyBiosource | 0.2mL | EUR 980 |
PON3, NT (Serum Paraoxonase/Lactonase 3) (Azide free) (HRP) |
|||
| MBS6491998-5x02mL | MyBiosource | 5x0.2mL | EUR 4250 |
Set of 10 Biolipidure Reagents |
|||
| Biolipidure-set | AS ONE International | 10mLx10 | EUR 1820.4 |
|
Description: Set of 10 Biolipidure Reagents, whose applications include Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
|||
REAGENTS HIGH RANGE AMMONIA - PK25 |
|||
| HI73325 | Scientific Laboratory Supplies | PK25 | EUR 55.35 |
High Range Ammonia Reagents - EACH |
|||
| HI9373301 | Scientific Laboratory Supplies | EACH | EUR 130.95 |
Albumin Bovine (pH 7) fraction V Reagent Grade (Bovine Serum Albumin, BSA), 99% |
|||
| 65186 | Sisco Laboratories | 10 Gms | EUR 38.4 |
|
Description: Part B |
|||
CryoProtX Mix Eco Reagents 1.5 mL |
|||
| M-MDSR-61-ECO | MiTeGen | 1.5 ml ml | EUR 37 |
|
Description: CryoProtX Mix Eco Reagents 1.5 mL |
|||
Human IgG (serum origin, purified >97%, low endotoxin, azide free) |
|||
| 20007-1-LE-1 | Alpha Diagnostics | 1 mg | EUR 196.8 |
PeliKine tool set, additional reagents |
|||
| M1980 | Tecan Benelux | 1 unit | EUR 169.8 |
MOUSE SERUM WITH 0.09% AZIDE |
|||
| MBS238221-5mL | MyBiosource | 5mL | EUR 190 |
MOUSE SERUM WITH 0.09% AZIDE |
|||
| MBS238221-5x5mL | MyBiosource | 5x5mL | EUR 680 |
MOUSE SERUM WITH 0.09% AZIDE |
|||
| MBS238222-10mL | MyBiosource | 10mL | EUR 250 |
MOUSE SERUM WITH 0.09% AZIDE |
|||
| MBS238222-5x10mL | MyBiosource | 5x10mL | EUR 960 |
Alkphos Direct Labelling Reagents - EACH |
|||
| RPN3680 | Scientific Laboratory Supplies | EACH | EUR 915.3 |
SAA4, CT (SAA4, CSAA, Serum amyloid A-4 protein, Constitutively expressed serum amyloid A protein) (Azide free) (HRP) |
|||
| MBS6344814-02mL | MyBiosource | 0.2mL | EUR 980 |
SAA4, CT (SAA4, CSAA, Serum amyloid A-4 protein, Constitutively expressed serum amyloid A protein) (Azide free) (HRP) |
|||
| MBS6344814-5x02mL | MyBiosource | 5x0.2mL | EUR 4250 |
PON2, ID (PON2, Serum paraoxonase/arylesterase 2, Aromatic esterase 2, Serum aryldialkylphosphatase 2) (Azide free) (HRP) |
|||
| MBS6335888-02mL | MyBiosource | 0.2mL | EUR 980 |
PON2, ID (PON2, Serum paraoxonase/arylesterase 2, Aromatic esterase 2, Serum aryldialkylphosphatase 2) (Azide free) (HRP) |
|||
| MBS6335888-5x02mL | MyBiosource | 5x0.2mL | EUR 4250 |
PON2, NT (PON2, Serum paraoxonase/arylesterase 2, Aromatic esterase 2, Serum aryldialkylphosphatase 2) (Azide free) (HRP) |
|||
| MBS6335899-02mL | MyBiosource | 0.2mL | EUR 980 |
PON2, NT (PON2, Serum paraoxonase/arylesterase 2, Aromatic esterase 2, Serum aryldialkylphosphatase 2) (Azide free) (HRP) |
|||
| MBS6335899-5x02mL | MyBiosource | 5x0.2mL | EUR 4250 |
CRP Free Serum |
|||
| GWB-FD777C | GenWay Biotech | 50 ml | Ask for price |
CRP Free Serum |
|||
| MBS318434-50mL | MyBiosource | 50mL | EUR 1115 |
CRP Free Serum |
|||
| MBS318434-5x50mL | MyBiosource | 5x50mL | EUR 4765 |
CRP free serum |
|||
| MBS663102-1Unit | MyBiosource | 1Unit | EUR 875 |
CRP free serum |
|||
| MBS663102-5x1Unit | MyBiosource | 5x1Unit | EUR 3530 |
