On-tissue derivatization with Girard’s reagent

On-tissue derivatization with Girard’s reagent P enhances N-glycan signals for formalin-fixed paraffin-embedded tissue sections in MALDI mass spectrometry imaging

Glycosylation is a major protein post-translational modification whose dysregulation has been associated with many diseases. Herein, an on-tissue chemical derivatization strategy based on positively charged hydrazine reagent (Girard’s reagent P) coupled with matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) was developed for analysis of N-glycans from FFPE treated tissue sections.

The performance of the proposed approach was evaluated by analysis of monosaccharides, oligosaccharides, N-glycans released from glycoproteins, as well as MS imaging of N-glycans from human cancer tissue sections. The results demonstrated that the signal-to-noise ratios for target saccharides were notably improved after chemical derivatization, in which signals were enhanced by 230-fold for glucose and over 28-fold for maltooctaose. Improved glycome coverage was obtained for N-glycans derived from glycoproteins and tissue samples after chemical derivatization.

Furthermore, on-tissue derivatization was applied for MALDI-MSI of N-glycans from human laryngeal cancer and ovarian cancer tissues. Differentially expressed N-glycans among the tumor region, adjacent normal tissue region, and tumor proximal collagen stroma region were imaged, revealing that high-mannose type N-glycans were predominantly expressed in the tumor region. Overall, our results indicate that the on-tissue labeling strategy coupled with MALDI-MSI shows great potential to spatially characterize N-glycan expression within heterogeneous tissue samples with enhanced sensitivity. This study provides a promising approach to better understand the pathogenesis of cancer related aberrant glycosylation, which is beneficial to the design of improved clinical diagnosis and therapeutic strategies.

 

RAT SERUM WITH 0.09% AZIDE
MBS238236-100mL	MyBiosource	100mL	EUR 2170

RAT SERUM WITH 0.09% AZIDE
MBS238236-5x100mL	MyBiosource	5x100mL	EUR 9600

MOUSE SERUM WITH 0.09% AZIDE
MBS238221-5mL	MyBiosource	5mL	EUR 190

MOUSE SERUM WITH 0.09% AZIDE
MBS238221-5x5mL	MyBiosource	5x5mL	EUR 680

MOUSE SERUM WITH 0.09% AZIDE
MBS238222-10mL	MyBiosource	10mL	EUR 250

MOUSE SERUM WITH 0.09% AZIDE
MBS238222-5x10mL	MyBiosource	5x10mL	EUR 960

HORSE SERUM WITH 0.09% AZIDE
MBS239299-100mL	MyBiosource	100mL	EUR 190

HORSE SERUM WITH 0.09% AZIDE
MBS239299-5x100mL	MyBiosource	5x100mL	EUR 675

RAT SERUM WITH 0.09% AZIDE Serum Products
GWB-Q00021	GenWay Biotech	10 ml	Ask for price

RAT SERUM WITH 0.09% AZIDE Serum Products
GWB-Q00022	GenWay Biotech	50 ml	Ask for price

RAT SERUM WITH 0.09% AZIDE Serum Products
GWB-Q00023	GenWay Biotech	100 ml	Ask for price

MOUSE SERUM WITH 0.09% AZIDE Serum Products
GWB-Q00007	GenWay Biotech	5 ml	Ask for price

MOUSE SERUM WITH 0.09% AZIDE Serum Products
GWB-Q00008	GenWay Biotech	10 ml	Ask for price

MOUSE SERUM WITH 0.09% AZIDE Serum Products
GWB-Q00009	GenWay Biotech	50 ml	Ask for price

GUINEA PIG SERUM WITH 0.09% AZIDE Serum Products
GWB-Q00004	GenWay Biotech	5 ml	Ask for price

OOSA10412-5ML - SERUM WITH 0.09% AZIDE Serum & Cells
OOSA10412-5ML	Aviva Systems Biology	5ml	EUR 69

OOSA10414-10ML - SERUM WITH 0.09% AZIDE Serum & Cells
OOSA10414-10ML	Aviva Systems Biology	10ml	EUR 149

OOSA10429-10ML - Rat SERUM WITH 0.09% AZIDE Serum & Cells
OOSA10429-10ML	Aviva Systems Biology	10ml	EUR 279

