On-tissue derivatization with Girard’s reagent

On-tissue derivatization with Girard’s reagent P enhances N-glycan signals for formalin-fixed paraffin-embedded tissue sections in MALDI mass spectrometry imaging

Glycosylation is a major protein post-translational modification whose dysregulation has been associated with many diseases. Herein, an on-tissue chemical derivatization strategy based on positively charged hydrazine reagent (Girard’s reagent P) coupled with matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) was developed for analysis of N-glycans from FFPE treated tissue sections.

The performance of the proposed approach was evaluated by analysis of monosaccharides, oligosaccharides, N-glycans released from glycoproteins, as well as MS imaging of N-glycans from human cancer tissue sections. The results demonstrated that the signal-to-noise ratios for target saccharides were notably improved after chemical derivatization, in which signals were enhanced by 230-fold for glucose and over 28-fold for maltooctaose. Improved glycome coverage was obtained for N-glycans derived from glycoproteins and tissue samples after chemical derivatization.

Furthermore, on-tissue derivatization was applied for MALDI-MSI of N-glycans from human laryngeal cancer and ovarian cancer tissues. Differentially expressed N-glycans among the tumor region, adjacent normal tissue region, and tumor proximal collagen stroma region were imaged, revealing that high-mannose type N-glycans were predominantly expressed in the tumor region. Overall, our results indicate that the on-tissue labeling strategy coupled with MALDI-MSI shows great potential to spatially characterize N-glycan expression within heterogeneous tissue samples with enhanced sensitivity. This study provides a promising approach to better understand the pathogenesis of cancer related aberrant glycosylation, which is beneficial to the design of improved clinical diagnosis and therapeutic strategies.

 

Rabbit Serum
abx098265-100ml	Abbexa	100 ml	EUR 272
Shipped within 5-10 working days.

Rabbit Serum
abx098266-500ml	Abbexa	500 ml	EUR 481
Shipped within 5-10 working days.

Rabbit Serum
1267-1	Biovision		EUR 157

Rabbit IgG, purified (serum non-immune, isotype control), without azide, powder
20009-WA-1	Alpha Diagnostics	1 mg	EUR 141

Rabbit IgG, purified (serum non-immune, isotype control), without azide, powder
20009-WA-5	Alpha Diagnostics	5 mg	EUR 286

Rabbit Serum (Sterile)
20-abx800002	Abbexa	EUR 1400.00 EUR 3293.00 EUR 829.00	100 ml 500 ml 50 ml
Shipped within 1-5 working days.

Rabbit Serum (Sterile)
abx800002-1L	Abbexa	1 L	EUR 5437
Shipped within 1-5 working days.

Normal Rabbit Serum
88-NR50	Fitzgerald	100 ml	EUR 150
Description: Normal Rabbit Serum

Normal Rabbit Serum
88R-1004	Fitzgerald	10 ml	EUR 206
Description: Normal Rabbit Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Normal Rabbit Serum
89R-RM001	Fitzgerald	20 mg	EUR 392
Description: Normal Rabbit Serum (Mouse cell adsorbed)

Normal Rabbit Serum
89R-RM002	Fitzgerald	5 ml	EUR 241
Description: Normal Rabbit Serum (Mouse cell adsorbed)

Normal Rabbit Serum
89R-RR001	Fitzgerald	20 mg	EUR 349
Description: Normal Rabbit Serum (Rat cell adsorbed)

Normal Rabbit Serum
89R-RR002	Fitzgerald	5 ml	EUR 241
Description: Normal Rabbit Serum (Rat cell adsorbed)

Rabbit Serum Albumin
30R-3303	Fitzgerald	10 mg	EUR 187
Description: Purified native Rabbit Serum Albumin

Rabbit Complement Serum
32R-CR001	Fitzgerald	25 ml	EUR 241
Description: Purifed Rabbit Complement isolated from blood of New Zealand white rabbits

