Isolation of Total RNA Using Monophasic Lysis Reagents

Isolation of Total RNA Using Monophasic Lysis Reagents

A typical mammalian cell contains ∼10^-5 µg of RNA, 80%-85% of which is ribosomal RNA (rRNA; chiefly the 28S, 18S, 5.8S, and 5S species). Most of the remaining 15%-20% consists of a variety of low-molecular-weight species (e.g., transfer RNAs [tRNAs] and small nuclear RNAs). These abundant RNAs are of defined size and sequence. In contrast, messenger RNA (mRNA), which makes up between 1% and 5% of the total cellular RNA, is heterogeneous in both size-from a few hundred bases to many kilobases in length-and sequence. In this introduction, the development and use of monophasic lysis reagents for the isolation of total RNA from eukaryotic cells is discussed.

Serum Albumin, Lipid Free Protein
20-abx260049	Abbexa	EUR 927.60 EUR 343.20 EUR 627.60	100 mg 10 mg 50 mg

Bovine Serum Albumin, protease free
GK4012-1KG	Glentham Life Sciences	1 kg	EUR 1165.2

Bovine Serum Albumin, protease free
GK4012-500G	Glentham Life Sciences	500 g	EUR 638.4

PK15 Cell Serum-Free Medium
VCum-Lsx0007	Creative Biolabs	1 L	EUR 909.6
Description: PK15 cell serum-free medium has been specifically developed for suspension culture of PK-15 cells.

CHO Cell Serum-Free Medium
VCum-Lsx0025	Creative Biolabs	1L	EUR 762
Description: A serum-free medium that is ideal for suspension culture of CHO cell lines (CHO DG44, CHO-K1 and CHO-S).

HEK Cell Serum-Free Medium
VCum-Lsx0027	Creative Biolabs	1L	EUR 762
Description: A serum-free medium that is ideal for suspension culture of HEK cell lines.

MDBK Cell Serum-Free Medium
VCum-Lsx0029	Creative Biolabs	1L	EUR 818.4
Description: Used in serum-free suspension MDBK cell line culture for bovine virus diarrhea or bovine rhinotracheitis vaccine production.

Vero Cell Serum-Free Medium
VCum-Lsx0031	Creative Biolabs	1 L	EUR 909.6
Description: A serum-free medium that is ideal for suspension culture of Vero cell lines.

500mL Insectagro DS2 Serum-Free
13-402-CV	Scientific Laboratory Supplies	PK6	EUR 222.3

500mL INSECTAGRO SF9 serum free
13-410-CV	Scientific Laboratory Supplies	PK6	EUR 192.66

EZ-DNA Reagents
BS8202	Bio Basic	100preps	EUR 105.67

EZ-DNA Reagents
SK8201	Bio Basic	100preps	EUR 112.2

Ecl Detection Reagents
RPN2105	Scientific Laboratory Supplies	EACH	EUR 611.04

Ecl Detection Reagents
RPN3004	Scientific Laboratory Supplies	EACH	EUR 489.06

Fetal Bovine Serum, Premium Tetracyclin Free
F0500-050	GenDepot	500ml	EUR 1388.4

B-27 Serum-Free Supplement (50x)
abx082466-10ml	Abbexa	10 ml	EUR 393.6

Normal Bovine Serum (Protease/IgG free)
88R-1012	Fitzgerald	10 ml	EUR 218.4
Description: Normal Bovine Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Bovine Serum Albumin, fatty acid free
GX5685-1KG	Glentham Life Sciences	1 kg	EUR 1442.4

Bovine Serum Albumin, fatty acid free
GX5685-500G	Glentham Life Sciences	500 g	EUR 792

ST Cell Serum-Free Medium-1
VCum-Lsx0001	Creative Biolabs	1 L	EUR 888
Description: ST cell serum-free medium has been specifically developed for suspension culture of ST cells.

ST Cell Serum-Free Medium-2
VCum-Lsx0003	Creative Biolabs	1 L	EUR 909.6
Description: ST cell serum-free medium has been specifically developed for suspension culture of ST cells.

PK15 Cell Serum-Free Medium (Powder)
VCum-Lsx0008-10L	Creative Biolabs	10 L	EUR 2798.4
Description: PK15 cell serum-free medium has been specifically developed for suspension culture of PK-15 cells.

PK15 Cell Serum-Free Medium (Powder)
VCum-Lsx0008-1L	Creative Biolabs	1 L	EUR 333.6
Description: PK15 cell serum-free medium has been specifically developed for suspension culture of PK-15 cells.

PK15 Cell Serum-Free Medium (Powder)
VCum-Lsx0008-5L	Creative Biolabs	5 L	EUR 1429.2
Description: PK15 cell serum-free medium has been specifically developed for suspension culture of PK-15 cells.

MDCK Cell Serum-Free Medium-1
VCum-Lsx0009	Creative Biolabs	1 L	EUR 818.4
Description: MDCK cell serum-free medium has been specifically developed for suspension culture of MDCK cells.

MDCK Cell Serum-Free Medium-2
VCum-Lsx0011	Creative Biolabs	1 L	EUR 832.8
Description: Used in serum-free suspension MDCK cell line culture for avian influenza vaccine production.

BHK Cell Serum-Free Medium-1
VCum-Lsx0017	Creative Biolabs	1 L	EUR 776.4
Description: BHK cell serum-free medium has been specifically developed for suspension culture of BHK cells.

BHK Cell Serum-Free Medium-2
VCum-Lsx0019	Creative Biolabs	1 L	EUR 860.4
Description: Used in serum-free suspension BHK cell line culture for newcastle disease virus production.

Insect Cell Serum-Free Medium-1
VCum-Lsx0021	Creative Biolabs	1 L	EUR 888
Description: A serum-free medium that is ideal for suspension culture of insect cell lines (HighFive, SF9).