Human IgG-Biotin (serum origin, purified >97%, low endotoxin, azide free) |
|||
| 20007-1-LE-BTN | Alpha Diagnostics | 100 ug | EUR 343.2 |
APCS, CT (APCS, PTX2, Serum amyloid P-component, 9.5S alpha-1-glycoprotein, Serum amyloid P-component(1-203)) (Azide free) (HRP) |
|||
| MBS6274207-02mL | MyBiosource | 0.2mL | EUR 980 |
APCS, CT (APCS, PTX2, Serum amyloid P-component, 9.5S alpha-1-glycoprotein, Serum amyloid P-component(1-203)) (Azide free) (HRP) |
|||
| MBS6274207-5x02mL | MyBiosource | 5x0.2mL | EUR 4250 |
Bradford(Coomassie) Protein Assay Plus Reagents |
|||
| P7201-050 | GenDepot | 450ml | EUR 118 |
Bradford(Coomassie) Protein Assay Plus Reagents |
|||
| P7201-100 | GenDepot | 950ml | EUR 130 |
iN-fect™ In vitro transfection reagents |
|||
| 15081 | iNtRON Biotechnology Inc | 500 µL | EUR 127.27 |
|
Description: Transfection |
|||
Pre-Titrated Reagents for Human EGF ELISA |
|||
| MBS590076-10Plates | MyBiosource | 10Plates | EUR 710 |
Pre-Titrated Reagents for Human EGF ELISA |
|||
| MBS590076-5Plates | MyBiosource | 5Plates | EUR 520 |
Pre-Titrated Reagents for Human EGF ELISA |
|||
| MBS590076-5x10Plates | MyBiosource | 5x10Plates | EUR 3270 |
Myoglobin free serum |
|||
| MBS663104-1Unit | MyBiosource | 1Unit | EUR 845 |
Myoglobin free serum |
|||
| MBS663104-5x1Unit | MyBiosource | 5x1Unit | EUR 3460 |
PON1, ID (PON1, PON, Serum paraoxonase/arylesterase 1, Aromatic esterase 1, K-45, Serum aryldialkylphosphatase 1) (Azide free) (HRP) |
|||
| MBS6335877-02mL | MyBiosource | 0.2mL | EUR 980 |
PON1, ID (PON1, PON, Serum paraoxonase/arylesterase 1, Aromatic esterase 1, K-45, Serum aryldialkylphosphatase 1) (Azide free) (HRP) |
|||
| MBS6335877-5x02mL | MyBiosource | 5x0.2mL | EUR 4250 |
Serum Free Medium For Mouse Neural Stem Cells |
|||
| MUXNF-90011 | Cyagen | 100mL | EUR 289 |
Liposomal and Non-Liposomal Transfection Reagents |
|||
| abx298032-100g | Abbexa | 100 µg | Ask for price |
Liposomal and Non-Liposomal Transfection Reagents |
|||
| abx298032-20g | Abbexa | 20 µg | EUR 875 |
Liposomal and Non-Liposomal Transfection Reagents |
|||
| abx298032-50g | Abbexa | 50 µg | Ask for price |
Pre-Titrated Reagents for Human IL-8 ELISA |
|||
| MBS590074-10Plates | MyBiosource | 10Plates | EUR 710 |
Pre-Titrated Reagents for Human IL-8 ELISA |
|||
| MBS590074-5Plates | MyBiosource | 5Plates | EUR 520 |
Pre-Titrated Reagents for Human IL-8 ELISA |
|||
| MBS590074-5x10Plates | MyBiosource | 5x10Plates | EUR 3270 |
Pre-Titrated Reagents for Human MCP-1 ELISA |
|||
| MBS590075-10Plates | MyBiosource | 10Plates | EUR 710 |
Pre-Titrated Reagents for Human MCP-1 ELISA |
|||
| MBS590075-5Plates | MyBiosource | 5Plates | EUR 520 |
Pre-Titrated Reagents for Human MCP-1 ELISA |
|||
| MBS590075-5x10Plates | MyBiosource | 5x10Plates | EUR 3270 |
Pre-Titrated Reagents for Human MCP-3 ELISA |
|||
| MBS590077-10Plates | MyBiosource | 10Plates | EUR 710 |
Pre-Titrated Reagents for Human MCP-3 ELISA |
|||
| MBS590077-5Plates | MyBiosource | 5Plates | EUR 520 |