Glycated Bovine Serum Albumin (BSA) with ?-Galactose
SPR-203A	Stressmarq	0.05 mg	EUR 310
Description: Untagged synthetic panspecies Galactose BSA Glycoconjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

Glycated Bovine Serum Albumin (BSA) with ?-Galactose
SPR-203B	Stressmarq	2x0.05 mg	EUR 457
Description: Untagged synthetic panspecies Galactose BSA Glycoconjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

Glycated Bovine Serum Albumin (BSA) with ?-Galactose
SPR-203C	Stressmarq	4x0.05 mg	EUR 653
Description: Untagged synthetic panspecies Galactose BSA Glycoconjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

Glycated Bovine Serum Albumin (BSA) with ?-Glucose
SPR-204A	Stressmarq	0.05 mg	EUR 310
Description: Untagged synthetic panspecies Glucose BSA Glycoconjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

Glycated Bovine Serum Albumin (BSA) with ?-Glucose
SPR-204B	Stressmarq	2x0.05 mg	EUR 457
Description: Untagged synthetic panspecies Glucose BSA Glycoconjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

Glycated Bovine Serum Albumin (BSA) with ?-Glucose
SPR-204C	Stressmarq	4x0.05 mg	EUR 653
Description: Untagged synthetic panspecies Glucose BSA Glycoconjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

Bovine Serum Albumin (BSA) modified with Acrolein
SPR-206A	Stressmarq	0.05 mg	EUR 256
Description: Untagged synthetic panspecies Acrolein BSA Conjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

Bovine Serum Albumin (BSA) modified with Acrolein
SPR-206B	Stressmarq	2x0.05 mg	EUR 370
Description: Untagged synthetic panspecies Acrolein BSA Conjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

Bovine Serum Albumin (BSA) modified with Acrolein
SPR-206C	Stressmarq	4x0.05 mg	EUR 523
Description: Untagged synthetic panspecies Acrolein BSA Conjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

Bovine Serum Albumin (BSA) modified with Crotonaldehyde
SPR-207A	Stressmarq	0.05 mg	EUR 310
Description: Untagged synthetic panspecies Crotonaldehyde BSA Conjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

Bovine Serum Albumin (BSA) modified with Crotonaldehyde
SPR-207B	Stressmarq	2x0.05 mg	EUR 457
Description: Untagged synthetic panspecies Crotonaldehyde BSA Conjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

Bovine Serum Albumin (BSA) modified with Crotonaldehyde
SPR-207C	Stressmarq	4x0.05 mg	EUR 653
Description: Untagged synthetic panspecies Crotonaldehyde BSA Conjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

Bovine Serum Albumin (BSA) modified with Methylglyoxal
SPR-208A	Stressmarq	0.05 mg	EUR 256
Description: Untagged synthetic panspecies Methylglyoxal BSA Conjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

Bovine Serum Albumin (BSA) modified with Methylglyoxal
SPR-208B	Stressmarq	2x0.05 mg	EUR 370
Description: Untagged synthetic panspecies Methylglyoxal BSA Conjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

Bovine Serum Albumin (BSA) modified with Methylglyoxal
SPR-208C	Stressmarq	4x0.05 mg	EUR 523
Description: Untagged synthetic panspecies Methylglyoxal BSA Conjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

Bovine Serum Albumin (BSA) modified with Malondialdehyde
SPR-209A	Stressmarq	0.05 mg	EUR 256
Description: Untagged synthetic panspecies Malondialdehyde BSA Conjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

Bovine Serum Albumin (BSA) modified with Malondialdehyde
SPR-209B	Stressmarq	2x0.05 mg	EUR 370
Description: Untagged synthetic panspecies Malondialdehyde BSA Conjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

Bovine Serum Albumin (BSA) modified with Malondialdehyde
SPR-209C	Stressmarq	4x0.05 mg	EUR 523
Description: Untagged synthetic panspecies Malondialdehyde BSA Conjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

Heat shock 30% solution, with NaCL and Azide
BAL62NA-0100	Equitech	100ml	EUR 236.6
Heat shock 30% solution, with NaCL and Azide is indicated as RUO. Do not use on humans.