Insect Cell Serum-Free Medium-2
VCum-Lsx0023	Creative Biolabs	1 L	EUR 909.6
Description: A serum-free medium that is ideal for suspension culture of insect cell lines (HighFive, SF9).

CHO Cell Serum-Free Medium (Powder)
VCum-Lsx0026-10L	Creative Biolabs	10 L	EUR 2096.4
Description: A serum-free medium that is ideal for suspension culture of CHO cell lines (CHO DG44, CHO-K1 and CHO-S).

CHO Cell Serum-Free Medium (Powder)
VCum-Lsx0026-1L	Creative Biolabs	1 L	EUR 264
Description: A serum-free medium that is ideal for suspension culture of CHO cell lines (CHO DG44, CHO-K1 and CHO-S).

CHO Cell Serum-Free Medium (Powder)
VCum-Lsx0026-5L	Creative Biolabs	5 L	EUR 1077.6
Description: A serum-free medium that is ideal for suspension culture of CHO cell lines (CHO DG44, CHO-K1 and CHO-S).

HEK Cell Serum-Free Medium (Powder)
VCum-Lsx0028	Creative Biolabs	50 L	EUR 7693.2
Description: A serum-free medium that is ideal for suspension culture of HEK cell lines.

MDBK Cell Serum-Free Medium (Powder)
VCum-Lsx0030	Creative Biolabs	50 L	EUR 10309.2
Description: Used in serum-free suspension MDBK cell line culture for bovine virus diarrhea or bovine rhinotracheitis vaccine production.

Vero Cell Serum-Free Medium (Powder)
VCum-Lsx0032-10L	Creative Biolabs	10 L	EUR 2798.4
Description: A serum-free medium that is ideal for suspension culture of Vero cell lines.

Vero Cell Serum-Free Medium (Powder)
VCum-Lsx0032-1L	Creative Biolabs	1 L	EUR 338.4
Description: A serum-free medium that is ideal for suspension culture of Vero cell lines.

Vero Cell Serum-Free Medium (Powder)
VCum-Lsx0032-5L	Creative Biolabs	5 L	EUR 1449.6
Description: A serum-free medium that is ideal for suspension culture of Vero cell lines.

Corning Lymphocyte Serum Free Medium KBM551
88-551-CM	Scientific Laboratory Supplies	1L	EUR 182.4

Corning Lymphocyte Serum Free Medium KBM581
88-581-CM	Scientific Laboratory Supplies	1L	EUR 240.54

REAGENTS HIGH RANGE AMMONIA
HI73325	Scientific Laboratory Supplies	PK25	EUR 38.19

High Range Ammonia Reagents
HI9373301	Scientific Laboratory Supplies	EACH	EUR 91.2

Alkphos Direct Labelling Reagents
RPN3680	Scientific Laboratory Supplies	EACH	EUR 686.28

Human Apolipoprotein AI / B Free Serum Protein
abx060869-100ml	Abbexa	100 ml	EUR 594

ST Cell Serum-Free Medium-1 (Powder)
VCum-Lsx0002-10L	Creative Biolabs	10 L	EUR 2658
Description: ST cell serum-free medium has been specifically developed for suspension culture of ST cells.

ST Cell Serum-Free Medium-1 (Powder)
VCum-Lsx0002-1L	Creative Biolabs	1 L	EUR 319.2
Description: ST cell serum-free medium has been specifically developed for suspension culture of ST cells.

ST Cell Serum-Free Medium-1 (Powder)
VCum-Lsx0002-5L	Creative Biolabs	5 L	EUR 1358.4
Description: ST cell serum-free medium has been specifically developed for suspension culture of ST cells.

ST Cell Serum-Free Medium-2 (Powder)
VCum-Lsx0004-10L	Creative Biolabs	10 L	EUR 2798.4
Description: ST cell serum-free medium has been specifically developed for suspension culture of ST cells.

ST Cell Serum-Free Medium-2 (Powder)
VCum-Lsx0004-1L	Creative Biolabs	1 L	EUR 333.6
Description: ST cell serum-free medium has been specifically developed for suspension culture of ST cells.

ST Cell Serum-Free Medium-2 (Powder)
VCum-Lsx0004-5L	Creative Biolabs	5 L	EUR 1429.2
Description: ST cell serum-free medium has been specifically developed for suspension culture of ST cells.

MDCK Cell Serum-Free Medium-1 (Powder)
VCum-Lsx0010-10L	Creative Biolabs	10 L	EUR 2236.8
Description: MDCK cell serum-free medium has been specifically developed for suspension culture of MDCK cells.

MDCK Cell Serum-Free Medium-1 (Powder)
VCum-Lsx0010-1L	Creative Biolabs	1 L	EUR 277.2
Description: MDCK cell serum-free medium has been specifically developed for suspension culture of MDCK cells.

MDCK Cell Serum-Free Medium-1 (Powder)
VCum-Lsx0010-5L	Creative Biolabs	5 L	EUR 1148.4
Description: MDCK cell serum-free medium has been specifically developed for suspension culture of MDCK cells.

MDCK Cell Serum-Free Medium-2 (Powder)
VCum-Lsx0012-10L	Creative Biolabs	10 L	EUR 2306.4
Description: Used in serum-free suspension MDCK cell line culture for avian influenza vaccine production.

MDCK Cell Serum-Free Medium-2 (Powder)
VCum-Lsx0012-1L	Creative Biolabs	1 L	EUR 284.4
Description: Used in serum-free suspension MDCK cell line culture for avian influenza vaccine production.

MDCK Cell Serum-Free Medium-2 (Powder)
VCum-Lsx0012-5L	Creative Biolabs	5 L	EUR 1183.2
Description: Used in serum-free suspension MDCK cell line culture for avian influenza vaccine production.

BHK Cell Serum-Free Medium-1 (Powder)
VCum-Lsx0018-10L	Creative Biolabs	10 L	EUR 2025.6
Description: BHK cellserum-free medium has been specifically developed for suspension culture of BHK cells.