Pre-Titrated Reagents for Human MCP-3 ELISA |
|||
| MBS590077-5x10Plates | MyBiosource | 5x10Plates | EUR 3270 |
Pre-Titrated Reagents for Human IL-16 ELISA |
|||
| MBS590080-10Plates | MyBiosource | 10Plates | EUR 710 |
Pre-Titrated Reagents for Human IL-16 ELISA |
|||
| MBS590080-5Plates | MyBiosource | 5Plates | EUR 520 |
Pre-Titrated Reagents for Human IL-16 ELISA |
|||
| MBS590080-5x10Plates | MyBiosource | 5x10Plates | EUR 3270 |
Pre-Titrated Reagents for Human IL-18 ELISA |
|||
| MBS590083-10Plates | MyBiosource | 10Plates | EUR 710 |
Pre-Titrated Reagents for Human IL-18 ELISA |
|||
| MBS590083-5Plates | MyBiosource | 5Plates | EUR 520 |
Pre-Titrated Reagents for Human IL-18 ELISA |
|||
| MBS590083-5x10Plates | MyBiosource | 5x10Plates | EUR 3270 |
Myeloperoxidase Free Serum |
|||
| GWB-B28A30 | GenWay Biotech | 50 ml | Ask for price |
Myeloperoxidase free serum |
|||
| MBS663103-1Unit | MyBiosource | 1Unit | EUR 845 |
Myeloperoxidase free serum |
|||
| MBS663103-5x1Unit | MyBiosource | 5x1Unit | EUR 3460 |
Pre-Titrated Reagents for Human GM-CSF ELISA |
|||
| MBS590079-10Plates | MyBiosource | 10Plates | EUR 710 |
Pre-Titrated Reagents for Human GM-CSF ELISA |
|||
| MBS590079-5Plates | MyBiosource | 5Plates | EUR 520 |
Pre-Titrated Reagents for Human GM-CSF ELISA |
|||
| MBS590079-5x10Plates | MyBiosource | 5x10Plates | EUR 3270 |
Troponin I Free Serum |
|||
| GWB-38F44D | GenWay Biotech | 50 ml | Ask for price |
Troponin I Free Serum |
|||
| MBS318430-50mL | MyBiosource | 50mL | EUR 1130 |
Troponin I Free Serum |
|||
| MBS318430-5x50mL | MyBiosource | 5x50mL | EUR 4830 |
Cystatin C free serum |
|||
| MBS663101-1Unit | MyBiosource | 1Unit | EUR 845 |
Cystatin C free serum |
|||
| MBS663101-5x1Unit | MyBiosource | 5x1Unit | EUR 3460 |
Pre-Titrated Reagents for Human TNF-alpha ELISA |
|||
| MBS590078-10Plates | MyBiosource | 10Plates | EUR 710 |
Pre-Titrated Reagents for Human TNF-alpha ELISA |
|||
| MBS590078-5Plates | MyBiosource | 5Plates | EUR 520 |
Pre-Titrated Reagents for Human TNF-alpha ELISA |
|||
| MBS590078-5x10Plates | MyBiosource | 5x10Plates | EUR 3270 |
Apo AI/B Free Serum |
|||
| GWB-9D7839 | GenWay Biotech | 100 ml | Ask for price |
Human Serum (FABP free) |
|||
| MBS539647-100mL | MyBiosource | 100mL | EUR 1615 |
Human Serum (FABP free) |
|||
| MBS539647-5x100mL | MyBiosource | 5x100mL | EUR 7035 |
Serum Free Medium (Type II) For Mouse Embryonic Stem Cells |
|||
| MUXES-90061 | Cyagen | 200mL | EUR 839 |
Serum Free Medium (Type I) For Mouse Embryonic Stem Cells |
|||
| MUXES-90062 | Cyagen | 200mL | EUR 839 |
RAT SERUM WITH 0.09% AZIDE Serum Products |
|||
| GWB-Q00021 | GenWay Biotech | 10 ml | Ask for price |
RAT SERUM WITH 0.09% AZIDE Serum Products |
|||
| GWB-Q00022 | GenWay Biotech | 50 ml | Ask for price |
RAT SERUM WITH 0.09% AZIDE Serum Products |
|||
| GWB-Q00023 | GenWay Biotech | 100 ml | Ask for price |
Human Serum (Myoglobin Free) |
|||
| MBS536546-100mL | MyBiosource | 100mL | EUR 1615 |
Human Serum (Myoglobin Free) |
|||