Heat shock 30% solution, with NaCL and Azide
BAL62NA-0500	Equitech	500ml	EUR 432.9
Heat shock 30% solution, with NaCL and Azide is indicated as RUO. Do not use on humans.

Heat shock 30% solution, with NaCL and Azide
BAL62NA-1000	Equitech	1L	EUR 626.6
Heat shock 30% solution, with NaCL and Azide is indicated as RUO. Do not use on humans.

Rabbit Anti-Sheep IgG (H+L)-FITC conjugate (adsorbed with multispecies serum)
30530-AD	Alpha Diagnostics	0.5 ml	EUR 213

Cow Anti-Rabbit IgG-HRP conjugate (preadsorbed with mouse, human serum proteins)
20323-210	Alpha Diagnostics	0.5 ml	EUR 202

Rabbit Serum (Non-Sterile
20-abx800001	Abbexa	EUR 1080.00 EUR 2472.00 EUR 662.00	100 ml 500 ml 50 ml
Shipped within 1-5 working days.

Rabbit Serum (Non-Sterile
abx800001-1L	Abbexa	1 L	EUR 4281
Shipped within 1-5 working days.

Rabbit anti Pig Serum
40R-RS001	Fitzgerald	2 ml	EUR 241
Description: Rabbit anti Pig Serum antibody

Non-sterile rabbit serum
RS05-0100	Equitech	100 ml	EUR 166.4
Non-sterile rabbit serum is indicated as RUO. Do not use on humans.

Non-sterile rabbit serum
RS05-0500	Equitech	500ml	EUR 192.4
Non-sterile rabbit serum is indicated as RUO. Do not use on humans.

DiagPoly Amine Fluorescent Polystyrene Particles, Yellow, 0.09-0.3 µm
DNM-L056	Creative Diagnostics	2 mL	EUR 694

DiagPoly Carboxyl Fluorescent Polystyrene Particles, Yellow, 0.09-0.3 µm
DNM-L063	Creative Diagnostics	2 mL	EUR 658

DILUTION SOLUTION WITH HEPES, WITHOUT HUMAN SERUM ALBUMIN
99-783-CM	CORNING	1 L /pk	EUR 63
Description: Specialty Media; Islet products

WASH SOLUTION WITH HEPES, WITHOUT HUMAN SERUM ALBUMIN
99-784-CM	CORNING	1 L /pk	EUR 71
Description: Specialty Media; Islet products

Glycated Bovine Serum Albumin (BSA) with ?-N-Acetylglucosamine
SPR-201A	Stressmarq	0.05 mg	EUR 256
Description: Untagged synthetic panspecies N-Acetylglucosamine BSA Glycoconjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

Glycated Bovine Serum Albumin (BSA) with ?-N-Acetylglucosamine
SPR-201B	Stressmarq	2x0.05 mg	EUR 370
Description: Untagged synthetic panspecies N-Acetylglucosamine BSA Glycoconjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

Glycated Bovine Serum Albumin (BSA) with ?-N-Acetylglucosamine
SPR-201C	Stressmarq	4x0.05 mg	EUR 523
Description: Untagged synthetic panspecies N-Acetylglucosamine BSA Glycoconjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

Glycated Bovine Serum Albumin (BSA) with ?-N-Acetylgalactosamine
SPR-202A	Stressmarq	0.05 mg	EUR 310
Description: Untagged synthetic panspecies N-Acetylgalactosamine BSA Glycoconjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

Glycated Bovine Serum Albumin (BSA) with ?-N-Acetylgalactosamine
SPR-202B	Stressmarq	2x0.05 mg	EUR 457
Description: Untagged synthetic panspecies N-Acetylgalactosamine BSA Glycoconjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

Glycated Bovine Serum Albumin (BSA) with ?-N-Acetylgalactosamine
SPR-202C	Stressmarq	4x0.05 mg	EUR 653
Description: Untagged synthetic panspecies N-Acetylgalactosamine BSA Glycoconjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