BHK Cell Serum-Free Medium-1 (Powder)
VCum-Lsx0018-1L	Creative Biolabs	1 L	EUR 256.8
Description: BHK cellserum-free medium has been specifically developed for suspension culture of BHK cells.

BHK Cell Serum-Free Medium-1 (Powder)
VCum-Lsx0018-5L	Creative Biolabs	5 L	EUR 1042.8
Description: BHK cellserum-free medium has been specifically developed for suspension culture of BHK cells.

BHK Cell Serum-Free Medium-2 (Powder)
VCum-Lsx0020-10L	Creative Biolabs	10 L	EUR 2306.4
Description: Used in serum-free suspension BHK cell line culture for newcastle disease virus production.

BHK Cell Serum-Free Medium-2 (Powder)
VCum-Lsx0020-1L	Creative Biolabs	1 L	EUR 284.4
Description: Used in serum-free suspension BHK cell line culture for newcastle disease virus production.

BHK Cell Serum-Free Medium-2 (Powder)
VCum-Lsx0020-5L	Creative Biolabs	5 L	EUR 1183.2
Description: Used in serum-free suspension BHK cell line culture for newcastle disease virus production.

Insect Cell Serum-Free Medium-1 (Powder)
VCum-Lsx0022-10L	Creative Biolabs	10 L	EUR 2658
Description: A serum-free medium that is ideal for suspension culture of insect cell lines (HighFive, SF9).

Insect Cell Serum-Free Medium-1 (Powder)
VCum-Lsx0022-1L	Creative Biolabs	1 L	EUR 319.2
Description: A serum-free medium that is ideal for suspension culture of insect cell lines (HighFive, SF9).

Insect Cell Serum-Free Medium-1 (Powder)
VCum-Lsx0022-5L	Creative Biolabs	5 L	EUR 1358.4
Description: A serum-free medium that is ideal for suspension culture of insect cell lines (HighFive, SF9).

Insect Cell Serum-Free Medium-2 (Powder)
VCum-Lsx0024-10L	Creative Biolabs	10 L	EUR 2798.4
Description: A serum-free medium that is ideal for suspension culture of insect cell lines (HighFive, SF9).

Insect Cell Serum-Free Medium-2 (Powder)
VCum-Lsx0024-1L	Creative Biolabs	1 L	EUR 333.6
Description: A serum-free medium that is ideal for suspension culture of insect cell lines (HighFive, SF9).

Insect Cell Serum-Free Medium-2 (Powder)
VCum-Lsx0024-5L	Creative Biolabs	5 L	EUR 1429.2
Description: A serum-free medium that is ideal for suspension culture of insect cell lines (HighFive, SF9).

CryoProtX Mix Reagents 1.5 mL
M-MDSR-61	MiTeGen	1.5 ml ml	EUR 37
Description: CryoProtX Mix Reagents 1.5 mL

Set of 10 Biolipidure Reagents
Biolipidure-set	AS ONE International	10mLx10	EUR 1820.4
Description: Set of 10 Biolipidure Reagents, whose applications include Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement

Bovine serum albumin (BSA) protein (>99%, Protease-free)
BSA16-N-100	Alpha Diagnostics	100 mg	EUR 343.2

Insect Cell Medium: Serum-Free Insect Culture Medium
ABP-MED-10002	Allele Biotech	1 liter	Ask for price

HSA Human Serum Albumin Recombinant Protein, Lipid Free
PROTP02768-3	BosterBio	Regular: 50mg	EUR 565.2
Description: HSA Human Recombinant lipid reduced produced in Plant is a non-glycosylated, polypeptide chain containing 585 amino acids and having a molecular mass of 67 kDa.

Exo-Flow 2.0 Basic Kit without antibody (Streptavidin beads + reagents) - for Serum or Plasma
EXOFLOW2-BASICA-SP	SBI	30 rxn	EUR 1201.2

Mouse Serum
abx098261-100L	Abbexa	100 L	EUR 155407.2

Mouse Serum
20-abx098261	Abbexa	EUR 1395.60 EUR 326.40	100 ml 10 ml

Mouse Serum
abx098261-5L	Abbexa	5 L	EUR 10599.6

Mouse Serum
abx098262-1ml	Abbexa	1 ml	EUR 2064

Bradford(Coomassie) Protein Assay Plus Reagents
P7201-050	GenDepot	450ml	EUR 225.6

CryoProtX Mix Eco Reagents 1.5 mL
M-MDSR-61-ECO	MiTeGen	1.5 ml ml	EUR 37
Description: CryoProtX Mix Eco Reagents 1.5 mL

Purified rat serum albumin protein (RSA, >99%, globulin free)
ALBR13-N-10	Alpha Diagnostics	10 mg	EUR 343.2

Human Male AB Serum (Converted from Plasma), Xeno-Free
AR1005-0100	Akron Biotech	100mL	Ask for price

Rabbit control serum (non-immunized, Disease free, SPF Rabbits)
NSPF-1	Alpha Diagnostics	1 ml	EUR 343.2

Rabbit control serum (non-immunized, Disease free, SPF Rabbits)
NSPF-10	Alpha Diagnostics	10 ml	EUR 781.2

Rabbit control serum (non-immunized, Disease free, SPF Rabbits)
NSPF-5	Alpha Diagnostics	5 ml	EUR 634.8

Bovine Serum Albumin ? Heat Shock, Protease Free, pH 7.0
7919-100	Biovision	each	EUR 783.6

Bovine Serum Albumin ? Heat Shock, Protease Free, pH 7.0
7919-25	Biovision	each	EUR 294

Bovine Serum Albumin ? Heat Shock, Protease Free, pH 7.0
7919-5	Biovision	each	EUR 144

Human IgG (serum origin, purified >97%, low endotoxin, azide free)
20007-1-LE-1	Alpha Diagnostics	1 mg	EUR 196.8