OOSA10433-100ML - Rat SERUM WITH 0.09% AZIDE Serum & Cells
OOSA10433-100ML	Aviva Systems Biology	100ml	EUR 1799

Goat Serum (10%) in PBST with Azide
30611260-1	Bio-WORLD	100 ml	EUR 62.55

Goat Serum (10%) in PBST with Azide
30611260-2	Bio-WORLD	250 ml	EUR 117.74

Horse Serum (10%) in PBST with Azide
30611278-1	Bio-WORLD	100 ml	EUR 62.91

Horse Serum (10%) in PBST with Azide
30611278-2	Bio-WORLD	250 ml	EUR 89.78

Goat Serum (10%) in TBST Buffer with Azide
30611263-1	Bio-WORLD	100 mL	EUR 62.55

Goat Serum (10%) in TBST Buffer with Azide
30611263-2	Bio-WORLD	250 ml	EUR 116.81

Horse Serum (10%) in PBS with Azide, pH 7.4
40120170-1	Bio-WORLD	100 ml	EUR 62.91

Horse Serum (10%) in PBS with Azide, pH 7.4
40120170-2	Bio-WORLD	250 ml	EUR 89.78

Goat Serum (10%) in TBST Wash Buffer with Azide
30611266-1	Bio-WORLD	100 ml	EUR 62.55

Goat Serum (10%) in TBST Wash Buffer with Azide
30611266-2	Bio-WORLD	250 ml	EUR 116.81

Rabbit IgG, purified (serum non-immune, isotype control), without azide, powder
20009-WA-1	Alpha Diagnostics	1 mg	EUR 169.2

Rabbit IgG, purified (serum non-immune, isotype control), without azide, powder
20009-WA-5	Alpha Diagnostics	5 mg	EUR 343.2

Sheep Serum (10%) in PBST with Azide, pH 7.4, Sterile
30611251-1	Bio-WORLD	100 ml	EUR 40.4

Sheep Serum (10%) in PBST with Azide, pH 7.4, Sterile
30611251-2	Bio-WORLD	250 ml	EUR 73.55

Horse Serum (10%) in TBST with Sodium Azide, Sterile
30611281-1	Bio-WORLD	100 ml	EUR 62.91

Horse Serum (10%) in TBST with Sodium Azide, Sterile
30611281-2	Bio-WORLD	250 ml	EUR 89.78

Sheep Serum (10%) in TBST Wash Buffer with Azide, Sterile
30611257-1	Bio-WORLD	100 ml	EUR 40.97

Sheep Serum (10%) in TBST Wash Buffer with Azide, Sterile
30611257-2	Bio-WORLD	250 ml	EUR 73.55

Donkey Serum (10%) in TBST Wash Buffer with Azide, Sterile
30611275-1	Bio-WORLD	50 ml	EUR 45.09

Donkey Serum (10%) in TBST Wash Buffer with Azide, Sterile
30611275-2	Bio-WORLD	100 ml	EUR 66.89

Sheep Serum (10%) in TBST Standard Buffer with Azide, Sterile
30611254-1	Bio-WORLD	100 ml	EUR 40.97

Sheep Serum (10%) in TBST Standard Buffer with Azide, Sterile
30611254-2	Bio-WORLD	250 ml	EUR 73.55

Donkey Serum (10%) in TBST Standard Buffer with Azide, Sterile
30611272-1	Bio-WORLD	50 ml	EUR 38.84

Donkey Serum (10%) in TBST Standard Buffer with Azide, Sterile
30611272-2	Bio-WORLD	100 ml	EUR 67.01

RABBIT SERUM:Azide Free Serum Products
GWB-Q00019	GenWay Biotech	20 ml	Ask for price

RABBIT SERUM:Azide Free Serum Products
GWB-Q00020	GenWay Biotech	100 ml	Ask for price

Horse Serum (10%) in TBST Wash Buffer with Sodium Azide, Sterile
30611283-1	Bio-WORLD	100 ml	EUR 62.91

Horse Serum (10%) in TBST Wash Buffer with Sodium Azide, Sterile
30611283-2	Bio-WORLD	250 ml	EUR 89.78

Rabbit polyclonal CD29 antibody (with azide)
MBS5305515-01mL	MyBiosource	0.1mL	EUR 1410

Rabbit polyclonal CD29 antibody (with azide)
MBS5305515-5x01mL	MyBiosource	5x0.1mL	EUR 6190