Rabbit Anti-Sheep IgG (H+L)-Rhodamine/TRITC conjugate (adsorbed with multispecies serum)
30530-TR	Alpha Diagnostics	0.5 ml	EUR 213

Human IgG (serum origin, purified >97%, low endotoxin, azide free)
20007-1-LE-1	Alpha Diagnostics	1 mg	EUR 164

Polyclonal beta-Catenin (p120) Antibody - With BSA and Azide
APR00532G	Leading Biology	50 ug	EUR 396
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human beta-Catenin (p120) - With BSA and Azide. This antibody is tested and proven to work in the following applications:

Monoclonal Fibronectin Antibody - With BSA and Azide, Clone: SPM539
AMM00268G	Leading Biology	0.05mg	EUR 396
Description: A Monoclonal antibody against Human Fibronectin - With BSA and Azide. The antibodies are raised in Mouse and are from clone SPM539. This antibody is applicable in IHC-P, IF, FC

Monoclonal Fibronectin Antibody - With BSA and Azide, Clone: SPM246
AMM00271G	Leading Biology	0.05mg	EUR 396
Description: A Monoclonal antibody against Human Fibronectin - With BSA and Azide. The antibodies are raised in Mouse and are from clone SPM246. This antibody is applicable in IHC-P, IF, FC

Monoclonal CD6 Antibody - With BSA and Azide, Clone: SPM547
AMM00455G	Leading Biology	0.05mg	EUR 396
Description: A Monoclonal antibody against Human CD6 - With BSA and Azide. The antibodies are raised in Mouse and are from clone SPM547. This antibody is applicable in IHC-P, IF, FC

Polyclonal IDH1 (Isocitrate Dehydrogenase) Antibody - With BSA and Azide
APR07378G	Leading Biology	0.05mg	EUR 396
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human IDH1 (Isocitrate Dehydrogenase) - With BSA and Azide. This antibody is tested and proven to work in the following applications:

Monoclonal CD28 Antibody - With BSA and Azide, Clone: CB28
APR14440G	Leading Biology	0.05mg	EUR 396
Description: A Monoclonal antibody against Human CD28 - With BSA and Azide. The antibodies are raised in Mouse and are from clone CB28. This antibody is applicable in IHC, IF, FC, IP, E

Monoclonal CD6 Antibody - With BSA and Azide, Clone: 3F7B5
APR14460G	Leading Biology	0.05mg	EUR 396
Description: A Monoclonal antibody against Human CD6 - With BSA and Azide. The antibodies are raised in Mouse and are from clone 3F7B5. This antibody is applicable in IHC

Monoclonal Moesin Antibody - With BSA and Azide, Clone: SPM562
APR14681G	Leading Biology	0.05mg	EUR 396
Description: A Monoclonal antibody against Human Moesin - With BSA and Azide. The antibodies are raised in Mouse and are from clone SPM562. This antibody is applicable in IHC-P, IF, FC

DiagPoly Carboxyl Fluorescent Polystyrene Particles, Nile Red, 0.09-0.3 µm
DNM-L071	Creative Diagnostics	2 mL	EUR 657

Normal Rabbit Serum (Blocking Solution)
AR1010	BosterBio	100mL (for 10000-15000 assays)	EUR 131