Bovine Serum Albumin ? Heat Shock, Protease DNASE Free, pH 7.0
7920-100	Biovision	each	EUR 1096.8

Bovine Serum Albumin ? Heat Shock, Protease DNASE Free, pH 7.0
7920-1000	Biovision	each	Ask for price

Bovine Serum Albumin ? Heat Shock, Protease DNASE Free, pH 7.0
7920-25	Biovision	each	EUR 444

Bovine Serum Albumin ? Heat Shock, Protease DNASE Free, pH 7.0
7920-5	Biovision	each	EUR 183.6

Bovine Serum Albumin ? Heat Shock, Fatty Acid Free, pH 7.0
7921-100	Biovision	each	EUR 1358.4

Bovine Serum Albumin ? Heat Shock, Fatty Acid Free, pH 7.0
7921-25	Biovision	each	EUR 535.2

Bovine Serum Albumin ? Heat Shock, Fatty Acid Free, pH 7.0
7921-5	Biovision	each	EUR 210

Mouse Serum Albumin
30R-3304	Fitzgerald	10 mg	EUR 224.4
Description: Purified native Mouse Serum Albumin

Mouse Complement Serum
32R-CM001	Fitzgerald	5 ml	EUR 522
Description: Frozen high activity mouse complement serum

Normal Mouse Serum
88-NM35	Fitzgerald	50 ml	EUR 619.2
Description: Normal Mouse Serum

Normal Mouse Serum
88R-1002	Fitzgerald	5 ml	EUR 243.6
Description: Normal Mouse Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Mouse Free Tri-iodothyronine, Free-T3 ELISA Kit
CSB-E05077m-24T	Cusabio	1 plate of 24 wells	EUR 198
Description: Quantitative competitive ELISA kit for measuring Mouse Free Tri-iodothyronine, Free-T3 in samples from serum, plasma, cell culture supernates, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Mouse Free Tri-iodothyronine, Free-T3 ELISA Kit
1-CSB-E05077m	Cusabio	EUR 600.00 EUR 4082.40 EUR 2184.00	1 plate of 96 wells 10 plates of 96 wells each 5 plates of 96 wells each
Description: Quantitative competitive ELISA kit for measuring Mouse Free Tri-iodothyronine, Free-T3 in samples from serum, plasma, cell culture supernates, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Human IgG-Biotin (serum origin, purified >97%, low endotoxin, azide free)
20007-1-LE-BTN	Alpha Diagnostics	100 ug	EUR 343.2

Human Male AB Serum (Converted from Plasma), Xeno-Free Heat Inactivated
AR1006-0100	Akron Biotech	100mL	Ask for price

Bovine Serum Albumin ? Cohn Fraction V, Fatty Acid Free, pH ? 7.0
7907-100	Biovision	each	EUR 783.6

Bovine Serum Albumin ? Cohn Fraction V, Fatty Acid Free, pH ? 7.0
7907-25	Biovision	each	EUR 294

Bovine Serum Albumin ? Cohn Fraction V, Fatty Acid Free, pH ? 7.0
7907-5	Biovision	each	EUR 144

Bovine Serum Albumin ? Cohn Fraction V, Fatty Acid Free, pH ? 5.2
7908-100	Biovision	each	EUR 783.6

Bovine Serum Albumin ? Cohn Fraction V, Fatty Acid Free, pH ? 5.2
7908-25	Biovision	each	EUR 294

Bovine Serum Albumin ? Cohn Fraction V, Fatty Acid Free, pH ? 5.2
7908-5	Biovision	each	EUR 144

Mouse Serum Amyloid P Component (SAP) Reference serum
SAP12-RS	Alpha Diagnostics	100 ul	EUR 388.8

Mouse Free Tri-iodothyronine Indes,Free-T3 ELISA Kit
CN-02860M1	ChemNorm	96T	EUR 571.2

Mouse Free Tri-iodothyronine Indes,Free-T3 ELISA Kit
CN-02860M2	ChemNorm	48T	EUR 391.2

Mouse Free Tri-iodothyronine Indes(Free-T3)ELISA Kit
GA-E0078MS-48T	GenAsia Biotech	48T	EUR 403.2

Mouse Free Tri-iodothyronine Indes(Free-T3)ELISA Kit
GA-E0078MS-96T	GenAsia Biotech	96T	EUR 640.8

Mouse Free Tri-iodothyronine Indes(Free-T3)ELISA Kit
QY-E20615	Qayee Biotechnology	96T	EUR 433.2

Mouse Free Tri-iodothyronine Indes,Free-T3 ELISA Kit
YLA0389MO-48T	Shanghai YL Biotech	48T	EUR 465

Mouse Free Tri-iodothyronine Indes,Free-T3 ELISA Kit
YLA0389MO-96T	Shanghai YL Biotech	96T	EUR 600

Mouse Serum albumin (Alb)
1-CSB-YP001561MO	Cusabio	EUR 703.20 EUR 358.80 EUR 2606.40 EUR 1080.00 EUR 1730.40 EUR 458.40	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Mouse Serum albumin(Alb) expressed in Yeast

Mouse Serum albumin (Alb)
1-CSB-BP001561MO	Cusabio	EUR 1008.00 EUR 398.40 EUR 2604.00 EUR 1362.00 EUR 1894.80 EUR 564.00	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Mouse Serum albumin(Alb) expressed in Baculovirus

Mouse Serum Albumin antibody
70R-AG001	Fitzgerald	1 mg	EUR 307.2
Description: Affinity purified Goat polyclonal Mouse Serum Albumin antibody

Mouse Serum Albumin antibody
20R-AR005	Fitzgerald	20 mg	EUR 392.4
Description: Rabbit polyclonal Mouse Serum Albumin antibody

Mouse Serum albumin antibody
20R-AR041	Fitzgerald	2 ml	EUR 354
Description: Rabbit polyclonal Mouse Serum albumin antibody

Mouse Serum Albumin protein
30-1179	Fitzgerald	10 mg	EUR 283.2
Description: Purified native Mouse Albumin protein