| MBS536546-5x100mL | MyBiosource | 5x100mL | EUR 7035 |
HEK 293 Media, Serum Free |
|||
| TBS8084-1L | Tribioscience | 1L | EUR 98 |
OOMA00062-50ML - CRP Free Serum |
|||
| OOMA00062-50ML | Aviva Systems Biology | 50ML | EUR 1379 |
RABBIT SERUM WITH 0.09% AZIDE Serum Products |
|||
| GWB-Q00017 | GenWay Biotech | 20 ml | Ask for price |
RABBIT SERUM WITH 0.09% AZIDE Serum Products |
|||
| GWB-Q00018 | GenWay Biotech | 100 ml | Ask for price |
MOUSE SERUM:Preservative Free |
|||
| MBS238201-5mL | MyBiosource | 5mL | EUR 220 |
MOUSE SERUM:Preservative Free |
|||
| MBS238201-5x5mL | MyBiosource | 5x5mL | EUR 750 |
MOUSE SERUM:Preservative Free |
|||
| MBS238202-10mL | MyBiosource | 10mL | EUR 250 |
MOUSE SERUM:Preservative Free |
|||
| MBS238202-5x10mL | MyBiosource | 5x10mL | EUR 950 |
MOUSE SERUM:Preservative Free |
|||
| MBS238203-50mL | MyBiosource | 50mL | EUR 625 |
MOUSE SERUM:Preservative Free |
|||
| MBS238203-5x50mL | MyBiosource | 5x50mL | EUR 2635 |
MOUSE SERUM:Preservative Free |
|||
| MBS238204-100mL | MyBiosource | 100mL | EUR 845 |
MOUSE SERUM:Preservative Free |
|||
| MBS238204-5x100mL | MyBiosource | 5x100mL | EUR 3620 |
GUINEA PIG SERUM WITH 0.09% AZIDE Serum Products |
|||
| GWB-Q00004 | GenWay Biotech | 5 ml | Ask for price |
Serum, Human, Troponin I Free |
|||
| MBS638864-10mL | MyBiosource | 10mL | EUR 505 |
Serum, Human, Troponin I Free |
|||
| MBS638864-50mL | MyBiosource | 50mL | EUR 1365 |
Serum, Human, Troponin I Free |
|||
| MBS638864-5x50mL | MyBiosource | 5x50mL | EUR 5985 |
SGK2, CT (Serine/Threonine-protein Kinase Sgk2, Serum/Glucocorticoid Regulated Kinase 2) (Azide free) (HRP) |
|||
| MBS6499136-02mL | MyBiosource | 0.2mL | EUR 980 |
SGK2, CT (Serine/Threonine-protein Kinase Sgk2, Serum/Glucocorticoid Regulated Kinase 2) (Azide free) (HRP) |
|||
| MBS6499136-5x02mL | MyBiosource | 5x0.2mL | EUR 4250 |
Cobalt-CatalyzedDefluorosilylation of Aryl Fluorides via Grignard Reagent Formation
Transition-metal-catalyzed transformations of the carbon-fluorine bond not only tackle an interesting problem of challenging bond activation but also offer new synthetic strategies where the relatively inert C-F bond is converted to versatile functional groups. Herein we report a practical cobalt-catalyzedsilylation of aryl fluorides that uses a cheap electrophilic silicon source with magnesium. This method is compatible with various silicon sources and can be operated under aerobic conditions. Mechanistic studies support the in situ formation of a Grignard reagent, which is captured by the electrophilic silicon source.
A DAPI-Based Reagent for Mass Cytometry
Multiparametric single cell analysis has seen dramatic improvements with the introduction of Mass Cytometry (MC) and Imaging Mass Cytometry (IMC™). These technologies expanded the number of biomarkers that can be identified simultaneously by using heavy isotope-tagged antibody reagents. Small molecule probes bearing heavy isotopes are emerging as additional useful functional reporters of cellular features.