Rabbit Serum Albumin ELISA kit
E04S0014-192T	B-Gene	192 tests	EUR 1270
Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT Show more
Description: A competitive ELISA for quantitative measurement of Rabbit Serum Albumin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Serum Albumin ELISA kit
E04S0014-48	B-Gene	1 plate of 48 wells	EUR 520
Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT Show more
Description: A competitive ELISA for quantitative measurement of Rabbit Serum Albumin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Serum Albumin ELISA kit
E04S0014-96	B-Gene	1 plate of 96 wells	EUR 685
Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT Show more
Description: A competitive ELISA for quantitative measurement of Rabbit Serum Albumin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Serum amyloid ELISA kit
E04S0228-192T	B-Gene	192 tests	EUR 1270
Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT Show more
Description: A competitive ELISA for quantitative measurement of Rabbit Serum amyloid in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Serum amyloid ELISA kit
E04S0228-48	B-Gene	1 plate of 48 wells	EUR 520
Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT Show more
Description: A competitive ELISA for quantitative measurement of Rabbit Serum amyloid in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Serum amyloid ELISA kit
E04S0228-96	B-Gene	1 plate of 96 wells	EUR 685
Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT Show more
Description: A competitive ELISA for quantitative measurement of Rabbit Serum amyloid in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Serum Iron ELISA kit
E04S0273-192T	B-Gene	192 tests	EUR 1270
Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT Show more
Description: A competitive ELISA for quantitative measurement of Rabbit Serum Iron in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Serum Iron ELISA kit
E04S0273-48	B-Gene	1 plate of 48 wells	EUR 520
Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT Show more
Description: A competitive ELISA for quantitative measurement of Rabbit Serum Iron in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Serum Iron ELISA kit
E04S0273-96	B-Gene	1 plate of 96 wells	EUR 685
Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT Show more
Description: A competitive ELISA for quantitative measurement of Rabbit Serum Iron in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Serum Albumin Polyclonal Antibody
A57592	EpiGentek	100 µg	EUR 570.55
Description: fast delivery possible

Rabbit anti Bovine Serum antibody
40R-RB001	Fitzgerald	2 ml	EUR 241
Description: Rabbit anti Bovine Serum antibody

Pre-immune normal rabbit serum
NRS01	GroPep	200 µl	EUR 299

Rabbit serum albumin lyophilized powder
RSA62-0001	Equitech	1gm	EUR 231.4
Rabbit serum albumin lyophilized powder is indicated as RUO. Do not use on humans.

Rabbit serum albumin lyophilized powder
RSA62-0010	Equitech	10gm	EUR 839.8
Rabbit serum albumin lyophilized powder is indicated as RUO. Do not use on humans.

Rabbit serum albumin lyophilized powder
RSA62-0100	Equitech	100gm	Ask for price
Rabbit serum albumin lyophilized powder is indicated as RUO. Do not use on humans.

Rabbit serum albumin lyophilized powder
RSA62-1000	Equitech	1KG	Ask for price
Rabbit serum albumin lyophilized powder is indicated as RUO. Do not use on humans.

Limited Tryptic Digestion-Isotope Dilution Mass Spectrometry (LTD-IDMS): A Reagent-Free Analytical Assay To Quantify Hemagglutinin of A(H5N1) Vaccine Material

• Avian influenza viruses, such as A(H5N1) and A(H7N9), are primary public health concerns due to their pandemic potential. Influenza vaccines represent the most effective response to this threat especially with timely provision. The current pandemic response timelines require a substantial period for strain-specific reference antigen and sera preparation for use with single-radial immunodiffusion (SRID), the accepted vaccine potency assay.
• To address this time lag, the isotope dilution mass spectrometry (IDMS) method was developed to quantify the absolute hemagglutinin (HA, the main influenza antigen) amount in the vaccine without the need for purified, inactivated, and calibrated virus reference antigens. However, an additional challenge in determining potency is to differentiate between vaccine antigens in their most potent form from other less potent, stressed antigen forms.
• The limited trypsin digestion (LTD) method has been developed and does not require strain-specific full-length reference antigens or antibodies; instead, stressed HA is selectively degraded, leaving the more potent form to be measured. LTD, followed by precipitation and IDMS, allows for efficient differentiation between potent and significantly less potent HA for vaccine release and potency testing across the vaccine’s shelf life.
• In this study, we tested the LTD-IDMS assay on A(H5N1) vaccine material that had been stressed by low pH, heat, and multiple freeze-thaw cycles. The results showed that the LTD-IDMS method effectively quantified the potent HA in A(H5N1) vaccine material with results comparable to SRID. As such, it shows great promise to complement and potentially replace SRID in a pandemic when strain-specific reagents may not be readily available.