Mouse Serum Albumin Protein
20-abx600021	Abbexa	EUR 1111.20 EUR 376.80 EUR 4770.00 EUR 292.80	100 mg 10 mg 1 g 1 mg

Non-sterile mouse serum
MS05-0050	Equitech	50 ml	EUR 277.68

Non-sterile mouse serum
MS05-0100	Equitech	100 ml	EUR 308.88

Non-sterile mouse serum
MS05-0500	Equitech	500 ml	EUR 528.84

IPTG, Animal-Free (Dioxane-Free)
1649-1G	Biovision	each	EUR 138

IPTG, Animal-Free (Dioxane-Free)
1649-50G	Biovision	each	EUR 1182

IPTG, Animal-Free (Dioxane-Free)
1649-5G	Biovision	each	EUR 261.6

Purified rat serum albumin protein (RSA, >99%, fatty acid and globulin free)
ALBR14-N-10	Alpha Diagnostics	10 mg	EUR 416.4

Bovine Serum Albumin ? Cohn Fraction V, Immunoassay Grade, Protease Free, pH ? 7.0
7906-100	Biovision	each	EUR 783.6

Bovine Serum Albumin ? Cohn Fraction V, Immunoassay Grade, Protease Free, pH ? 7.0
7906-25	Biovision	each	EUR 294

Bovine Serum Albumin ? Cohn Fraction V, Immunoassay Grade, Protease Free, pH ? 7.0
7906-5	Biovision	each	EUR 144

Bovine Serum Albumin ? Cohn Fraction V, Immunoassay Grade, Protease Free, pH ? 5.2
7911-100	Biovision	each	EUR 783.6

Bovine Serum Albumin ? Cohn Fraction V, Immunoassay Grade, Protease Free, pH ? 5.2
7911-25	Biovision	each	EUR 294

Bovine Serum Albumin ? Cohn Fraction V, Immunoassay Grade, Protease Free, pH ? 5.2
7911-5	Biovision	each	EUR 144

Amersham ECL Western Blotting Detection Reagents for 4000cm2 membrane
RPN2106	Scientific Laboratory Supplies	EACH	EUR 314.64

Amersham ECL Western Blotting Detection Reagents for 1000cm2 membrane
RPN2109	Scientific Laboratory Supplies	EACH	EUR 158.46

Amersham ECL Western Blotting Detection Reagents for 6000cm2 membrane
RPN2134	Scientific Laboratory Supplies	1KIT	EUR 482.22

Amersham ECL Western Blotting Detection Reagents for 2000cm2 membrane
RPN2209	Scientific Laboratory Supplies	EACH	EUR 245.1

Mouse Free cholesterol ELISA kit
E03F0212-192T	BlueGene	192 tests	EUR 1524
Description: A competitive ELISA for quantitative measurement of Mouse Free cholesterol in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Free cholesterol ELISA kit
E03F0212-48	BlueGene	1 plate of 48 wells	EUR 624
Description: A competitive ELISA for quantitative measurement of Mouse Free cholesterol in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Free cholesterol ELISA kit
E03F0212-96	BlueGene	1 plate of 96 wells	EUR 822
Description: A competitive ELISA for quantitative measurement of Mouse Free cholesterol in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Cobalt-CatalyzedDefluorosilylation of Aryl Fluorides via Grignard Reagent Formation

Transition-metal-catalyzed transformations of the carbon-fluorine bond not only tackle an interesting problem of challenging bond activation but also offer new synthetic strategies where the relatively inert C-F bond is converted to versatile functional groups. Herein we report a practical cobalt-catalyzedsilylation of aryl fluorides that uses a cheap electrophilic silicon source with magnesium. This method is compatible with various silicon sources and can be operated under aerobic conditions. Mechanistic studies support the in situ formation of a Grignard reagent, which is captured by the electrophilic silicon source.

A DAPI-Based Reagent for Mass Cytometry

Multiparametric single cell analysis has seen dramatic improvements with the introduction of Mass Cytometry (MC) and Imaging Mass Cytometry (IMC™). These technologies expanded the number of biomarkers that can be identified simultaneously by using heavy isotope-tagged antibody reagents. Small molecule probes bearing heavy isotopes are emerging as additional useful functional reporters of cellular features.

Realizing this, we explored the iodination of DAPI to produce a heavy atom substituted derivative of the commonly used fluorescent DNA stain. Although exhibiting a drastically reduced fluorescence emission profile, I-DAPI retains strong binding affinity for DNA. I-DAPI was used to detect cellular DNA in MC and IMC™ assays with comparable efficiency to known Ir containing DNA intercalators. This work suggests repurposing of well-known colorimetric stains through simple reactions could be an effective strategy to develop new, functional MC and IMC™ reagents.

Cautionary note on contamination of reagents used for molecular detection of SARS-CoV-2

Reverse transcription (RT)-PCR, the principal diagnostic method applied in the world-wide struggle against COVID-19, is capable of detecting a single molecule of a viral genome. Correctly designed and practiced RT-PCR assays for SARS-CoV-2 should not cross react with similar but distinct viral pathogens, such as the coronaviruses associated with the common cold, and should perform with very high analytical sensitivity. This analytical performance is predicated on the ability of the method to detect the presence of the selected nucleic acid target, without detection of a false positive signal.