Realizing this, we explored the iodination of DAPI to produce a heavy atom substituted derivative of the commonly used fluorescent DNA stain. Although exhibiting a drastically reduced fluorescence emission profile, I-DAPI retains strong binding affinity for DNA. I-DAPI was used to detect cellular DNA in MC and IMC™ assays with comparable efficiency to known Ir containing DNA intercalators. This work suggests repurposing of well-known colorimetric stains through simple reactions could be an effective strategy to develop new, functional MC and IMC™ reagents.
Cautionary note on contamination of reagents used for molecular detection of SARS-CoV-2
Reverse transcription (RT)-PCR, the principal diagnostic method applied in the world-wide struggle against COVID-19, is capable of detecting a single molecule of a viral genome. Correctly designed and practiced RT-PCR assays for SARS-CoV-2 should not cross react with similar but distinct viral pathogens, such as the coronaviruses associated with the common cold, and should perform with very high analytical sensitivity. This analytical performance is predicated on the ability of the method to detect the presence of the selected nucleic acid target, without detection of a false positive signal.
Influence of reagent lots and multiple measuring systems on estimating the coefficient of variation from quality control data; implications for uncertainty estimation and interpretation of QC results
- AbstractBackgroundClinical laboratories use internal quality control (QC) data to calculate standard deviation (SD) and coefficient of variation (CV) to estimate uncertainty of results and to interpret QC results. We examined the influence of different instruments, and QC and reagent lots on the CV calculated from QC data.MethodsResults for BioRadMultiqual frozen liquid QC samples over a 2-year interval were partitioned by QC and reagent lots.
- The mean and CV were calculated for each partition for each of three Abbott Architect c8000 instruments for measuring serum alanine amino transferase (ALT), creatinine (enzymatic), glucose and sodium.ResultsCVs differed among partitions and instruments for two QC levels by 5.8- and 3.3-fold for ALT, by 4.7- and 2.1-fold for creatinine, by 2.0- and 2.6-fold for glucose, and by 2.1- and 2.0-fold for sodium. Pooled CVs for two QC levels varied among instruments by 1.78- and 1.11-fold for ALT, by 1.63- and 1.11-fold for creatinine, by 1.08- and 1.06-fold for glucose, and by 1.24- and 1.31-fold for sodium.
- ConclusionsThe CVs from QC data varied substantially among QC and reagent lots and for different identical specification instruments. The CV used to estimate uncertainty for a measurement result or as the basis for interpreting individual QC results must be derived over a sufficient time interval to obtain a pooled CV that represents “typical” performance of a measuring system. An estimate of uncertainty provided to users of laboratory results will itself have uncertainty that can influence medical decisions.
MOUSE SERUM:Azide Free Serum Products |
|||
| GWB-Q00014 | GenWay Biotech | 500 ml | Ask for price |
RABBIT SERUM:Azide Free Serum Products |
|||
| GWB-Q00019 | GenWay Biotech | 20 ml | Ask for price |
RABBIT SERUM:Azide Free Serum Products |
|||