Translation of ceragenin affinity for bacteria to an imaging reagent for infection

Responses to bacterial infections may be manifest systemically without evidence of the location of the infection site. A rapid means of pinpointing infection sites would be useful in providing effective and possibly localized treatment. Successful means of identifying infection sites would require two components: (1) a molecule capable of recognizing bacteria and (2) a means of communicating recognition.

For the recognition element, we used a ceragenin, a small molecule with affinity for bacterial membranes that was designed as a mimic of endogenous antimicrobial peptides. For the communication element, we used ⁶⁴Cu, which is a positron emitter. By conjugating a copper chelating group to the ceragenin, the two elements were combined. Chelation of ⁶⁴Cu by the conjugate was effective and provided a stable complex that allowed in vivo imaging.

When administered to mice in a thigh infection model, the ⁶⁴Cu-labeled conjugate accumulated at the site of infection (right thigh) without accumulation at the complementary site (left thigh). This conjugate may provide a means of identifying infection sites in patients presenting general signs of infection without localized symptoms.

Mouse Serum
20-abx098261	Abbexa	EUR 1163.00 EUR 272.00	100 ml 10 ml
Shipped within 5-10 working days.

Mouse Serum
abx098261-5L	Abbexa	5 L	EUR 8833
Shipped within 5-10 working days.

Mouse Serum
abx098262-1ml	Abbexa	1 ml	EUR 1720
Shipped within 5-10 working days.

Normal Mouse Serum
88-NM35	Fitzgerald	50 ml	EUR 516
Description: Normal Mouse Serum

Normal Mouse Serum
88R-1002	Fitzgerald	5 ml	EUR 203
Description: Normal Mouse Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Mouse Serum Albumin
30R-3304	Fitzgerald	10 mg	EUR 187
Description: Purified native Mouse Serum Albumin

Mouse Complement Serum
32R-CM001	Fitzgerald	5 ml	EUR 435
Description: Frozen high activity mouse complement serum

Mouse Serum Amyloid P Component (SAP) Reference serum
SAP12-RS	Alpha Diagnostics	100 ul	EUR 324

Equine Serum
EQS001	Neuromics	500 ml	EUR 200

Cow Serum
abx098251-100ml	Abbexa	100 ml	EUR 342
Shipped within 5-10 working days.

Fish Serum
abx098257-10ml	Abbexa	10 ml	EUR 439
Shipped within 5-10 working days.

Goat Serum
abx098258-500ml	Abbexa	500 ml	EUR 370
Shipped within 5-10 working days.

Goat Serum
abx098259-500ml	Abbexa	500 ml	EUR 370
Shipped within 5-10 working days.

Horse Serum
abx098260-100ml	Abbexa	100 ml	EUR 342
Shipped within 5-10 working days.

Porcine Serum
abx098264-1L	Abbexa	1 L	EUR 592
Shipped within 5-10 working days.

Rabbit Serum
abx098265-100ml	Abbexa	100 ml	EUR 272
Shipped within 5-10 working days.

Rabbit Serum
abx098266-500ml	Abbexa	500 ml	EUR 481
Shipped within 5-10 working days.

Rat Serum
abx098267-10ml	Abbexa	10 ml	EUR 272
Shipped within 5-10 working days.

Sheep Serum
abx098268-100ml	Abbexa	100 ml	EUR 342
Shipped within 5-10 working days.

Canine Serum
88R-D005S	Fitzgerald	50 ml	EUR 154
Description: Non Sterile Canine Serum

Rabbit Serum
1267-1	Biovision		EUR 157

Chicken Serum
SER001	Neuromics	500 ml	EUR 265

Mouse Serum Albumin Protein
20-abx600021	Abbexa	EUR 926.00 EUR 314.00 EUR 3975.00 EUR 244.00	100 mg 10 mg 1 g 1 mg
Shipped within 1 month.