Influence of reagent lots and multiple measuring systems on estimating the coefficient of variation from quality control data; implications for uncertainty estimation and interpretation of QC results

• AbstractBackgroundClinical laboratories use internal quality control (QC) data to calculate standard deviation (SD) and coefficient of variation (CV) to estimate uncertainty of results and to interpret QC results. We examined the influence of different instruments, and QC and reagent lots on the CV calculated from QC data.MethodsResults for BioRadMultiqual frozen liquid QC samples over a 2-year interval were partitioned by QC and reagent lots.
• The mean and CV were calculated for each partition for each of three Abbott Architect c8000 instruments for measuring serum alanine amino transferase (ALT), creatinine (enzymatic), glucose and sodium.ResultsCVs differed among partitions and instruments for two QC levels by 5.8- and 3.3-fold for ALT, by 4.7- and 2.1-fold for creatinine, by 2.0- and 2.6-fold for glucose, and by 2.1- and 2.0-fold for sodium. Pooled CVs for two QC levels varied among instruments by 1.78- and 1.11-fold for ALT, by 1.63- and 1.11-fold for creatinine, by 1.08- and 1.06-fold for glucose, and by 1.24- and 1.31-fold for sodium.
• ConclusionsThe CVs from QC data varied substantially among QC and reagent lots and for different identical specification instruments. The CV used to estimate uncertainty for a measurement result or as the basis for interpreting individual QC results must be derived over a sufficient time interval to obtain a pooled CV that represents “typical” performance of a measuring system. An estimate of uncertainty provided to users of laboratory results will itself have uncertainty that can influence medical decisions.

Human Free PSA (f-PSA) ELISA Kit
PRB-5049-FREE-5	Cell Biolabs	5 x 96 assays	EUR 2739.6

Human Serum (Troponin I Free)
90R-106	Fitzgerald	100 ml	EUR 1417.2
Description: Troponion I free normal human serum

Serum Albumin, Lipid Free Protein
20-abx260049	Abbexa	EUR 927.60 EUR 343.20 EUR 627.60	100 mg 10 mg 50 mg

Bovine Serum Albumin, protease free
GK4012-1KG	Glentham Life Sciences	1 kg	EUR 1165.2

Bovine Serum Albumin, protease free
GK4012-500G	Glentham Life Sciences	500 g	EUR 638.4

PK15 Cell Serum-Free Medium
VCum-Lsx0007	Creative Biolabs	1 L	EUR 909.6
Description: PK15 cell serum-free medium has been specifically developed for suspension culture of PK-15 cells.

CHO Cell Serum-Free Medium
VCum-Lsx0025	Creative Biolabs	1L	EUR 762
Description: A serum-free medium that is ideal for suspension culture of CHO cell lines (CHO DG44, CHO-K1 and CHO-S).

HEK Cell Serum-Free Medium
VCum-Lsx0027	Creative Biolabs	1L	EUR 762
Description: A serum-free medium that is ideal for suspension culture of HEK cell lines.

MDBK Cell Serum-Free Medium
VCum-Lsx0029	Creative Biolabs	1L	EUR 818.4
Description: Used in serum-free suspension MDBK cell line culture for bovine virus diarrhea or bovine rhinotracheitis vaccine production.

Vero Cell Serum-Free Medium
VCum-Lsx0031	Creative Biolabs	1 L	EUR 909.6
Description: A serum-free medium that is ideal for suspension culture of Vero cell lines.

500mL Insectagro DS2 Serum-Free
13-402-CV	Scientific Laboratory Supplies	PK6	EUR 222.3

500mL INSECTAGRO SF9 serum free
13-410-CV	Scientific Laboratory Supplies	PK6	EUR 192.66

EZ-DNA Reagents
BS8202	Bio Basic	100preps	EUR 105.67

EZ-DNA Reagents
SK8201	Bio Basic	100preps	EUR 112.2

Ecl Detection Reagents
RPN2105	Scientific Laboratory Supplies	EACH	EUR 611.04

Ecl Detection Reagents
RPN3004	Scientific Laboratory Supplies	EACH	EUR 489.06

B-27 Serum-Free Supplement (50x)
abx082466-10ml	Abbexa	10 ml	EUR 393.6

Normal Bovine Serum (Protease/IgG free)
88R-1012	Fitzgerald	10 ml	EUR 218.4
Description: Normal Bovine Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Fetal Bovine Serum, Premium Tetracyclin Free
F0500-050	GenDepot	500ml	EUR 1388.4

Bovine Serum Albumin, fatty acid free
GX5685-1KG	Glentham Life Sciences	1 kg	EUR 1442.4

Bovine Serum Albumin, fatty acid free
GX5685-500G	Glentham Life Sciences	500 g	EUR 792

ST Cell Serum-Free Medium-1
VCum-Lsx0001	Creative Biolabs	1 L	EUR 888
Description: ST cell serum-free medium has been specifically developed for suspension culture of ST cells.

ST Cell Serum-Free Medium-2
VCum-Lsx0003	Creative Biolabs	1 L	EUR 909.6
Description: ST cell serum-free medium has been specifically developed for suspension culture of ST cells.

PK15 Cell Serum-Free Medium (Powder)
VCum-Lsx0008-10L	Creative Biolabs	10 L	EUR 2798.4
Description: PK15 cell serum-free medium has been specifically developed for suspension culture of PK-15 cells.

PK15 Cell Serum-Free Medium (Powder)
VCum-Lsx0008-1L	Creative Biolabs	1 L	EUR 333.6
Description: PK15 cell serum-free medium has been specifically developed for suspension culture of PK-15 cells.

PK15 Cell Serum-Free Medium (Powder)
VCum-Lsx0008-5L	Creative Biolabs	5 L	EUR 1429.2
Description: PK15 cell serum-free medium has been specifically developed for suspension culture of PK-15 cells.

MDCK Cell Serum-Free Medium-1
VCum-Lsx0009	Creative Biolabs	1 L	EUR 818.4
Description: MDCK cell serum-free medium has been specifically developed for suspension culture of MDCK cells.

MDCK Cell Serum-Free Medium-2
VCum-Lsx0011	Creative Biolabs	1 L	EUR 832.8
Description: Used in serum-free suspension MDCK cell line culture for avian influenza vaccine production.

BHK Cell Serum-Free Medium-1
VCum-Lsx0017	Creative Biolabs	1 L	EUR 776.4
Description: BHK cell serum-free medium has been specifically developed for suspension culture of BHK cells.