Monoclonal BSA (Bovine Serum Albumin) Antibody - With BSA and Azide, Clone: SPM376
APR07282G	Leading Biology	0.05mg	EUR 475.2
Description: A Monoclonal antibody against Human BSA (Bovine Serum Albumin) - With BSA and Azide. The antibodies are raised in Mouse and are from clone SPM376. This antibody is applicable in E

Monoclonal BSA (Bovine Serum Albumin) Antibody - With BSA and Azide, Clone: ALB/398
APR07283G	Leading Biology	0.05mg	EUR 475.2
Description: A Monoclonal antibody against Human BSA (Bovine Serum Albumin) - With BSA and Azide. The antibodies are raised in Mouse and are from clone ALB/398. This antibody is applicable in E

Goat anti Rabbit Serum proteins, conjugated with FITC
MBS573568-1mL	MyBiosource	1mL	EUR 325

Goat anti Rabbit Serum proteins, conjugated with FITC
MBS573568-5x1mL	MyBiosource	5x1mL	EUR 1320

Rabbit anti Dog Serum proteins, conjugated with FITC
MBS573657-1mL	MyBiosource	1mL	EUR 325

Rabbit anti Dog Serum proteins, conjugated with FITC
MBS573657-5x1mL	MyBiosource	5x1mL	EUR 1320

Albumin, Human Serum (Azide Free) (HSA)
MBS6120697-025mg	MyBiosource	0.25(mg	EUR 495

Albumin, Human Serum (Azide Free) (HSA)
MBS6120697-1mg	MyBiosource	1mg	EUR 1000

Albumin, Human Serum (Azide Free) (HSA)
MBS6120697-5x1mg	MyBiosource	5x1mg	EUR 4350

ALB (ALB, Serum albumin) (Azide free) (HRP)
MBS6121446-02mL	MyBiosource	0.2(mL	EUR 980

ALB (ALB, Serum albumin) (Azide free) (HRP)
MBS6121446-5x02mL	MyBiosource	5x0.2mL	EUR 4250

Albumin, Human Serum (BSA) (BSA & Azide Free)
MBS6120698-025mg	MyBiosource	0.25(mg	EUR 445

Albumin, Human Serum (BSA) (BSA & Azide Free)
MBS6120698-1mg	MyBiosource	1mg	EUR 1015

Albumin, Human Serum (BSA) (BSA & Azide Free)
MBS6120698-5x1mg	MyBiosource	5x1mg	EUR 4415

Monoclonal BSA (Bovine Serum Albumin) Antibody - Without BSA and Azide, Clone: SPM376
APR07284G	Leading Biology	0.1mg	EUR 580.8
Description: A Monoclonal antibody against Human BSA (Bovine Serum Albumin) - Without BSA and Azide. The antibodies are raised in Mouse and are from clone SPM376. This antibody is applicable in E

Monoclonal BSA (Bovine Serum Albumin) Antibody - Without BSA and Azide, Clone: ALB/398
AMM04382G	Leading Biology	0.1mg	EUR 580.8
Description: A Monoclonal antibody against Human BSA (Bovine Serum Albumin) - Without BSA and Azide. The antibodies are raised in Mouse and are from clone ALB/398. This antibody is applicable in E

Rabbit Serum
1267-1	Biovision	each	EUR 188.4

Rabbit Serum
abx810190-10nmol	Abbexa	10 nmol	EUR 287.5

Rabbit Serum
abx810190-5nmol	Abbexa	5 nmol	EUR 250

Rabbit Serum
abx098265-100ml	Abbexa	100 ml	EUR 326.4

Rabbit Serum
abx098266-100l	Abbexa	100 µl	EUR 2900

Rabbit Serum
abx098266-1ml	Abbexa	1 ml	Ask for price

Rabbit Serum
abx098266-200l	Abbexa	200 µl	Ask for price

Rabbit Serum
abx098266-500ml	Abbexa	500 ml	EUR 577.2

Rabbit serum
IHR-8142	ImmunoBioscience	20 ml	EUR 47.75

Rabbit Serum
MBS571974-1mL	MyBiosource	1mL	EUR 160

Rabbit Serum
MBS571974-5x1mL	MyBiosource	5x1mL	EUR 575

Rabbit Serum
MBS556469-500mL	MyBiosource	500mL	EUR 155

Rabbit Serum
MBS556469-5x500mL	MyBiosource	5x500mL	EUR 560

Rabbit Serum
SER003	Neuromics	each	EUR 46.8

Normal Rabbit Serum rabbit polyclonal antibody, Serum
AP08139SU-N	Origene Technologies GmbH	5 ml	Ask for price