| GWB-Q00020 | GenWay Biotech | 100 ml | Ask for price |
GUINEA PIG SERUM:Azide Free Serum Products |
|||
| GWB-Q00005 | GenWay Biotech | 5 ml | Ask for price |
OOSA10411-5ML - SERUM:Azide Free Serum & Cells |
|||
| OOSA10411-5ML | Aviva Systems Biology | 5ml | EUR 99 |
OOSA10417-50ML - SERUM:Azide Free Serum & Cells |
|||
| OOSA10417-50ML | Aviva Systems Biology | 50ml | EUR 469 |
OOSA10426-20ML - SERUM:Azide Free Serum & Cells |
|||
| OOSA10426-20ML | Aviva Systems Biology | 20ml | EUR 99 |
OOSA10430-10ML - SERUM:Azide Free Serum & Cells |
|||
| OOSA10430-10ML | Aviva Systems Biology | 10ml | EUR 379 |
OOSA10419-100ML - SERUM:Azide Free Serum & Cells |
|||
| OOSA10419-100ML | Aviva Systems Biology | 100ml | EUR 749 |
OOSA11794-100ML - SERUM:Azide free Serum & Cells |
|||
| OOSA11794-100ML | Aviva Systems Biology | 100ml | EUR 1489 |
PIG SERUM:Azide Free |
|||
| MBS239301-100mL | MyBiosource | 100mL | EUR 220 |
PIG SERUM:Azide Free |
|||
| MBS239301-5x100mL | MyBiosource | 5x100mL | EUR 805 |
OOSA10413-5ML - Serum:Azide Free |
|||
| OOSA10413-5ML | Aviva Systems Biology | 5ml | EUR 69 |
OOSA10415-10ML - SERUM:Azide Free |
|||
| OOSA10415-10ML | Aviva Systems Biology | 10ml | EUR 139 |
OOSA10428-100ML - SERUM:Azide Free |
|||
| OOSA10428-100ML | Aviva Systems Biology | 100ml | EUR 139 |
Bovine Serum Albumin Fatty Acid Free Reagent Grade |
|||
| GWB-F06E29 | GenWay Biotech | 1 kg | Ask for price |
Bovine Serum Albumin Fatty Acid Protease Free Reagent Grade |
|||
| GWB-DCC190 | GenWay Biotech | 1 kg | Ask for price |
Free Chlorine Reagents 0-5mg/L - EACH |
|||
| WAT1028 | Scientific Laboratory Supplies | EACH | EUR 49.95 |
Albumin, Human Serum (Azide Free) (HSA) |
|||
| MBS6120697-025mg | MyBiosource | 0.25(mg | EUR 495 |
Albumin, Human Serum (Azide Free) (HSA) |
|||
| MBS6120697-1mg | MyBiosource | 1mg | EUR 1000 |
Albumin, Human Serum (Azide Free) (HSA) |
|||
| MBS6120697-5x1mg | MyBiosource | 5x1mg | EUR 4350 |
VEX Exosome Isolation Reagent (from serum) |
|||
| R602 | Vazyme | 10 ml | EUR 769.2 |
Human Serum Albumin Antigen, Reagent Grade |
|||
| MBS568775-100g | MyBiosource | 100g | EUR 1240 |
Human Serum Albumin Antigen, Reagent Grade |
|||
| MBS568775-5x100g | MyBiosource | 5x100g | EUR 5385 |
ALB (ALB, Serum albumin) (Azide free) (HRP) |
|||
| MBS6121446-02mL | MyBiosource | 0.2(mL | EUR 980 |
ALB (ALB, Serum albumin) (Azide free) (HRP) |
|||
| MBS6121446-5x02mL | MyBiosource | 5x0.2mL | EUR 4250 |
EZ-DNA Reagents |
|||
| SK8201 | Bio Basic | 100preps | EUR 112.2 |
Albumin, Human Serum (BSA) (BSA & Azide Free) |
|||
| MBS6120698-025mg | MyBiosource | 0.25(mg | EUR 445 |
Albumin, Human Serum (BSA) (BSA & Azide Free) |
|||
| MBS6120698-1mg | MyBiosource | 1mg | EUR 1015 |
Albumin, Human Serum (BSA) (BSA & Azide Free) |
|||
| MBS6120698-5x1mg | MyBiosource | 5x1mg | EUR 4415 |
Bovine Serum Albumin ? Heat Shock, Reagent Grade, pH 7.0 |
|||
| 7917-100 | Biovision | each | EUR 783.6 |
Bovine Serum Albumin ? Heat Shock, Reagent Grade, pH 7.0 |
|||
| 7917-25 | Biovision | each | EUR 294 |
Bovine Serum Albumin ? Heat Shock, Reagent Grade, pH 7.0 |
|||
| 7917-5 | Biovision | each | EUR 144 |
T-Pro Total Exosome Isolation reagent (from serum) |
|||
| JO66-V002M | T-Pro Biotechnology | 25ml/BT | EUR 800 |