BHK Cell Serum-Free Medium-2
VCum-Lsx0019	Creative Biolabs	1 L	EUR 860.4
Description: Used in serum-free suspension BHK cell line culture for newcastle disease virus production.

Insect Cell Serum-Free Medium-1
VCum-Lsx0021	Creative Biolabs	1 L	EUR 888
Description: A serum-free medium that is ideal for suspension culture of insect cell lines (HighFive, SF9).

Insect Cell Serum-Free Medium-2
VCum-Lsx0023	Creative Biolabs	1 L	EUR 909.6
Description: A serum-free medium that is ideal for suspension culture of insect cell lines (HighFive, SF9).

CHO Cell Serum-Free Medium (Powder)
VCum-Lsx0026-10L	Creative Biolabs	10 L	EUR 2096.4
Description: A serum-free medium that is ideal for suspension culture of CHO cell lines (CHO DG44, CHO-K1 and CHO-S).

CHO Cell Serum-Free Medium (Powder)
VCum-Lsx0026-1L	Creative Biolabs	1 L	EUR 264
Description: A serum-free medium that is ideal for suspension culture of CHO cell lines (CHO DG44, CHO-K1 and CHO-S).

CHO Cell Serum-Free Medium (Powder)
VCum-Lsx0026-5L	Creative Biolabs	5 L	EUR 1077.6
Description: A serum-free medium that is ideal for suspension culture of CHO cell lines (CHO DG44, CHO-K1 and CHO-S).

HEK Cell Serum-Free Medium (Powder)
VCum-Lsx0028	Creative Biolabs	50 L	EUR 7693.2
Description: A serum-free medium that is ideal for suspension culture of HEK cell lines.

MDBK Cell Serum-Free Medium (Powder)
VCum-Lsx0030	Creative Biolabs	50 L	EUR 10309.2
Description: Used in serum-free suspension MDBK cell line culture for bovine virus diarrhea or bovine rhinotracheitis vaccine production.

Vero Cell Serum-Free Medium (Powder)
VCum-Lsx0032-10L	Creative Biolabs	10 L	EUR 2798.4
Description: A serum-free medium that is ideal for suspension culture of Vero cell lines.

Vero Cell Serum-Free Medium (Powder)
VCum-Lsx0032-1L	Creative Biolabs	1 L	EUR 338.4
Description: A serum-free medium that is ideal for suspension culture of Vero cell lines.

Vero Cell Serum-Free Medium (Powder)
VCum-Lsx0032-5L	Creative Biolabs	5 L	EUR 1449.6
Description: A serum-free medium that is ideal for suspension culture of Vero cell lines.

Corning Lymphocyte Serum Free Medium KBM551
88-551-CM	Scientific Laboratory Supplies	1L	EUR 182.4

Corning Lymphocyte Serum Free Medium KBM581
88-581-CM	Scientific Laboratory Supplies	1L	EUR 240.54

REAGENTS HIGH RANGE AMMONIA
HI73325	Scientific Laboratory Supplies	PK25	EUR 38.19

High Range Ammonia Reagents
HI9373301	Scientific Laboratory Supplies	EACH	EUR 91.2

Alkphos Direct Labelling Reagents
RPN3680	Scientific Laboratory Supplies	EACH	EUR 686.28

Purified rat serum albumin protein (RSA, >99%, globulin free)
ALBR13-N-10	Alpha Diagnostics	10 mg	EUR 343.2

Human Apolipoprotein AI / B Free Serum Protein
abx060869-100ml	Abbexa	100 ml	EUR 594

ST Cell Serum-Free Medium-1 (Powder)
VCum-Lsx0002-10L	Creative Biolabs	10 L	EUR 2658
Description: ST cell serum-free medium has been specifically developed for suspension culture of ST cells.

ST Cell Serum-Free Medium-1 (Powder)
VCum-Lsx0002-1L	Creative Biolabs	1 L	EUR 319.2
Description: ST cell serum-free medium has been specifically developed for suspension culture of ST cells.

ST Cell Serum-Free Medium-1 (Powder)
VCum-Lsx0002-5L	Creative Biolabs	5 L	EUR 1358.4
Description: ST cell serum-free medium has been specifically developed for suspension culture of ST cells.

ST Cell Serum-Free Medium-2 (Powder)
VCum-Lsx0004-10L	Creative Biolabs	10 L	EUR 2798.4
Description: ST cell serum-free medium has been specifically developed for suspension culture of ST cells.

ST Cell Serum-Free Medium-2 (Powder)
VCum-Lsx0004-1L	Creative Biolabs	1 L	EUR 333.6
Description: ST cell serum-free medium has been specifically developed for suspension culture of ST cells.

ST Cell Serum-Free Medium-2 (Powder)
VCum-Lsx0004-5L	Creative Biolabs	5 L	EUR 1429.2
Description: ST cell serum-free medium has been specifically developed for suspension culture of ST cells.

MDCK Cell Serum-Free Medium-1 (Powder)
VCum-Lsx0010-10L	Creative Biolabs	10 L	EUR 2236.8
Description: MDCK cell serum-free medium has been specifically developed for suspension culture of MDCK cells.

MDCK Cell Serum-Free Medium-1 (Powder)
VCum-Lsx0010-1L	Creative Biolabs	1 L	EUR 277.2
Description: MDCK cell serum-free medium has been specifically developed for suspension culture of MDCK cells.

MDCK Cell Serum-Free Medium-1 (Powder)
VCum-Lsx0010-5L	Creative Biolabs	5 L	EUR 1148.4
Description: MDCK cell serum-free medium has been specifically developed for suspension culture of MDCK cells.

MDCK Cell Serum-Free Medium-2 (Powder)
VCum-Lsx0012-10L	Creative Biolabs	10 L	EUR 2306.4
Description: Used in serum-free suspension MDCK cell line culture for avian influenza vaccine production.