Rabbit Anti-Sheep IgG (H+L)-FITC conjugate (adsorbed with multispecies serum)
30530-AD	Alpha Diagnostics	0.5 ml	EUR 255.6

Limited Tryptic Digestion-Isotope Dilution Mass Spectrometry (LTD-IDMS): A Reagent-Free Analytical Assay To Quantify Hemagglutinin of A(H5N1) Vaccine Material

• Avian influenza viruses, such as A(H5N1) and A(H7N9), are primary public health concerns due to their pandemic potential. Influenza vaccines represent the most effective response to this threat especially with timely provision. The current pandemic response timelines require a substantial period for strain-specific reference antigen and sera preparation for use with single-radial immunodiffusion (SRID), the accepted vaccine potency assay.
• To address this time lag, the isotope dilution mass spectrometry (IDMS) method was developed to quantify the absolute hemagglutinin (HA, the main influenza antigen) amount in the vaccine without the need for purified, inactivated, and calibrated virus reference antigens. However, an additional challenge in determining potency is to differentiate between vaccine antigens in their most potent form from other less potent, stressed antigen forms.
• The limited trypsin digestion (LTD) method has been developed and does not require strain-specific full-length reference antigens or antibodies; instead, stressed HA is selectively degraded, leaving the more potent form to be measured. LTD, followed by precipitation and IDMS, allows for efficient differentiation between potent and significantly less potent HA for vaccine release and potency testing across the vaccine’s shelf life.
• In this study, we tested the LTD-IDMS assay on A(H5N1) vaccine material that had been stressed by low pH, heat, and multiple freeze-thaw cycles. The results showed that the LTD-IDMS method effectively quantified the potent HA in A(H5N1) vaccine material with results comparable to SRID. As such, it shows great promise to complement and potentially replace SRID in a pandemic when strain-specific reagents may not be readily available.

Translation of ceragenin affinity for bacteria to an imaging reagent for infection

Responses to bacterial infections may be manifest systemically without evidence of the location of the infection site. A rapid means of pinpointing infection sites would be useful in providing effective and possibly localized treatment. Successful means of identifying infection sites would require two components: (1) a molecule capable of recognizing bacteria and (2) a means of communicating recognition.

For the recognition element, we used a ceragenin, a small molecule with affinity for bacterial membranes that was designed as a mimic of endogenous antimicrobial peptides. For the communication element, we used ⁶⁴Cu, which is a positron emitter. By conjugating a copper chelating group to the ceragenin, the two elements were combined. Chelation of ⁶⁴Cu by the conjugate was effective and provided a stable complex that allowed in vivo imaging.

When administered to mice in a thigh infection model, the ⁶⁴Cu-labeled conjugate accumulated at the site of infection (right thigh) without accumulation at the complementary site (left thigh). This conjugate may provide a means of identifying infection sites in patients presenting general signs of infection without localized symptoms.

Human Serum Albumin Antigen, Reagent Grade
MBS568775-5x100g	MyBiosource	5x100g	EUR 5385

Bovine Serum Albumin Fatty Acid Free Reagent Grade
GWB-F06E29	GenWay Biotech	1 kg	Ask for price

T-Pro Total Exosome Isolation reagent (from serum)
JO66-V002M	T-Pro Biotechnology	25ml/BT	EUR 800

T-Pro Total Exosome Isolation reagent (from serum)
JO66-V002S	T-Pro Biotechnology	1ml*5/set	EUR 200

Bovine Serum Albumin ? Heat Shock, Reagent Grade, pH 7.0
7917-100	Biovision	each	EUR 783.6

Bovine Serum Albumin ? Heat Shock, Reagent Grade, pH 7.0
7917-25	Biovision	each	EUR 294

Bovine Serum Albumin ? Heat Shock, Reagent Grade, pH 7.0
7917-5	Biovision	each	EUR 144

ExoQualiTM Overall Exosome Isolation Reagent (from serum)
CEIK-044L-30T	Creative Bioarray	30 T	Ask for price