T-Pro Total Exosome Isolation reagent (from serum) |
|||
| JO66-V002S | T-Pro Biotechnology | 1ml*5/set | EUR 200 |
Ecl Detection Reagents |
|||
| RPN3004 | Scientific Laboratory Supplies | EACH | EUR 552.9 |
Albumin, Human Serum (BSA and Azide Free) (HSA) |
|||
| MBS6120696-1mg | MyBiosource | 1(mg | EUR 1055 |
Albumin, Human Serum (BSA and Azide Free) (HSA) |
|||
| MBS6120696-5x1mg | MyBiosource | 5x1mg | EUR 4600 |
Albumin Depletion Reagent for Plasma and Serum (10 ml) NEW! |
|||
| P5W13 | 101Bio | 10 rxn | EUR 99 |
ExoQualiTM Overall Exosome Isolation Reagent (from serum) |
|||
| CEIK-044L-30T | Creative Bioarray | 30 T | Ask for price |
ExoQualiTM Overall Exosome Isolation Reagent (from serum) |
|||
| CEIK-044L-50T | Creative Bioarray | 50 T | Ask for price |
OPEF01351-100G - Human Serum Albumin Antigen Reagent Grade |
|||
| OPEF01351-100G | Aviva Systems Biology | 100g | EUR 1302 |
MinuteTM Albumin Depletion Reagent for Plasma and Serum (20 ml) |
|||
| WA-013 | Inventbiotech | each | EUR 135 |
Ecl Detection Reagents - EACH |
|||
| RPN2105 | Scientific Laboratory Supplies | EACH | EUR 815.4 |
CryoProtX Mix Reagents 1.5 mL |
|||
| M-MDSR-61 | MiTeGen | 1.5 ml ml | EUR 37 |
|
Description: CryoProtX Mix Reagents 1.5 mL |
|||
PON3, NT (Serum Paraoxonase/Lactonase 3) (Azide free) (HRP) |
|||
| MBS6491998-02mL | MyBiosource | 0.2mL | EUR 980 |
PON3, NT (Serum Paraoxonase/Lactonase 3) (Azide free) (HRP) |
|||
| MBS6491998-5x02mL | MyBiosource | 5x0.2mL | EUR 4250 |
Set of 10 Biolipidure Reagents |
|||
| Biolipidure-set | AS ONE International | 10mLx10 | EUR 1820.4 |
|
Description: Set of 10 Biolipidure Reagents, whose applications include Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
|||
REAGENTS HIGH RANGE AMMONIA - PK25 |
|||
| HI73325 | Scientific Laboratory Supplies | PK25 | EUR 55.35 |
High Range Ammonia Reagents - EACH |
|||
| HI9373301 | Scientific Laboratory Supplies | EACH | EUR 130.95 |
Albumin Bovine (pH 7) fraction V Reagent Grade (Bovine Serum Albumin, BSA), 99% |
|||
| 65186 | Sisco Laboratories | 10 Gms | EUR 38.4 |
|
Description: Part B |
|||
RAT SERUM WITH 0.09% AZIDE Serum Products |
|||
| GWB-Q00021 | GenWay Biotech | 10 ml | Ask for price |
RAT SERUM WITH 0.09% AZIDE Serum Products |
|||
| GWB-Q00022 | GenWay Biotech | 50 ml | Ask for price |
RAT SERUM WITH 0.09% AZIDE Serum Products |
|||
| GWB-Q00023 | GenWay Biotech | 100 ml | Ask for price |
CryoProtX Mix Eco Reagents 1.5 mL |
|||
| M-MDSR-61-ECO | MiTeGen | 1.5 ml ml | EUR 37 |
|
Description: CryoProtX Mix Eco Reagents 1.5 mL |
|||
Human IgG (serum origin, purified >97%, low endotoxin, azide free) |
|||
| 20007-1-LE-1 | Alpha Diagnostics | 1 mg | EUR 196.8 |
PeliKine tool set, additional reagents |
|||
| M1980 | Tecan Benelux | 1 unit | EUR 169.8 |
Alkphos Direct Labelling Reagents - EACH |
|||
| RPN3680 | Scientific Laboratory Supplies | EACH | EUR 915.3 |
SAA4, CT (SAA4, CSAA, Serum amyloid A-4 protein, Constitutively expressed serum amyloid A protein) (Azide free) (HRP) |
|||
| MBS6344814-02mL | MyBiosource | 0.2mL | EUR 980 |
SAA4, CT (SAA4, CSAA, Serum amyloid A-4 protein, Constitutively expressed serum amyloid A protein) (Azide free) (HRP) |
|||
| MBS6344814-5x02mL | MyBiosource | 5x0.2mL | EUR 4250 |