MDCK Cell Serum-Free Medium-2 (Powder)
VCum-Lsx0012-1L	Creative Biolabs	1 L	EUR 284.4
Description: Used in serum-free suspension MDCK cell line culture for avian influenza vaccine production.

MDCK Cell Serum-Free Medium-2 (Powder)
VCum-Lsx0012-5L	Creative Biolabs	5 L	EUR 1183.2
Description: Used in serum-free suspension MDCK cell line culture for avian influenza vaccine production.

BHK Cell Serum-Free Medium-1 (Powder)
VCum-Lsx0018-10L	Creative Biolabs	10 L	EUR 2025.6
Description: BHK cellserum-free medium has been specifically developed for suspension culture of BHK cells.

BHK Cell Serum-Free Medium-1 (Powder)
VCum-Lsx0018-1L	Creative Biolabs	1 L	EUR 256.8
Description: BHK cellserum-free medium has been specifically developed for suspension culture of BHK cells.

BHK Cell Serum-Free Medium-1 (Powder)
VCum-Lsx0018-5L	Creative Biolabs	5 L	EUR 1042.8
Description: BHK cellserum-free medium has been specifically developed for suspension culture of BHK cells.

BHK Cell Serum-Free Medium-2 (Powder)
VCum-Lsx0020-10L	Creative Biolabs	10 L	EUR 2306.4
Description: Used in serum-free suspension BHK cell line culture for newcastle disease virus production.

BHK Cell Serum-Free Medium-2 (Powder)
VCum-Lsx0020-1L	Creative Biolabs	1 L	EUR 284.4
Description: Used in serum-free suspension BHK cell line culture for newcastle disease virus production.

BHK Cell Serum-Free Medium-2 (Powder)
VCum-Lsx0020-5L	Creative Biolabs	5 L	EUR 1183.2
Description: Used in serum-free suspension BHK cell line culture for newcastle disease virus production.

Insect Cell Serum-Free Medium-1 (Powder)
VCum-Lsx0022-10L	Creative Biolabs	10 L	EUR 2658
Description: A serum-free medium that is ideal for suspension culture of insect cell lines (HighFive, SF9).

Insect Cell Serum-Free Medium-1 (Powder)
VCum-Lsx0022-1L	Creative Biolabs	1 L	EUR 319.2
Description: A serum-free medium that is ideal for suspension culture of insect cell lines (HighFive, SF9).

Insect Cell Serum-Free Medium-1 (Powder)
VCum-Lsx0022-5L	Creative Biolabs	5 L	EUR 1358.4
Description: A serum-free medium that is ideal for suspension culture of insect cell lines (HighFive, SF9).

Insect Cell Serum-Free Medium-2 (Powder)
VCum-Lsx0024-10L	Creative Biolabs	10 L	EUR 2798.4
Description: A serum-free medium that is ideal for suspension culture of insect cell lines (HighFive, SF9).

Insect Cell Serum-Free Medium-2 (Powder)
VCum-Lsx0024-1L	Creative Biolabs	1 L	EUR 333.6
Description: A serum-free medium that is ideal for suspension culture of insect cell lines (HighFive, SF9).

Insect Cell Serum-Free Medium-2 (Powder)
VCum-Lsx0024-5L	Creative Biolabs	5 L	EUR 1429.2
Description: A serum-free medium that is ideal for suspension culture of insect cell lines (HighFive, SF9).

CryoProtX Mix Reagents 1.5 mL
M-MDSR-61	MiTeGen	1.5 ml ml	EUR 37
Description: CryoProtX Mix Reagents 1.5 mL

Set of 10 Biolipidure Reagents
Biolipidure-set	AS ONE International	10mLx10	EUR 1820.4
Description: Set of 10 Biolipidure Reagents, whose applications include Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement

Custom production of antibodies in 5 Rats using customer supplied antigen (std 63 days protocol)
RAT-5	Alpha Diagnostics	1	EUR 1365.6

Insect Cell Medium: Serum-Free Insect Culture Medium
ABP-MED-10002	Allele Biotech	1 liter	Ask for price

Bovine serum albumin (BSA) protein (>99%, Protease-free)
BSA16-N-100	Alpha Diagnostics	100 mg	EUR 343.2

HSA Human Serum Albumin Recombinant Protein, Lipid Free
PROTP02768-3	BosterBio	Regular: 50mg	EUR 565.2
Description: HSA Human Recombinant lipid reduced produced in Plant is a non-glycosylated, polypeptide chain containing 585 amino acids and having a molecular mass of 67 kDa.

Exo-Flow 2.0 Basic Kit without antibody (Streptavidin beads + reagents) - for Serum or Plasma
EXOFLOW2-BASICA-SP	SBI	30 rxn	EUR 1201.2

Rat Serum
abx098267-10ml	Abbexa	10 ml	EUR 326.4

Bradford(Coomassie) Protein Assay Plus Reagents
P7201-050	GenDepot	450ml	EUR 225.6

CryoProtX Mix Eco Reagents 1.5 mL
M-MDSR-61-ECO	MiTeGen	1.5 ml ml	EUR 37
Description: CryoProtX Mix Eco Reagents 1.5 mL

Human Male AB Serum (Converted from Plasma), Xeno-Free
AR1005-0100	Akron Biotech	100mL	Ask for price

Rabbit control serum (non-immunized, Disease free, SPF Rabbits)
NSPF-1	Alpha Diagnostics	1 ml	EUR 343.2

Rabbit control serum (non-immunized, Disease free, SPF Rabbits)
NSPF-10	Alpha Diagnostics	10 ml	EUR 781.2

Rabbit control serum (non-immunized, Disease free, SPF Rabbits)
NSPF-5	Alpha Diagnostics	5 ml	EUR 634.8

Bovine Serum Albumin ? Heat Shock, Protease Free, pH 7.0
7919-100	Biovision	each	EUR 783.6

Bovine Serum Albumin ? Heat Shock, Protease Free, pH 7.0
7919-25	Biovision	each	EUR 294