ExoQualiTM Overall Exosome Isolation Reagent (from serum)
CEIK-044L-50T	Creative Bioarray	50 T	Ask for price

Albumin Depletion Reagent for Plasma and Serum (10 ml) NEW!
P5W13	101Bio	10 rxn	EUR 99

OPEF01351-100G - Human Serum Albumin Antigen Reagent Grade
OPEF01351-100G	Aviva Systems Biology	100g	EUR 1302

Bovine Serum Albumin Fatty Acid Protease Free Reagent Grade
GWB-DCC190	GenWay Biotech	1 kg	Ask for price

MinuteTM Albumin Depletion Reagent for Plasma and Serum (20 ml)
WA-013	Inventbiotech	each	EUR 135

MOUSE SERUM Serum Products
GWB-Q00006	GenWay Biotech	100 ml	Ask for price

Albumin Bovine (pH 7) fraction V Reagent Grade (Bovine Serum Albumin, BSA), 99%
65186	Sisco Laboratories	10 Gms	EUR 38.4
Description: Part B

EZ-DNA Reagents
SK8201	Bio Basic	100preps	EUR 112.2

Ecl Detection Reagents
RPN3004	Scientific Laboratory Supplies	EACH	EUR 552.9

Mouse Serum
abx098261-100L	Abbexa	100 L	EUR 600

Mouse Serum
20-abx098261	Abbexa	Ask for price Ask for price	10 ml 100 ml

Mouse Serum
abx098261-1ml	Abbexa	1 ml	Ask for price

Mouse Serum
abx098261-200l	Abbexa	200 µl	Ask for price

Mouse Serum
abx098261-5L	Abbexa	5 L	EUR 10599.6

Mouse Serum
abx098262-1ml	Abbexa	1 ml	EUR 2064

Mouse serum
IHR-8141	ImmunoBioscience	10 ml	EUR 47.75

Mouse Serum
M2021-050	GenDepot	500ml	EUR 970

MOUSE SERUM
MBS238224-100mL	MyBiosource	100mL	EUR 845

MOUSE SERUM
MBS238224-5x100mL	MyBiosource	5x100mL	EUR 3620

Mouse Serum - 100ml
MO-340/100	Biosera France - Medical Scientific Equipment	100ml	EUR 183.32

Mouse Serum - 500ml
MO-340/500	Biosera France - Medical Scientific Equipment	500ml	EUR 733.32

MOUSE SERUM WITH 0.09% AZIDE Serum Products
GWB-Q00007	GenWay Biotech	5 ml	Ask for price

MOUSE SERUM WITH 0.09% AZIDE Serum Products
GWB-Q00008	GenWay Biotech	10 ml	Ask for price

MOUSE SERUM WITH 0.09% AZIDE Serum Products
GWB-Q00009	GenWay Biotech	50 ml	Ask for price

Serum, Mouse
MBS638822-100mL	MyBiosource	100mL	EUR 815

Serum, Mouse
MBS638822-5x100mL	MyBiosource	5x100mL	EUR 3435

Ecl Detection Reagents - EACH
RPN2105	Scientific Laboratory Supplies	EACH	EUR 815.4

Mouse Serum Proteins goat polyclonal antibody, Serum
AP31483SU-N	Origene Technologies GmbH	1 ml	Ask for price

CryoProtX Mix Reagents 1.5 mL
M-MDSR-61	MiTeGen	1.5 ml ml	EUR 37
Description: CryoProtX Mix Reagents 1.5 mL

Mouse Serum Proteins rabbit polyclonal antibody, Serum
AP31472SU-N	Origene Technologies GmbH	1 ml	Ask for price

Set of 10 Biolipidure Reagents
Biolipidure-set	AS ONE International	10mLx10	EUR 1820.4
Description: Set of 10 Biolipidure Reagents, whose applications include Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement

REAGENTS HIGH RANGE AMMONIA - PK25
HI73325	Scientific Laboratory Supplies	PK25	EUR 55.35

High Range Ammonia Reagents - EACH
HI9373301	Scientific Laboratory Supplies	EACH	EUR 130.95

Mouse Serum Amyloid P Component (SAP) Reference serum
SAP12-RS	Alpha Diagnostics	100 ul	EUR 388.8

Mouse Serum pool
MBS571968-1mL	MyBiosource	1mL	EUR 160