Mouse Serum Albumin antibody
70R-AG001	Fitzgerald	1 mg	EUR 256
Description: Affinity purified Goat polyclonal Mouse Serum Albumin antibody

Mouse Serum Albumin protein
30-1179	Fitzgerald	10 mg	EUR 236
Description: Purified native Mouse Albumin protein

Mouse Serum Albumin antibody
20R-AR005	Fitzgerald	20 mg	EUR 327
Description: Rabbit polyclonal Mouse Serum Albumin antibody

Mouse Serum albumin antibody
20R-AR041	Fitzgerald	2 ml	EUR 295
Description: Rabbit polyclonal Mouse Serum albumin antibody

Mouse Serum albumin (Alb)
1-CSB-YP001561MO	Cusabio	EUR 586.00 EUR 299.00 EUR 2172.00 EUR 900.00 EUR 1442.00 EUR 382.00	100ug 10ug 1MG 200ug 500ug 50ug
MW: 67.9 kDa Buffer composition: Tris-based buffer with 50% glycerol.
Description: Recombinant Mouse Serum albumin(Alb) expressed in Yeast

Mouse Serum albumin (Alb)
1-CSB-BP001561MO	Cusabio	EUR 840.00 EUR 332.00 EUR 2170.00 EUR 1135.00 EUR 1579.00 EUR 470.00	100ug 10ug 1MG 200ug 500ug 50ug
MW: 67.9 kDa Buffer composition: Tris-based buffer with 50% glycerol.
Description: Recombinant Mouse Serum albumin(Alb) expressed in Baculovirus

Non-sterile mouse serum
MS05-0050	Equitech	50 ml	EUR 231.4
Non-sterile mouse serum is indicated as RUO. Do not use on humans.

Non-sterile mouse serum
MS05-0100	Equitech	100 ml	EUR 257.4
Non-sterile mouse serum is indicated as RUO. Do not use on humans.

Non-sterile mouse serum
MS05-0500	Equitech	500 ml	EUR 440.7
Non-sterile mouse serum is indicated as RUO. Do not use on humans.

Exo-Flow 2.0 Basic Kit without antibody (Streptavidin beads + reagents) - for Serum or Plasma
EXOFLOW2-BASICA-SP	SBI	30 rxn	EUR 1001
Category: Exosome

Rabbit Serum (Sterile)
20-abx800002	Abbexa	EUR 1400.00 EUR 3293.00 EUR 829.00	100 ml 500 ml 50 ml
Shipped within 1-5 working days.

Rabbit Serum (Sterile)
abx800002-1L	Abbexa	1 L	EUR 5437
Shipped within 1-5 working days.

Sheep Serum (Sterile)
20-abx800019	Abbexa	EUR 592.00 EUR 759.00 EUR 425.00	100 ml 500 ml 50 ml
Shipped within 1-5 working days.

Sheep Serum (Sterile)
abx800019-1L	Abbexa	1 L	EUR 996
Shipped within 1-5 working days.

Goat Serum (Sterile)
20-abx800034	Abbexa	EUR 606.00 EUR 773.00 EUR 439.00	100 ml 500 ml 50 ml
Shipped within 1-5 working days.

Goat Serum (Sterile)
abx800034-1L	Abbexa	1 L	EUR 926
Shipped within 1-5 working days.

Horse Serum (Sterile)
20-abx800049	Abbexa	EUR 829.00 EUR 1080.00 EUR 578.00	100 ml 500 ml 50 ml
Shipped within 1-5 working days.

Horse Serum (Sterile)
abx800049-1L	Abbexa	1 L	EUR 1316
Shipped within 1-5 working days.

Donkey Serum (Sterile)
20-abx800064	Abbexa	EUR 1358.00 EUR 1803.00 EUR 746.00	100 ml 500 ml 50 ml
Shipped within 1-5 working days.

Donkey Serum (Sterile)
abx800064-1L	Abbexa	1 L	EUR 2221
Shipped within 1-5 working days.