PON2, ID (PON2, Serum paraoxonase/arylesterase 2, Aromatic esterase 2, Serum aryldialkylphosphatase 2) (Azide free) (HRP) |
|||
| MBS6335888-02mL | MyBiosource | 0.2mL | EUR 980 |
PON2, ID (PON2, Serum paraoxonase/arylesterase 2, Aromatic esterase 2, Serum aryldialkylphosphatase 2) (Azide free) (HRP) |
|||
| MBS6335888-5x02mL | MyBiosource | 5x0.2mL | EUR 4250 |
PON2, NT (PON2, Serum paraoxonase/arylesterase 2, Aromatic esterase 2, Serum aryldialkylphosphatase 2) (Azide free) (HRP) |
|||
| MBS6335899-02mL | MyBiosource | 0.2mL | EUR 980 |
PON2, NT (PON2, Serum paraoxonase/arylesterase 2, Aromatic esterase 2, Serum aryldialkylphosphatase 2) (Azide free) (HRP) |
|||
| MBS6335899-5x02mL | MyBiosource | 5x0.2mL | EUR 4250 |
CRP Free Serum |
|||
| GWB-FD777C | GenWay Biotech | 50 ml | Ask for price |
CRP Free Serum |
|||
| MBS318434-50mL | MyBiosource | 50mL | EUR 1115 |
CRP Free Serum |
|||
| MBS318434-5x50mL | MyBiosource | 5x50mL | EUR 4765 |
CRP free serum |
|||
| MBS663102-1Unit | MyBiosource | 1Unit | EUR 875 |
CRP free serum |
|||
| MBS663102-5x1Unit | MyBiosource | 5x1Unit | EUR 3530 |
Human IgG-Biotin (serum origin, purified >97%, low endotoxin, azide free) |
|||
| 20007-1-LE-BTN | Alpha Diagnostics | 100 ug | EUR 343.2 |
APCS, CT (APCS, PTX2, Serum amyloid P-component, 9.5S alpha-1-glycoprotein, Serum amyloid P-component(1-203)) (Azide free) (HRP) |
|||
| MBS6274207-02mL | MyBiosource | 0.2mL | EUR 980 |
APCS, CT (APCS, PTX2, Serum amyloid P-component, 9.5S alpha-1-glycoprotein, Serum amyloid P-component(1-203)) (Azide free) (HRP) |
|||
| MBS6274207-5x02mL | MyBiosource | 5x0.2mL | EUR 4250 |
OOSA10429-10ML - Rat SERUM WITH 0.09% AZIDE Serum & Cells |
|||
| OOSA10429-10ML | Aviva Systems Biology | 10ml | EUR 279 |
OOSA10433-100ML - Rat SERUM WITH 0.09% AZIDE Serum & Cells |
|||
| OOSA10433-100ML | Aviva Systems Biology | 100ml | EUR 1799 |
Bradford(Coomassie) Protein Assay Plus Reagents |
|||
| P7201-050 | GenDepot | 450ml | EUR 118 |
Bradford(Coomassie) Protein Assay Plus Reagents |
|||
| P7201-100 | GenDepot | 950ml | EUR 130 |
iN-fect™ In vitro transfection reagents |
|||
| 15081 | iNtRON Biotechnology Inc | 500 µL | EUR 127.27 |
|
Description: Transfection |
|||
Pre-Titrated Reagents for Human EGF ELISA |
|||
| MBS590076-10Plates | MyBiosource | 10Plates | EUR 710 |
Pre-Titrated Reagents for Human EGF ELISA |
|||
| MBS590076-5Plates | MyBiosource | 5Plates | EUR 520 |
Pre-Titrated Reagents for Human EGF ELISA |
|||
| MBS590076-5x10Plates | MyBiosource | 5x10Plates | EUR 3270 |
Myoglobin free serum |
|||
| MBS663104-1Unit | MyBiosource | 1Unit | EUR 845 |
Myoglobin free serum |
|||
| MBS663104-5x1Unit | MyBiosource | 5x1Unit | EUR 3460 |
PON1, ID (PON1, PON, Serum paraoxonase/arylesterase 1, Aromatic esterase 1, K-45, Serum aryldialkylphosphatase 1) (Azide free) (HRP) |
|||
| MBS6335877-02mL | MyBiosource | 0.2mL | EUR 980 |
PON1, ID (PON1, PON, Serum paraoxonase/arylesterase 1, Aromatic esterase 1, K-45, Serum aryldialkylphosphatase 1) (Azide free) (HRP) |
|||
| MBS6335877-5x02mL | MyBiosource | 5x0.2mL | EUR 4250 |
RAT SERUM WITH 0.09% AZIDE |
|||
| MBS238234-10mL | MyBiosource | 10mL | EUR 385 |
RAT SERUM WITH 0.09% AZIDE |
|||
| MBS238234-5x10mL | MyBiosource | 5x10mL | EUR 1565 |
RAT SERUM WITH 0.09% AZIDE |
|||
| MBS238236-100mL | MyBiosource | 100mL | EUR 2170 |
